Ultrasound Biomicroscopy and High‐Frequency Ultrasound for Evaluating Extramammary Paget Disease With Pathologic Correlation

2019 ◽  
Vol 38 (12) ◽  
pp. 3229-3237 ◽  
Author(s):  
Si‐Tong Chen ◽  
Le‐Hang Guo ◽  
Jian‐Na Yan ◽  
Qiao Wang ◽  
Xiao‐Long Li ◽  
...  
2017 ◽  
Vol 37 (suppl_1) ◽  
Author(s):  
Samuel Röhl ◽  
Linnea Eriksson ◽  
Robert Saxelin ◽  
Mariette Lengquist ◽  
Kenneth Caidahl ◽  
...  

Objective: Ultrasound BioMicroscopy (UBM), or high-frequency ultrasound, is a novel technique used for assessment of anatomy and physiology small research animals. In this study, we evaluate the UBM assessment of the re-endothelialization process following denudation of the carotid artery in rats. Methods: Ultrasound BioMicroscopy data from three different experiments were analyzed. A total of 66 rats of three different strains (Sprague-Dawley, Wistar and Goto-Kakizaki) were included in this study. All animals were subjected to common carotid artery balloon injury and examined with UBM 2 and 4 weeks after injury. Re-endothelialization in UBM was measured as the length from the carotid bifurcation to the distal edge of the intimal hyperplasia. En face staining with Evans-blue dye was performed upon euthanization at 4 weeks after injury followed by tissue harvest for morphological and immunohistochemical evaluation. Results: A significant correlation (Spearman r=0.63,p<0.0001) and an agreement according to Bland-Altman test was identified when comparing all measurements of re-endothelialization in high frequency ultrasound and en face staining. Analysis by animal strain revealed a similar pattern and a significant growth in re-endothelialization length measured in UBM from 2 to 4 weeks could be identified. Immunohistochemical staining for von Willebrand factor confirmed the presence of endothelium in the areas detected as re-endothelialized by the ultrasound assessment. Conclusion: Ultrasound BioMicroscopy can be used for longitudinal in vivo assessment of the re-endothelialization following arterial injury in rats.


2010 ◽  
Vol 16 (S2) ◽  
pp. 1110-1111
Author(s):  
GJ Czarnota

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.


2014 ◽  
Vol 25 (3) ◽  
pp. 176-e45
Author(s):  
Panagiotis Mantis ◽  
Dimitrios Tontis ◽  
David Church ◽  
David Lloyd ◽  
Kim Stevens ◽  
...  

2012 ◽  
Vol 153 (4) ◽  
pp. 632-637.e1 ◽  
Author(s):  
Takashi Kojima ◽  
Sho Yokoyama ◽  
Mayuka Ito ◽  
Rie Horai ◽  
Shuya Hara ◽  
...  

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