Immunohistochemical expression patterns of the HER4 receptors in normal mucosa and in laryngeal squamous cell carcinomas: Antioncogenic significance of the HER4 protein in laryngeal squamous cell carcinoma

2012 ◽  
Vol 122 (8) ◽  
pp. 1724-1733 ◽  
Author(s):  
Francesco Bussu ◽  
Franco O. Ranelletti ◽  
Marco Gessi ◽  
Cristina Graziani ◽  
Paola Lanza ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Expression Patterns ◽  
Normal Mucosa ◽  
Squamous Cell Carcinomas ◽  
Immunohistochemical Expression ◽  
Her4 Protein

AQP1, AQP5, Bcl-2 and p16 in pharyngeal squamous cell carcinoma

2015 ◽  
Vol 129 (6) ◽  
pp. 580-586 ◽  
Author(s):  
G F Lehnerdt ◽  
H S Bachmann ◽  
M Adamzik ◽  
A Panic ◽  
E Köksal ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Patterns ◽  
Clinicopathological Characteristics ◽  
Squamous Cell Carcinomas ◽  
Clinicopathological Parameters ◽  
Immunohistochemical Expression ◽  
Poor Clinical Outcome ◽  
Personalised Treatment

AbstractObjective:This study aimed to link expression patterns of AQP1, AQP5, Bcl-2 and p16 to clinicopathological characteristics of oro-hypopharyngeal squamous cell carcinomas.Methods:Immunohistochemical expression of AQP1, AQP5, Bcl-2 and p16 was investigated in 107 consecutive oro-hypopharyngeal squamous cell carcinoma cases. Molecular interrelationship and correlations with clinicopathological parameters and survival were computed.Results:AQP1 was expressed exclusively by a subgroup of basaloid-like squamous cell carcinomas. AQP5 was detected in 25.2 per cent of the samples, showing significant association with the absence of p16 and Bcl-2 (p = 0.018; p = 0.010). In multivariate analysis, overexpression of p16 was significantly correlated with favourable overall survival (p = 0.014).Conclusion:AQP5 defined a subset of patients with Bcl-2-negative and p16-negative tumours with a poor clinical outcome. AQP1 was found to be a marker of a subgroup of aggressive basaloid-like squamous cell carcinomas. These findings suggest that AQP1 and AQP5 are interesting candidates for further studies on risk group classification and personalised treatment of oro-hypopharyngeal squamous cell carcinomas.

scholarly journals Immunohistochemical Expression of Cathepsin D in Primary and Recurrent Squamous Cell Carcinoma

2017 ◽  
Vol 18 (9) ◽  
pp. 795-801 ◽  
Author(s):  
Krishnanand P Satelur ◽  
GS Kumar
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Cathepsin D ◽  
Statistical Significance ◽  
Squamous Cell Carcinomas ◽  
P Value ◽  
Immunohistochemical Expression ◽  
Recurrent Squamous Cell Carcinoma ◽  
Limited Usefulness

ABSTRACT Aim The aim of this study is to analyze and compare the immunohistochemical expression of cathepsin B in primary oral squamous cell carcinoma (OSCC) and recurrent OSCC. Materials and methods A total of 50 cases were studied immunohistochemically for rabbit polyclonal antihuman cathepsin D expression. A total of 10 cases of breast carcinoma were taken as positive controls. Immunohistochemical staining was performed using labeled streptavidin–biotin technique. Results All the 45 cases of OSCC, both primary and recurrent cases included, showed varying grades of cathepsin D immunoreactivity. Statistical significance at 5% level was observed in cathepsin D expression between the different grades of well, moderate, and poorly differentiated primary squamous cell carcinomas. In the comparison of cathepsin D staining intensity among primary squamous cell carcinomas with and without recurrence, a statistical significance between the groups was observed when the p-value was at 10%, but the same comparison was not significant when the p-value was at 5%. Conclusion Cathepsin D expression in primary squamous cell carcinomas with recurrences was very variable as compared with primary squamous cell carcinomas without recurrences. Comparison of cathepsin D expression in primary with their recurrent counterparts showed mostly similar intensity of expression in recurrent carcinomas, thus suggesting its limited usefulness in predicting recurrence. Clinical significance Although cathepsin D might have shown limited usefulness in predicting cancer recurrence, it, however, is a proven valuable tool to detect the aggressiveness of other tumors, and if corroborated with a larger sample may hold the key to early, more effective, and more specific treatment modalities for cases of oral cancer also. How to cite this article Satelur KP, Kumar GS. Immunohistochemical Expression of Cathepsin D in Primary and Recurrent Squamous Cell Carcinoma. J Contemp Dent Pract 2017;18(9): 795-801.

scholarly journals The expression and prognostic value of stem cell markers Bmi-1, HESC5:3, and HES77 in human papillomavirus–positive and –negative oropharyngeal squamous cell carcinoma

Tumor Biology ◽  
2019 ◽  
Vol 41 (3) ◽  
pp. 101042831984047 ◽  
Author(s):  
Hesham Mohamed ◽  
Jaana Hagström ◽  
Lauri Jouhi ◽  
Timo Atula ◽  
Alhadi Almangush ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Stem Cell ◽  
Human Papillomavirus ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Expression Patterns ◽  
Squamous Cell Carcinomas ◽  
Oropharyngeal Squamous Cell Carcinoma ◽  
Stem Cell Markers ◽  
Cell Markers

