Humanized Anti‐DNABII Fab Fragments Plus Ofloxacin Eradicated Biofilms in Experimental Otitis Media

2021 ◽  
Author(s):  
Laura A. Novotny ◽  
Tendy Chiang ◽  
Steven D. Goodman ◽  
Charles A. Elmaraghy ◽  
Lauren O. Bakaletz
Keyword(s):  
Otitis Media ◽  
Fab Fragments

Large-cluster and combined fluorescent and gold immunoprobes

1996 ◽  
Vol 54 ◽  
pp. 892-893
Author(s):  
Richard D. Powell ◽  
James F. Hainfeld ◽  
Carol M. R. Halsey ◽  
David L. Spector ◽  
Shelley Kaurin ◽  
...  
Keyword(s):  
Metal Cluster ◽  
Sulfonyl Chloride ◽  
Gel Filtration ◽  
Cross Linking ◽  
Site Specific ◽  
Fab Fragments ◽  
Cluster Label ◽  
Covalently Linked ◽  
Texas Red ◽  
Fold Excess

Two new types of covalently linked, site-specific immunoprobes have been prepared using metal cluster labels, and used to stain components of cells. Combined fluorescein and 1.4 nm “Nanogold” labels were prepared by using the fluorescein-conjugated tris (aryl) phosphine ligand and the amino-substituted ligand in the synthesis of the Nanogold cluster. This cluster label was activated by reaction with a 60-fold excess of (sulfo-Succinimidyl-4-N-maleiniido-cyclohexane-l-carboxylate (sulfo-SMCC) at pH 7.5, separated from excess cross-linking reagent by gel filtration, and mixed in ten-fold excess with Goat Fab’ fragments against mouse IgG (obtained by reduction of F(ab’)2 fragments with 50 mM mercaptoethylamine hydrochloride). Labeled Fab’ fragments were isolated by gel filtration HPLC (Superose-12, Pharmacia). A combined Nanogold and Texas Red label was also prepared, using a Nanogold cluster derivatized with both and its protected analog: the cluster was reacted with an eight-fold excess of Texas Red sulfonyl chloride at pH 9.0, separated from excess Texas Red by gel filtration, then deprotected with HC1 in methanol to yield the amino-substituted label.

Myringotomy in acute otitis media. A controlled study

JAMA ◽  
1966 ◽  
Vol 197 (11) ◽  
pp. 849-853 ◽  
Author(s):  
O. F. Roddey
Keyword(s):  
Otitis Media ◽  
Acute Otitis Media ◽  
Controlled Study

The Concept of Pronicity in Otitis Media

1991 ◽  
Vol 24 (4) ◽  
pp. 787-794 ◽  
Author(s):  
Karin Prellner ◽  
Olof Kalm ◽  
Göran Harsten
Keyword(s):  
Otitis Media

The Concept of Silent Otitis Media

1991 ◽  
Vol 24 (4) ◽  
pp. 763-774 ◽  
Author(s):  
Michael M. Paparella ◽  
Barry P. Kimberley ◽  
Michael Alieva
Keyword(s):  
Otitis Media

Screening von Kindern in den ersten vier Lebensjahren zwecks frühzeitiger Behandlung einer Otitis media mit Erguss

Praxis ◽  
2003 ◽  
Vol 92 (45) ◽  
pp. 1909-1909
Author(s):  
Butler ◽  
van der Linden ◽  
MacMillan ◽  
van der Wouden
Keyword(s):  
Otitis Media

Topische Analgesie zur Behandlung der akuten Otitis media

Praxis ◽  
2007 ◽  
Vol 96 (50) ◽  
pp. 2013-2013
Author(s):  
Foxlee ◽  
Johansson ◽  
Wejfalk ◽  
Dawkins ◽  
Dooley ◽  
...  
Keyword(s):  
Otitis Media
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