He-Ne laser effects on blood microcirculation during wound healing: A method of in vivo study through laser Doppler flowmetry

2004 ◽  
Vol 35 (5) ◽  
pp. 363-368 ◽  
Author(s):  
Silvia Cristina Núñez ◽  
Gessé E.C. Nogueira ◽  
Martha S. Ribeiro ◽  
Aguinaldo S. Garcez ◽  
José L. Lage-Marques
Keyword(s):  
Wound Healing ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
In Vivo Study ◽  
Doppler Flowmetry ◽  
Blood Microcirculation

scholarly journals A Minimally Invasive Technique for Serial Intraosseous Perfusion Measurements in the Murine Tibia Using Laser Doppler Flowmetry

2019 ◽  
Author(s):  
Nicholas J Hanne ◽  
Elizabeth D Easter ◽  
Sandra Stangeland-Molo ◽  
Jacqueline H Cole
Keyword(s):  
Minimally Invasive ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Invasive Technique ◽  
List Type ◽  
Current Standard ◽  
Preclinical Research ◽  
Modified Technique ◽  
Doppler Flowmetry

AbstractIn biomedical and preclinical research, the current standard method for measuring blood perfusion inside murine bone, radiolabeled microspheres, is a terminal procedure that cannot be used to monitor longitudinal perfusion changes. Laser Doppler flowmetry (LDF) can quantify perfusion within the proximal tibial metaphysis of mice in vivo but requires a surgical procedure to place the measurement probe directly onto the bone surface. Sustained inflammation for over a month following this technique was previously reported, and previous studies have used LDF as an endpoint-only procedure. We developed a modified, minimally invasive LDF procedure to measure intraosseous perfusion in the murine tibia without stimulating local or systemic inflammation or inducing gait abnormalities. This modified technique can be used to measure perfusion weekly for up to at least a month. Unlike previous endpoint-only techniques, this modified LDF procedure can be performed weekly to monitor serial changes to intraosseous perfusion in the murine tibiaThe modified LDF technique utilizes a smaller, more localized incision to minimize invasiveness and speed recovery

Laser-Doppler Flowmetry in the Study of in Vivo Liver Ischemia and Reperfusion in the Rat

1994 ◽  
Vol 56 (5) ◽  
pp. 473-477 ◽  
Author(s):  
Rafael E. Chávez-Cartaya ◽  
Pablo Ramirez-Romero ◽  
Sir Roy Y. Calne ◽  
Neville V. Jamieson
Keyword(s):  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Ischemia And Reperfusion ◽  
Liver Ischemia ◽  
Doppler Flowmetry

scholarly journals About the in vivo discriminatory capacity of photoplethysmography versus laser Doppler flowmetry

2017 ◽  
Vol 14 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Clemente Rocha ◽  
Henrique Silva ◽  
Hugo Ferreira ◽  
L Monteiro Rodrigues
Keyword(s):  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
Discriminatory Capacity

scholarly journals In-Vivo Measurement of Rotator Cuff Perfusion Using Laser Doppler Flowmetry (SS-38)

2007 ◽  
Vol 23 (6) ◽  
pp. e19-e20 ◽  
Author(s):  
Kenneth J. Accousti ◽  
James Gladstone ◽  
Bradford Parsons ◽  
Raymond Klug ◽  
Evan Flatow
Keyword(s):  
Rotator Cuff ◽  
Laser Doppler Flowmetry ◽  
Laser Doppler ◽  
Doppler Flowmetry ◽  
In Vivo Measurement

Cold-induced cutaneous vasoconstriction is mediated by Rho kinase in vivo in human skin

2007 ◽  
Vol 292 (4) ◽  
pp. H1700-H1705 ◽  
Author(s):  
Caitlin S. Thompson-Torgerson ◽  
Lacy A. Holowatz ◽  
Nicholas A. Flavahan ◽  
W. Larry Kenney
Keyword(s):  
Laser Doppler Flowmetry ◽  
Combined Treatment ◽  
Rho Kinase ◽  
Laser Doppler ◽  
Whole Body ◽  
Local Skin ◽  
Doppler Flowmetry ◽  
Thermoregulatory Model ◽  
Cutaneous Vasoconstriction

Cutaneous vasoconstriction (VC) is the initial thermoregulatory response to cold exposure and can be elicited through either whole body or localized skin cooling. However, the mechanisms governing local cold-induced VC are not well understood. We tested the hypothesis that Rho kinase participates in local cold-induced cutaneous VC. In seven men and women (20–27 yr of age), up to four ventral forearm skin sites were instrumented with intradermal microdialysis fibers for localized drug delivery during cooling. Skin blood flow was monitored at each site with laser-Doppler flowmetry while local skin temperature was decreased and maintained at 24°C for 40 min. Cutaneous vascular conductance (CVC; laser-Doppler flowmetry/mean arterial pressure) was expressed as percent change from 34°C baseline. During the first 5 min of cooling, CVC decreased at control sites (lactated Ringer solution) to −45 ± 6% ( P < 0.001), increased at adrenoceptor-antagonized sites (yohimbine + propranolol) to 15 ± 14% ( P = 0.002), and remained unchanged at both Rho kinase-inhibited (fasudil) and adrenoceptor-antagonized + Rho kinase-inhibited sites (yohimbine + propranolol + fasudil) (−9 ± 1%, P = 0.4 and −6 ± 2%, P = 0.4, respectively). During the last 5 min of cooling, CVC further decreased at all sites when compared with baseline values (control, −77 ± 4%, P < 0.001; adrenoceptor antagonized, −61 ± 3%, P < 0.001; Rho kinase inhibited, −34 ± 7%, P < 0.001; and adrenoceptor antagonized + Rho kinase inhibited sites, −35 ± 3%, P < 0.001). Rho kinase-inhibited and combined treatment sites were significantly attenuated when compared with both adrenoceptor-antagonized ( P < 0.01) and control sites ( P < 0.0001). Rho kinase mediates both early- and late-phase cold-induced VC, supporting in vitro findings and providing a putative mechanism through which both adrenergic and nonadrenergic cold-induced VC occurs in an in vivo human thermoregulatory model.

scholarly journals STUDY OF HEMOMICROCIRCULATION IN UPPER- EXTREMITYSKIN IN HEALTHY MEN IN NORMAL CONDITIONS WITH ACCOUNT OF H

2020 ◽  
Vol 10 (4) ◽  
pp. 63-65
Author(s):  
Vadim Astashov ◽  
Valentin Kozlov ◽  
Victor Sidorov, ◽  
Mihail Uloga ◽  
Inna Borodina ◽  
...  
Keyword(s):  
Blood Flow ◽  
Laser Doppler Flowmetry ◽  
Peripheral Blood ◽  
Upper Extremities ◽  
Laser Doppler ◽  
Peripheral Blood Flow ◽  
Doppler Flowmetry ◽  
Healthy Men ◽  
Young Males ◽  
Blood Microcirculation

In this study we used laser doppler flowmetry to investigate the parameters of peripheral blood flow in the upper extremities in young males both right- and left-handers. Based on the data obtained we found that in right-handers (dextrals) active mechanisms of regulation of blood microcirculation prevail on the leading hand, In left-handers (sinestrals) active and passive mechanisms of its regulation are involved in the regulation of blood flow on the leading hand (left) and on the opposite (right). However, the contribution of active mechanisms is lower than that of right-handers.

Sign in / Sign up

Export Citation Format

Share Document