Multiphoton microscopy of unstained bladder mucosa based on two-photon excited autofluorescence and second harmonic generation

2009 ◽  
Vol 6 (1) ◽  
pp. 80-83 ◽  
Author(s):  
S.M. Zhuo ◽  
J.X. Chen ◽  
T. Luo ◽  
X.S. Jiang ◽  
S.S. Xie
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Bladder Mucosa ◽  
Two Photon

scholarly journals MULTIPHOTON MICROSCOPIC IMAGING OF MOUSE INTESTINAL MUCOSA BASED ON TWO-PHOTON EXCITED FLUORESCENCE AND SECOND HARMONIC GENERATION

2013 ◽  
Vol 06 (01) ◽  
pp. 1350004 ◽  
Author(s):  
REN'AN XU ◽  
XIAOQIN ZHU ◽  
NING HE ◽  
SHUANGMU ZHUO ◽  
JIAN XU ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Tissue Architecture ◽  
Intestinal Tissue ◽  
Two Photon ◽  
Main Components ◽  
Columnar Cells

Multiphoton microscopy (MPM), based on two-photon excited fluorescence and second harmonic generation, enables direct noninvasive visualization of tissue architecture and cell morphology in live tissues without the administration of exogenous contrast agents. In this paper, we used MPM to image the microstructures of the mucosa in fresh, unfixed, and unstained intestinal tissue of mouse. The morphology and distribution of the main components in mucosa layer such as columnar cells, goblet cells, intestinal glands, and a little collagen fibers were clearly observed in MPM images, and then compared with standard H&E images from paired specimens. Our results indicate that MPM combined with endoscopy and miniaturization probes has the potential application in the clinical diagnosis and in vivo monitoring of early intestinal cancer.

Jadassohn-Pellizzari anetoderma: Study of multiphoton microscopy based on two-photon excited fluorescence and second harmonic generation

2009 ◽  
Vol 19 (6) ◽  
pp. 570-575 ◽  
Author(s):  
Jingjun Zhao ◽  
Jianxin Chen ◽  
Yinghong Yang ◽  
Shuangmu Zhuo ◽  
Xingshan Jiang ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Multiphoton Microscopy ◽  
Two Photon

Extracting diagnostic stromal organization features based on intrinsic two-photon excited fluorescence and second-harmonic generation signals

2009 ◽  
Vol 14 (2) ◽  
pp. 020503 ◽  
Author(s):  
Shuangmu Zhuo ◽  
Jianxin Chen ◽  
Shusen Xie ◽  
Zhibin Hong ◽  
Xingshan Jiang
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon

scholarly journals Multiphoton Microscopy for Ophthalmic Imaging

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Emily A. Gibson ◽  
Omid Masihzadeh ◽  
Tim C. Lei ◽  
David A. Ammar ◽  
Malik Y. Kahook
Keyword(s):  
Harmonic Generation ◽  
Second Harmonic ◽  
Imaging Techniques ◽  
Multiphoton Microscopy ◽  
Tissue Imaging ◽  
Third Harmonic ◽  
Two Photon ◽  
Disease Mechanisms ◽  
Ophthalmic Imaging ◽  
Anti Stokes

We review multiphoton microscopy (MPM) including two-photon autofluorescence (2PAF), second harmonic generation (SHG), third harmonic generation (THG), fluorescence lifetime (FLIM), and coherent anti-Stokes Raman Scattering (CARS) with relevance to clinical applications in ophthalmology. The different imaging modalities are discussed highlighting the particular strength that each has for functional tissue imaging. MPM is compared with current clinical ophthalmological imaging techniques such as reflectance confocal microscopy, optical coherence tomography, and fluorescence imaging. In addition, we discuss the future prospects for MPM in disease detection and clinical monitoring of disease progression, understanding fundamental disease mechanisms, and real-time monitoring of drug delivery.

scholarly journals Multimodal two-photon imaging using a second harmonic generation-specific dye

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Mutsuo Nuriya ◽  
Shun Fukushima ◽  
Atsuya Momotake ◽  
Takanori Shinotsuka ◽  
Masato Yasui ◽  
...  
Keyword(s):  
Second Harmonic Generation ◽  
Harmonic Generation ◽  
Second Harmonic ◽  
Two Photon ◽  
Photon Imaging ◽  
Two Photon Imaging
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