Development of a Novel High‐Performance Thin Layer Chromatography–Based Method for the Simultaneous Quantification of Clinically Relevant Lipids from Cells and Tissue Extracts

Lipids ◽  
2020 ◽  
Vol 55 (4) ◽  
pp. 403-412
Author(s):  
Michelle Pinault ◽  
Cyrille Guimaraes ◽  
Céline Ben Hassen ◽  
Jorge L. Gutierrez‐Pajares ◽  
Stéphan Chevalier ◽  
...  
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Simultaneous Quantification ◽  
Tissue Extracts ◽  
Layer Chromatography

scholarly journals Simultaneous Determination of Bergenin and Gallic Acid in Bergenia ligulata Wall by High-Performance Thin-Layer Chromatography

2000 ◽  
Vol 83 (6) ◽  
pp. 1480-1483 ◽  
Author(s):  
Sateesh K Chauhan ◽  
Brijpal Singh ◽  
Sudhakar Agrawal
Keyword(s):  
Thin Layer ◽  
Gallic Acid ◽  
Thin Layer Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Solvent System ◽  
Simultaneous Quantification ◽  
Acetic Acid Water ◽  
Layer Chromatography

Abstract A simple and reproducible high-performance thin-layer chromatographic method was developed for the simultaneous determination of bergenin and gallic acid in Bergenia ligulata. Water and methanol were used as the extracting solvents. The concentrations of bergenin and gallic acid in both of these solvents were found to be almost the same. The method involves separation of the components by thin-layer chromatography on a precoated Silica Gel 60 F254 plate with a solvent system of ethyl acetate–formic acid–acetic acid–water (100 + 11 + 11 + 27). The sensitivity of the method for bergenin was 0.30 μg, whereas for gallic acid it was 0.25 μg. The proposed method is precise and sensitive and can be used for the detection, monitoring, and simultaneous quantification of bergenin and gallic acid in B. ligulata.

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