Rapid annotation and structural characterization of saponins in the active fraction of Albizia julibrissin by high‐performance liquid chromatography coupled with quadrupole time of flight mass spectrometry based on accurate mass database

2019 ◽  
Vol 42 (18) ◽  
pp. 2922-2941 ◽  
Author(s):  
Yanfei He ◽  
Tiantian Ni ◽  
Zhaoying Liu ◽  
Yiping Ye ◽  
Hongxiang Sun
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Structural Characterization ◽  
High Performance ◽  
Time Of Flight ◽  
Active Fraction ◽  
Albizia Julibrissin ◽  
Flight Mass Spectrometry

Rapid Detection and Characterization of Steroidal Saponins in the Root of Asparagus cochinchinensis by High-Performance Liquid Chromatography Coupled to Electrospray Ionization and Quadrupole Time-of-Flight Mass Spectrometry

2020 ◽  
Vol 58 (5) ◽  
pp. 454-463
Author(s):  
Yun Ling ◽  
Xin He ◽  
Renliang Jiang ◽  
Qi Zhang ◽  
Shuqin Yuan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
High Performance ◽  
Time Of Flight ◽  
Steroidal Saponins ◽  
Flight Mass Spectrometry ◽  
Asparagus Cochinchinensis

Abstract The dried root of Asparagus cochinchinensis (RAC) has been used as an important traditional Chinese medicine for a long time in China. Steroidal saponins (SSs) are considered to be the main active ingredients of this herb. However, the isolation and structural determination of SSs from RAC are time-consuming and laborious. For this reason, the development of new methods for the separation and characterization of SSs is highly desirable. In this study, a new high-performance liquid chromatography coupled to electrospray ionization and quadrupole time-of-flight mass spectrometry (HPLC-ESI-QTOF-MS/MS) method with precursor ions and the corresponding fragment ions was developed for the identification of SSs in RAC. Finally, 30 SSs have been detected and identified, including 17 potential new compounds. This is the first systematic study of SSs in RAC by HPLC-ESI-QTOF-MS/MS method.

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