Trace enrichment of phenylcarboxylic acids from a model biological fluid and serum of human blood

2019 ◽  
Author(s):  
Alexander Y. Popov ◽  
Zinaida K. Blinnikova ◽  
Maria P. Tsyurupa ◽  
Vadim A. Davankov
Keyword(s):  
Human Blood ◽  
Biological Fluid ◽  
Trace Enrichment ◽  
Phenylcarboxylic Acids

scholarly journals Hemolymph of molluscan origin: from biochemistry to modern biomaterials science

2020 ◽  
Vol 127 (1) ◽  
Author(s):  
Tomasz Machałowski ◽  
Teofil Jesionowski
Keyword(s):  
Immune Response ◽  
Human Blood ◽  
Body Fluid ◽  
Biological Fluid ◽  
Shell Formation ◽  
Future Directions ◽  
Material Chemistry ◽  
Biomineralization Process ◽  
Points Of View ◽  
First Time

AbstractMolluscan hemolymph is a unique kind of body fluid, which in many respects is analogous to human blood, although there are several crucial differences. Here, for the first time, we critically analyze the prospects for applications of this fluid in modern biomaterials science. Particular attention is paid to the biochemistry and chemistry of molluscan hemolymph, as well as to hemocytes and hemocyanins as key functional players within this unique biological fluid. We focus on hemocytes as multifunctional hemolytic cells involved in immune response, and especially in the biomineralization process. The next part of the review contains a discussion of molluscan shell formation and regeneration from different points of view. Finally, we consider the challenges, solutions, and future directions in the application of molluscan hemolymph for bioinspired material chemistry and biomedicine.

scholarly journals An Alternative to the Human Hemoglobin Test in the Investigation of Bloodstains Treated with Active Oxygen: The Human Glycophorin A Test

2011 ◽  
Vol 11 ◽  
pp. 907-916 ◽  
Author(s):  
Ana Castelló ◽  
Francesc Francés ◽  
Fernando Verdú
Keyword(s):  
Human Blood ◽  
Biological Fluid ◽  
Active Oxygen ◽  
Glycophorin A ◽  
Human Hemoglobin ◽  
False Negatives ◽  
Destructive Effect ◽  
Confirmatory Tests ◽  
Three Stages ◽  
Human Glycophorin

In criminal investigations, there are three stages involved when studying bloodstains: search and orientation, confirmation, and individualization. Confirmatory tests have two aims: to show that the stain contains a human biological fluid and to confirm the type of biological fluid. The need to determine the nature of the evidence is reflected in the latest bibliography, where the possibility of employing mRNA and miRNA markers for this purpose is proposed. While these new proposals are being investigated, the kits for determining human hemoglobin currently provide a simple solution for resolving this issue. With these kits, the possibility of obtaining false positives and false negatives is well known. However, recently, a new problem has been detected. This involves the interference caused by new cleaning products that contain sodium percarbonate (or active oxygen) when determining human hemoglobin. With the aim to resolve this problem, this work studied the ability of the human glycophorin A test to determine human blood in samples that have been treated with active oxygen. Our results show that the human glycophorin A test has a greater resistance to the destructive effect of the new detergents containing active oxygen; consequently, it provides an alternative to be taken into consideration in the confirmatory diagnoses of bloodstains.

Thyrotropin-releasing hormone immunoreactivity in human blood, urine, spinal fluid, amniotic fluid, saliva, and gastric juice

1987 ◽  
Vol 114 (4) ◽  
pp. 552-558 ◽  
Author(s):  
Anna-Stina Suhonen-Malm
Keyword(s):  
Amniotic Fluid ◽  
Gastric Juice ◽  
Human Blood ◽  
Biological Fluid ◽  
Biological Fluids ◽  
Exchange Chromatography ◽  
Spinal Fluid ◽  
Reverse Phase ◽  
Blood And Urine Samples ◽  
Radio Immunoassay

Abstract. TRH immunoreactivity levels were measured in human blood, urine, saliva, spinal fluid, amniotic fluid and gastric juice. Urinary TRH excretion during a 48-h period was measured in 11 healthy persons. Blood and urinary TRH immunoreactivity were measured at 2 and 5 h, respectively, after administration of 40 mg of TRH. All the samples were prepurified by SP-Sephadex-C-25 cation-exchange chromatography and subjected to reverse-phase high-pressure liquid chromatography (HPLC). TRH immunoreactivity levels were then measured by our TRH radio-immunoassay. The TRH immunoreactivity (TRH-ir) levels found in urine were 14.6 ± 2.5 pmol/l; in blood 7.5 ± 2.0 pmol/l; in spinal fluid 2.8 ± 1.4 pmol/l, and in gastric juice 23.2 ± 7.1 pmol/l. In all of the amniotic fluid and saliva samples, in almost one half of the blood and spinal fluid samples, and in almost one third of the gastric juice samples, TRH-ir was below the detectable limit. In blood and urine samples taken after oral administration of TRH, TRH-ir was eluted at the same time as synthetic TRH. The recovery of synthetic TRH added to the samples ranged from 36 to 99%. In all of the biological fluid samples, endogenous TRH-ir was eluted at the same time in HPLC, at 15–18 min, as was synthetic TRH which had been added to the samples. Urine was found to contain two TRH immunoreactive peaks, the second of which was eluted at the same time as synthetic TRH. No diurnal variation in urinary TRH excretion or TRH-ir levels was found. The levels of TRH-ir measured in blood, urine, spinal fluid and amniotic fluid are lower than most of those reported earlier. This study suggests that our method can be used to measure TRH immunoreactivity more accurately in a variety of biological fluids.

On the pulsating flow behavior of a biological fluid: human blood

2017 ◽  
Vol 56 (4) ◽  
pp. 387-407 ◽  
Author(s):  
Edtson Emilio Herrera-Valencia ◽  
Fausto Calderas ◽  
Luis Medina-Torres ◽  
Mariano Pérez-Camacho ◽  
Leonardo Moreno ◽  
...  
Keyword(s):  
Human Blood ◽  
Biological Fluid ◽  
Flow Behavior ◽  
Pulsating Flow

Nanogram level quantification of molybdenum(vi) by novel hyphenated SDME/DRS-FTIR in human biological fluid

2015 ◽  
Vol 7 (22) ◽  
pp. 9474-9481 ◽  
Author(s):  
Bhupendra K. Sen ◽  
Swapnil Tiwari ◽  
Manas Kanti Deb ◽  
Shamsh Pervez
Keyword(s):  
Fourier Transform ◽  
Infrared Spectroscopy ◽  
Blood Serum ◽  
Human Blood ◽  
Diffuse Reflectance ◽  
Biological Fluid ◽  
Single Drop ