In vivo vocal fold cover layer replacement

2014 ◽  
Vol 125 (2) ◽  
pp. 406-411 ◽  
Author(s):  
Jennifer Long ◽  
Jonathan Salinas ◽  
Sassan Rafizadeh ◽  
Georg Luegmair ◽  
Zhaoyan Zhang ◽  
...  
Keyword(s):  
Vocal Fold ◽  
Cover Layer ◽  
Fold Cover

Vocal Fold Cover Layer with a Tissue-Engineered Structure Containing Epithelium and Fibroblast of Oral Mucosa

2013 ◽  
Vol 149 (2_suppl) ◽  
pp. P214-P214
Author(s):  
Shunichi Chitose ◽  
Mioko Fukahori ◽  
Shintaro Sueyoshi ◽  
Takashi Kurita ◽  
Kiminori Sato ◽  
...  
Keyword(s):  
Oral Mucosa ◽  
Vocal Fold ◽  
Cover Layer ◽  
Fold Cover

scholarly journals Unraveling the molecular pathobiology of vocal fold systemic dehydration using an in vivo rabbit model

PLoS ONE ◽  
2020 ◽  
Vol 15 (7) ◽  
pp. e0236348
Author(s):  
Naila Cannes do Nascimento ◽  
Andrea P. dos Santos ◽  
M. Preeti Sivasankar ◽  
Abigail Cox
Keyword(s):  
Vocal Fold ◽  
Rabbit Model

scholarly journals Concurrent YAP/TAZ and SMAD signaling mediate vocal fold fibrosis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ryosuke Nakamura ◽  
Nao Hiwatashi ◽  
Renjie Bing ◽  
Carina P. Doyle ◽  
Ryan C. Branski
Keyword(s):  
Vocal Fold ◽  
Vocal Folds ◽  
Nuclear Accumulation ◽  
Smad Pathway ◽  
Surgical Interventions ◽  
Smad Signaling ◽  
Iatrogenic Damage ◽  
Tgf Β1

AbstractVocal fold (VF) fibrosis is a major cause of intractable voice-related disability and reduced quality of life. Excision of fibrotic regions is suboptimal and associated with scar recurrence and/or further iatrogenic damage. Non-surgical interventions are limited, putatively related to limited insight regarding biochemical events underlying fibrosis, and downstream, the lack of therapeutic targets. YAP/TAZ integrates diverse cell signaling events and interacts with signaling pathways related to fibrosis, including the TGF-β/SMAD pathway. We investigated the expression of YAP/TAZ following vocal fold injury in vivo as well as the effects of TGF-β1 on YAP/TAZ activity in human vocal fold fibroblasts, fibroblast-myofibroblast transition, and TGF-β/SMAD signaling. Iatrogenic injury increased nuclear localization of YAP and TAZ in fibrotic rat vocal folds. In vitro, TGF-β1 activated YAP and TAZ in human VF fibroblasts, and inhibition of YAP/TAZ reversed TGF-β1-stimulated fibroplastic gene upregulation. Additionally, TGF-β1 induced localization of YAP and TAZ in close proximity to SMAD2/3, and nuclear accumulation of SMAD2/3 was inhibited by a YAP/TAZ inhibitor. Collectively, YAP and TAZ were synergistically activated with the TGF-β/SMAD pathway, and likely essential for the fibroplastic phenotypic shift in VF fibroblasts. Based on these data, YAP/TAZ may evolve as an attractive therapeutic target for VF fibrosis.

scholarly journals Quantitative Evaluation of the In Vivo Vocal Fold Medial Surface Shape

2017 ◽  
Vol 31 (4) ◽  
pp. 513.e15-513.e23 ◽  
Author(s):  
Andrew M. Vahabzadeh-Hagh ◽  
Zhaoyan Zhang ◽  
Dinesh K. Chhetri
Keyword(s):  
Quantitative Evaluation ◽  
Vocal Fold ◽  
Surface Shape ◽  
Medial Surface

scholarly journals In vivo investigation of acidified pepsin exposure to porcine vocal fold epithelia

2015 ◽  
Vol 126 (1) ◽  
pp. E12-E17 ◽  
Author(s):  
Abigail Durkes ◽  
M. Preeti Sivasankar
Keyword(s):  
Vocal Fold

Developmental derivation of vocal fold mucosa from human induced pluripotent stem cells

2019 ◽  
Author(s):  
Vlasta Lungova ◽  
Susan Thibeault
Keyword(s):  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Vocal Fold ◽  
Fluorescent Protein ◽  
Three Dimensional ◽  
Stratified Squamous Epithelium ◽  
Three Dimensional Models ◽  
Induced Pluripotent ◽  
Green Fluorescent

Abstract Development of treatments for vocal dysphonia has been inhibited by lack of human vocal fold (VF) mucosa models because of difficulty in procuring VF epithelial cells, epithelial cells’ limited proliferative capacity and absence of cell lines. We report development of engineered VF mucosae from hiPSC, transfected via TALEN constructs for green fluorescent protein, that mimic development of VF epithelial cells in utero. Modulation of FGF signaling achieves stratified squamous epithelium from definitive and anterior foregut derived cultures. Robust culturing of these cells on collagen-fibroblast constructs produces three-dimensional models comparable to in vivo VF mucosa.

scholarly journals Toward Development of a Vocal Fold Contact Pressure Probe: Sensor Characterization and Validation Using Synthetic Vocal Fold Models

2019 ◽  
Vol 9 (15) ◽  
pp. 3002 ◽  
Author(s):  
Mohsen Motie-Shirazi ◽  
Matías Zañartu ◽  
Sean D. Peterson ◽  
Daryush D. Mehta ◽  
James B. Kobler ◽  
...  
Keyword(s):  
Contact Pressure ◽  
Vocal Fold ◽  
Ground Truth ◽  
Pressure Probe ◽  
Primary Role ◽  
Novel Approach ◽  
Sensor Position ◽  
Probe Sensor ◽  
Contact Pressures

Excessive vocal fold collision pressures during phonation are considered to play a primary role in the formation of benign vocal fold lesions, such as nodules. The ability to accurately and reliably acquire intraglottal pressure has the potential to provide unique insights into the pathophysiology of phonotrauma. Difficulties arise, however, in directly measuring vocal fold contact pressures due to physical intrusion from the sensor that may disrupt the contact mechanics, as well as difficulty in determining probe/sensor position relative to the contact location. These issues are quantified and addressed through the implementation of a novel approach for identifying the timing and location of vocal fold contact, and measuring intraglottal and vocal fold contact pressures via a pressure probe embedded in the wall of a hemi-laryngeal flow facility. The accuracy and sensitivity of the pressure measurements are validated against ground truth values. Application to in vivo approaches are assessed by acquiring intraglottal and VF contact pressures using a synthetic, self-oscillating vocal fold model in a hemi-laryngeal configuration, where the sensitivity of the measured intraglottal and vocal fold contact pressure relative to the sensor position is explored.

scholarly journals In vivo 3D human vocal fold imaging with polarization sensitive optical coherence tomography and a MEMS scanning catheter

2010 ◽  
Vol 18 (14) ◽  
pp. 14644 ◽  
Author(s):  
Ki Hean Kim ◽  
James A. Burns ◽  
Jonathan J. Bernstein ◽  
Gopi N. Maguluri ◽  
B. Hyle Park ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Vocal Fold ◽  
Optical Coherence
Sign in / Sign up

Export Citation Format

Share Document