Human papillomavirus is detected in over 50% of oropharyngeal squamous cell carcinomas. Human papillomavirus–positive oropharyngeal squamous cell carcinomas differ from human papillomavirus–negative tumors, and both expression patterns are classified as distinct entities. The Bmi-1 oncogene is a well-known member of the mammalian polycomb-group family. HESC5:3 and HES77 are newly developed monoclonal antibodies produced against undifferentiated embryonic stem cells. Our aim was to explore their roles in both human papillomavirus–positive and –negative oropharyngeal squamous cell carcinomas. Our cohort comprised 202 consecutive oropharyngeal squamous cell carcinoma patients diagnosed and treated with curative intent. We used tissue microarray tumor blocks to study the immunohistochemical expression of Bmi-1, HESC5:3, and HES77. We compared the expressions of these stem cell markers with p16 immunoexpression and human papillomavirus status, as well as with other characteristics of the tumor, and with patients’ clinical data and follow-up data. Human papillomavirus– and p16-positive tumors expressed less Bmi-1 and more HESC5:3 than the negative tumors. HES77 expression was high in human papillomavirus–positive oropharyngeal squamous cell carcinoma, but it did not correlate with p16 positivity. In our multivariable model, Bmi-1 and HESC5:3 were still associated with human papillomavirus, but the association between human papillomavirus and HES77 remained absent. In conclusion, Bmi-1, HESC5:3, and HES77 may have a different role in human papillomavirus–positive and human papillomavirus–negative tumors. There was no correlation between Bmi-1, HESC5:3, and HES77 expression and survival.

Immunohistochemical expression of p27kip1 in metastatic laryngeal squamous cell carcinoma

2014 ◽  
Vol 59 (2) ◽  
pp. 206-212 ◽  
Author(s):  
Magdalena Bodnar ◽  
Łukasz Szylberg ◽  
Wojciech Kaźmierczak ◽  
Andrzej Marszałek
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Immunohistochemical Expression

Topoisomerase IIa Protein Expression Patterns in Laryngeal Squamous Cell Carcinoma

2020 ◽  
Vol 40 (2) ◽  
pp. 807-811
Author(s):  
VASILEIOS PAPANIKOLAOU ◽  
ARISTEIDIS CHRYSOVERGIS ◽  
NICHOLAS MASTRONIKOLIS ◽  
EVANGELOS TSIAMBAS ◽  
VASILEIOS RAGOS ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Protein Expression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Expression Patterns ◽  
Topoisomerase Iia

scholarly journals Nuclear HER3 expression improves the prognostic stratification of patients with HER1 positive advanced laryngeal squamous cell carcinoma

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Giovanni Almadori ◽  
Antonella Coli ◽  
Eugenio De Corso ◽  
Dario Antonio Mele ◽  
Stefano Settimi ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Laryngeal Squamous Cell Carcinoma ◽  
Expression Patterns ◽  
Cox Proportional Hazards ◽  
Age At Diagnosis ◽  
Her3 Expression ◽  
Prognostic Stratification

Abstract Background Compared to the other members of human epidermal growth factor family receptors (HER), the role of HER3 has not been well defined in laryngeal cancer. The predictive and prognostic role of HER3 has been the focus of clinical attention but the research findings are contradictory, especially in laryngeal squamous cell carcinoma (LSCC). The variable localization of HER3 within cancer cells and the role of HER3 in primary and acquired resistance to HER1-targeted therapies remain unclear. Methods We performed a retrospective analysis of two cohorts of 66 homogeneous consecutive untreated primary advanced LSCC patients, in which co-expression of HER1, HER2 and HER3 receptors was investigated by semi-quantitative immunohistochemistry. The association of their pattern of expression with survival was evaluated by Kaplan–Meier and Cox’s proportional hazard analyses. Multivariable Cox proportional hazards models were developed to predict median 2- and 3-year RFS and 2.5- and 5-year OS. The Akaike information criterion technique and backwards stepwise procedure were used for model selections. The performance of the final Cox models was assessed with respect to calibration and discrimination. Results Immunohistochemical labeling for HER1 and HER2 was localized both in the cell membrane and in the cytoplasm, while HER3 labeling was observed both in the cell cytoplasm and in the nucleus. HER3 expression was inversely correlated with HER1 positivity. The expression patterns of HERs were associated with tumor differentiation. In both cohorts of patients, HER1 expression was associated with reduced relapse-free (RFS) and overall survival (OS). In HER1 positive tumors, the co-expression with nuclear HER3 was associated with better RFS and OS, compared with HER3 negative tumors or tumors expressing HER3 at cytoplasmic level. HER3 expressing tumors had a higher Geminin/MCM7 ratio than HER3 negative ones, regardless of HER1 co-expression. Multivariable analyses identified age at diagnosis, tumor site, HER1, HER3 and age at diagnosis, tumor stage, HER1, HER3, as covariates significantly associated with RFS and OS, respectively. Bootstrapping verified the good fitness of these models for predicting survivals and the optimism-corrected C-indices were 0.76 and 0.77 for RFS and OS, respectively. Conclusions Nuclear HER3 expression was strongly associated with favourable prognosis and allows to improve the prognostic stratification of patients with HER1 positive advanced LSCC carcinoma.

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