A strategy to comprehensively and quickly identify the chemical constituents in Platycodi Radix by ultra‐performance liquid chromatography coupled with traveling wave ion mobility quadrupole time‐of‐flight mass spectrometry

2020 ◽  
Author(s):  
Yang Gao ◽  
Yi Wu ◽  
Shu Liu ◽  
Zhiqiang Liu ◽  
Fengrui Song ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Ion Mobility ◽  
Traveling Wave ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Platycodi Radix ◽  
Traveling Wave Ion Mobility

scholarly journals Rapid Evaluation of Chemical Consistency of Artificially Induced and Natural Resina Draconis Using Ultra-Performance Liquid Chromatography Quadrupole-Time-of-Flight Mass Spectrometry-Based Chemical Profiling

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1850 ◽  
Author(s):  
Qianping Chen ◽  
Lili He ◽  
Changming Mo ◽  
Zhifeng Zhang ◽  
Hairong Long ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Market Demand ◽  
Chemical Profiling ◽  
Flight Mass Spectrometry ◽  
Chromatographic Peaks ◽  
Resina Draconis

Resina Draconis is a highly valued traditional medicine widely used in Arabia since ancient times, and it has been commonly used as an antidiarrheic, antimicrobial, antiulcer, blood circulation promoter as well as an anti-inflammatory agent. The tree source from which this medicine orignates grows extremely slowly, producing a very low yield of Resina Draconis. To meet the increasing market demand, artificial methods for stimulating Resina Draconis formation have been developed and applied. However, the chemical differences between artificially induced Resina Draconis (AIRD) and natural Resina Draconis (NRD) have been rarely studied. The aim of this research was to explore and identify the chemical constituents of AIRD and NRD using ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS) based chemical profiling. A total of 56 chromatographic peaks were detected in AIRD, of these, 44 peaks have had their structures tentatively characterized based on high-resolution mass spectra (HRMS) data, fragmentation ions information, reference standards data and literature review. In total, 40 peaks were found both in AIRD and NRD. The potential chemical transformation mechanisms active in Resina Draconis during formation were explored. To the best of our knowledge, this is the first evaluation of the chemical profiles of both AIRD and NRD. Furthermore, these findings are expected to provide a rational basis for the quality assessment of AIRD and the use of AIRD as a substitute for NRD.

scholarly journals Comparative Analysis of Chemical Constituents of Moringa oleifera Leaves from China and India by Ultra-Performance Liquid Chromatography Coupled with Quadrupole-Time-Of-Flight Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 942 ◽  
Author(s):  
Hongqiang Lin ◽  
Hailin Zhu ◽  
Jing Tan ◽  
Han Wang ◽  
Zhongyao Wang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Moringa Oleifera ◽  
Chemical Constituents ◽  
Time Of Flight ◽  
Ultra Performance Liquid Chromatography ◽  
Multivariate Statistical ◽  
Flight Mass Spectrometry ◽  
Moringa Oleifera Leaves ◽  
Potential Biomarkers

With the aim to discuss the similarities and differences of phytochemicals in Moringa oleifera leaves collected from China (CML) and India (IML) in mind, comparative ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry (UPLC-QTOF-MS) analysis was performed in this study. A screening analysis based on a UNIFI platform was first carried out to discuss the similarities. Next, untargeted metabolomic analysis based on multivariate statistical analysis was performed to discover the differences. As a result, a total of 122 components, containing 118 shared constituents, were characterized from CML and IML. The structure types included flavonoids, alkaloids, glyosides, organic acids and organic acid esters, iridoids, lignans, and steroids, etc. For CML, 121 compounds were characterized; among these, 18 potential biomarkers with higher contents enabled differentiation from IML. For IML, 119 compounds were characterized; among these, 12 potential biomarkers with higher contents enabled differentiation from CML. It could be concluded that both CML and IML are rich in phytochemicals and that CML is similar to IML in the kinds of the compounds it contains, except for the significant differences in the contents of some compounds. This comprehensive phytochemical profile study provides a basis for explaining the effect of different growth environments on secondary metabolites and exists as a reference for further research into or applications of CML in China.

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