Chemometrics‐based models hyphenated with ensemble machine learning for retention time simulation of Isoquercitrin in Coriander sativum L. using high performance liquid chromatography

2020 ◽  
Author(s):  
Abdullahi Garba Usman ◽  
Selin Işik ◽  
Sani Isah Abba ◽  
Filiz Meriçli
Keyword(s):  
Machine Learning ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
High Performance ◽  
Ensemble Machine Learning ◽  
Time Simulation

scholarly journals The Cytology, Isozyme, HPLC Fingerprint, and Interspecific Hybridization Studies of Genus Epimedium (Berberidaceae)

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Lin-Jiao Wang ◽  
Mao-Yin Sheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Interspecific Hybridization ◽  
Retention Time ◽  
High Performance ◽  
Symmetry Type ◽  
Hplc Fingerprint ◽  
C Banding ◽  
Eleven Species

104 samples from 27 accessions belonging to 12 species of genus Epimedium were studied on the basis of cytology observation, POD (i.e., peroxide) isozyme, high performance liquid chromatography (i.e., HPLC) fingerprint, and interspecific hybridization. The cytology observation showed karyotypes of twelve species studied; all are 2A symmetry type of Stebbins standard and similar to each other, and except for karyotype of E. leptorrhizum which is 2n = 2x = 8m (2SAT) + 4sm, the rest are 2n = 2x = 6m (2SAT) + 6sm. Chromosomes C-banding of barrenwort species varies, with 15 to 22 bands, consisting of centromeric bands, intercalary bands, terminal bands, and middle satellite bands. Results of POD isozyme showed that the zymographs vary greatly and sixteen bands were detected in the eleven species, and each species has its own characteristic bands different from the others. Studies on the HPLC fingerprint showed that the HPLC fingerprint of different species has characteristic peaks, divided into two regions (retention time < 10 min and retention time > 10 min). Results of interspecific hybridization showed that crosses of any combination among seven species studied are successful and the rates of grain set vary greatly. Based on these results, the system and phylogeny of this genus were inferred.

Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract

1990 ◽  
Vol 68 (9) ◽  
pp. 1142-1145 ◽  
Author(s):  
Angel Hernanz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Gastrointestinal Tract ◽  
Rat Brain ◽  
Retention Time ◽  
High Performance ◽  
Reverse Phase ◽  
Whole Brain ◽  
Gastrin Releasing Peptide ◽  
Peptide Peak

Extracts of rat brain and gastrointestinal tract, analyzed by reverse-phase high-performance liquid chromatography and radioimmunoassay, contained two bombesin-like immunoreactivity peaks with similar retention times as porcine gastrin-releasing peptide (GRP) and its COOH-terminal decapeptide, neuromedin C or GRP(18–27). However, the GRP-like peptide peak did not elute with exactly the same retention time as porcine GRP. The highest concentration of bombesin-like immunoreactivity was found in extracts of antrum, whereas the lowest was found in whole brain. Neuromedin C was present at lower concentrations than the GRP in antrum, duodenum, and ileum, while similar amounts of each were found in brain.Key words: gastrin-releasing peptide, neuromedin C, rat, brain, intestine.

Corticotrophin-releasing factor immunoreactivity in the hypothalamus of the rat during the perinatal period

1988 ◽  
Vol 119 (1) ◽  
pp. 59-64 ◽  
Author(s):  
A. Chatelain ◽  
F. Boudouresque ◽  
T. Chautard ◽  
J. P. Dupouyt ◽  
C. Oliver
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Median Eminence ◽  
Retention Time ◽  
High Performance ◽  
Perinatal Period ◽  
Adult Rats ◽  
Corticotrophin Releasing Factor ◽  
Specific Radioimmunoassay ◽  
Hypothalamic Control

