scholarly journals Klotho/FGF23 axis mediates high phosphate‐induced vascular calcification in vascular smooth muscle cells via Wnt7b/β‐catenin pathway

2019 ◽  
Author(s):  
Yan‐Xia Chen ◽  
Chong Huang ◽  
Zhi‐Bing Duan ◽  
Cheng‐Yun Xu ◽  
Yan Chen
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
High Phosphate

Fetuin-A decrease induced by a low-protein diet enhances vascular calcification in uremic rats with hyperphosphatemia

2015 ◽  
Vol 309 (8) ◽  
pp. F744-F754 ◽  
Author(s):  
Shunsuke Yamada ◽  
Masanori Tokumoto ◽  
Kazuhiko Tsuruya ◽  
Narihito Tatsumoto ◽  
Hideko Noguchi ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Calcium Phosphate ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Fetuin A ◽  
Low Protein ◽  
High Phosphate

Although dietary phosphate restriction is important for treating hyperphosphatemia in patients with chronic kidney disease, it remains unclear whether a low-protein diet (LPD), which contains low phosphate, has beneficial effects on malnutrition, inflammation, and vascular calcification. The effects of LPD on inflammation, malnutrition, and vascular calcification were therefore assessed in rats. Rats were fed a normal diet or diets containing 0.3% adenine and low/normal protein and low/high phosphate. After 6 wk, serum and urinary biochemical parameters, systemic inflammation, and vascular calcification were examined. The protective effect of fetuin-A and albumin were assessed in cultured vascular smooth muscle cells. Rats fed the diet containing 0.3% adenine developed severe azotemia. LPD in rats fed high phosphate induced malnutrition (decreases in body weight, food intake, serum albumin and fetuin-A levels, and urinary creatinine excretion) and systemic inflammation (increases in serum tumor necrosis factor-α and urinary oxidative stress marker). LPD decreased the serum fetuin-A level and fetuin-A synthesis in the liver and increased serum calcium-phosphate precipitates. A high-phosphate diet increased aortic calcium content, which was enhanced by LPD. Reduced fetal calf serum in the medium of cultured vascular smooth muscle cells enhanced phosphate-induced formation of calcium-phosphate precipitates in the media and calcification of vascular smooth muscle cells, both of which were prevented by fetuin-A administration. Our results suggest that phosphate restriction by restricting dietary protein promotes vascular calcification by lowering the systemic fetuin-A level and increasing serum calcium-phosphate precipitates and induces inflammation and malnutrition in uremic rats fed a high-phosphate diet.

scholarly journals Vascular calcification is coupled with phenotypic conversion of vascular smooth muscle cells through Klf5-mediated transactivation of the Runx2 promoter

2014 ◽  
Vol 34 (6) ◽  
Author(s):  
Jing Zhang ◽  
Bin Zheng ◽  
Pei-pei Zhou ◽  
Ruo-Nan Zhang ◽  
Ming He ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
High Phosphate

High phosphate induces the expression of Klf5 and VSMC calcification. Klf5 binds directly to the Runx2 promoter and activates its transcription. Vascular calcification is coupled with phenotype conversion of VSMCs through Klf5-mediated transactivation of Runx2 promoter.

scholarly journals MicroRNA-223-3p inhibits vascular calcification and the osteogenic switch of vascular smooth muscle cells

2021 ◽  
Vol 296 ◽  
pp. 100483
Author(s):  
Yingchun Han ◽  
Jichao Zhang ◽  
Shan Huang ◽  
Naixuan Cheng ◽  
Congcong Zhang ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Calcification ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells

scholarly journals SP362EFFECT OF IRON CITRATE ON MATRIX AND SIMIL-OSTEOBLASTIC DIFFERENTIATION IN HIGH PHOSPHATE TREATED VASCULAR SMOOTH MUSCLE CELLS

2017 ◽  
Vol 32 (suppl_3) ◽  
pp. iii233-iii233
Author(s):  
Paola Ciceri ◽  
Francesca Elli ◽  
Monica Falleni ◽  
Delfina Tosi ◽  
Gaetano Bulfamante ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Osteoblastic Differentiation ◽  
Iron Citrate ◽  
High Phosphate
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