Supercritical fluid chromatography in chiral separations: Evaluation of equivalency of polysaccharide stationary phases

2020 ◽  
Vol 43 (13) ◽  
pp. 2675-2689 ◽  
Author(s):  
Pavel Jakubec ◽  
Michal Douša ◽  
Lucie Nováková
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Chiral Separations

New insights into supercritical fluid chromatography for chiral separations

2017 ◽  
Vol 9 (17) ◽  
pp. 2603-2610 ◽  
Author(s):  
Hermes Licea-Perez ◽  
Christopher A. Evans
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Chiral Separations ◽  
Chiral Resolution

Supercritical fluid chromatography in combination with chiral stationary phases has proven to be a great tool for chiral resolution, alleviating some of the challenges associated with bioanalysis of stereoisomers.

scholarly journals Development and Validation of Retention Models in Supercritical Fluid Chromatography for Impregnation Process Design

2021 ◽  
Vol 11 (15) ◽  
pp. 7106
Author(s):  
Miaotian Sun ◽  
Zeynep Ülker ◽  
Zhixing Chen ◽  
Sivaraman Deeptanshu ◽  
Monika Johannsen ◽  
...  
Keyword(s):  
Supercritical Fluid ◽  
Supercritical Fluid Chromatography ◽  
Stationary Phases ◽  
Retention Factor ◽  
Retention Mechanism ◽  
Retention Data ◽  
Retention Models ◽  
Retention Factors ◽  
Polar Solutes ◽  
Porous Matrices

The retention factor is the key quantity for the thermodynamic analysis of the retention mechanism in chromatographic experiments. In this work, we measure retention factors for moderately polar solutes on four silica-based porous matrices as stationary phases by supercritical fluid chromatography. Elution of the solutes is only possible with binary mixtures of supercritical carbon dioxide (sc-CO2) and modifier (methanol) due to the low polarity of pure sc-CO2. The addition of modifiers makes the retention mechanism more complex and masks interactions between solute and stationary phase. In this work, we develop and validate several retention models that allow the obtaining of retention factors in modifier-free sc-CO2. Such models pave the way for quantifying adsorption interactions between polar solutes and non-swellable porous matrices in pure sc-CO2 based on retention data obtained in sc-CO2/modifier mixtures. The obtained information will thereby facilitate the understanding and design of impregnation processes, which are often performed in modifier-free conditions.

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