scholarly journals A sensitive fluorescent assay for measuring carbon‐phosphorus lyase activity in aquatic systems

2021 ◽  
Author(s):  
Benjamin N. Granzow ◽  
Oscar A. Sosa ◽  
Margherita Gonnelli ◽  
Chiara Santinelli ◽  
David M. Karl ◽  
...  
Keyword(s):  
Aquatic Systems ◽  
Fluorescent Assay ◽  
Lyase Activity ◽  
Carbon Phosphorus Lyase

Effect of short-term light- and UV-stress on DMSP, DMS, and DMSP lyase activity in Emiliania huxleyi

2015 ◽  
Vol 74 (2) ◽  
pp. 173-185 ◽  
Author(s):  
LJ Darroch ◽  
M Lavoie ◽  
M Levasseur ◽  
I Laurion ◽  
WG Sunda ◽  
...  
Keyword(s):  
Emiliania Huxleyi ◽  
Short Term ◽  
Lyase Activity ◽  
Uv Stress

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

Online Measurement of Glucose Consumption from HepG2 Cells Using an Integrated Bioreactor and Enzymatic Assay

2018 ◽  
Author(s):  
Anna Adams ◽  
Radha Krishna Murthy Bulusu ◽  
Nikita Mukhitov ◽  
Jose Mendoza-Cortes ◽  
Michael Roper
Keyword(s):  
Real Time ◽  
Glucose Concentration ◽  
Hepg2 Cells ◽  
Glucose Consumption ◽  
Microfluidic System ◽  
Structure And Function ◽  
Online Measurement ◽  
Fluorescent Assay ◽  
And Function ◽  
Integrated Bioreactor

In this work, we developed a microfluidic bioreactor for optimizing growth and maintaining structure and function of HepG2, and when desired, the device could be removed and the extracellular output from the bioreactor combined with enzymatic glucose reagents into a droplet-based microfluidic system. The intensity of the resulting fluorescent assay product in the droplets was measured, and was directly correlated to glucose concentration, allowing the effect of insulin on glucose consumption in the HepG2 cells to be observed and quantified online and in near real-time.

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