ABSTRACT Corticotrophin-releasing factor-41 (CRF-41) immunoreactivity has been measured in hypothalamic extracts of fetal (on days 17, 19 and 21 of gestation), neonatal (1, 2, 3 and 4 weeks of age) and adult rats with a specific radioimmunoassay developed for synthetic rat CRF-41. The hypothalamic content (fmol) and concentration (fmol/mg protein) of immunoreactive CRF-41 gradually increased with age. Chromatography of hypothalamic extracts on Sephadex G-50 Fine showed one single peak of immunoreactive CRF-41 which co-eluted with synthetic rat CRF-41. The retention time of hypothalamic CRF-41 during high-performance liquid chromatography was identical to that of synthetic rat CRF-41 at all stages investigated. These results are consistent with the development of neurones containing CRF-41-like molecules in both the hypothalamus and the median eminence of the fetus, as well as with the hypothalamic control of the cortico-stimulating function of the pituitary gland as early as day 19 of gestation. J. Endocr. (1988) 119, 59–64

scholarly journals Senyawa Antibakteri Escherichia coli ATCC 35218 dan Staphylococcus aureus ATCC 25923 dari Kapang Endofit Taman Nasional Gunung Halimun

2012 ◽  
Vol 12 (1) ◽  
pp. 21 ◽  
Author(s):  
Ruth Melliawati ◽  
Harni Harni
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Retention Time ◽  
Endophytic Fungus ◽  
High Performance ◽  
Agar Plate ◽  
Chloroform Fraction ◽  
Antibacterial Compounds

Endophytic fungus is a microorganism which live in the interstitial spaces healthy tissues of the host plant, andhas capability to produce secondary metabolite such as micotoxin, antibiotic and antiviral. This research wasaimed to find out the isolates of endophytic fungus produce antibacterial compounds to inhibit Escerichia coliATCC 35218 and Staphylococcus aureus ATCC 25923, and to investigate the Retention time (Rt) of the antibacterialcompounds produced by endophytic fungus with High Performance Liquid Chromatography (HPLC) methods.Diffusion Agar Plate Method was used to examine the antibacterial compounds on Escerichia coli ATCC 35218 andStaphylococcus aureus ATCC 25923. While the antibacterial compounds were examined with Thin LayerChromatography (TLC) and High Performance Liquid Chromatography (HPLC) methods, and the result werecompared with Chloramphenicol and Ampicillin antibiotic standart. Two isolates of endophytic fungus namelyHl.46F.211 and HL.57F.258 were inhibited the growth Escherichia coli ATCC 35218 and three isolates namelyHL.48F217, HL.53F.243 and HL.57F.258 showed antagonistic action against Staphylococcus aureus ATCC 25923.The results of TLC and HPLC analysing method show that antibacterial compounds produced by endophytic fungusHL.46F.211 had Rt (Retention Time) rate similar with antibiotic Chloramphenicol at 2,796 (at water fraction) and Rtantibiotic Amphycillin at 2,662 (at Chloroform fraction). While antibacterial compounds produced by endophyticfungus HL.57F.258 had Rt rate similar with antibiotic Amphycillin at 2,650 (at Chloroform fraction).

scholarly journals DETECTION AND PURIFICATION OF HARMFUL COMPOUNDS IN BEVERAGES USING HPLC

2016 ◽  
Vol 12 (20) ◽  
pp. 5215-5217 ◽  
Author(s):  
Rukkumani V ◽  
Saravanakumar M
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
Retention Time ◽  
Mobile Phase ◽  
Migration Rate ◽  
High Performance ◽  
Human Beings ◽  
Carcinogenic Effects ◽  
Phase Retention

The presence of harmful compounds like caffeine and carbonated compounds in different beverages like soft drinks, fruit juices deserves great attention because of its toxic and carcinogenic effects on  human beings. We report on the detection and purification of those substances with the help of HPLC(High Performance  Liquid Chromatography).According to the migration rate, stationary phase and mobile phase, retention time we can extract the desire compounds. Depending upon the solvent and sample we can detect the compounds with  the  help  of the detector.The chromatogram will be  displayed and it can be viewed in the PC with the help of  Osiris  software. Compounds like Caffeine, Aspartame, Neotame, Saccharin, Maltodextrin, sucrose, fructose etc can be detected and purified. Detection and purification takes place in the column of HPLC where the process called adsorption takes place. Retention time can be calculated by the total time taken of a component that spends  in both mobile phase and stationary phase. It is always expressed in minutes

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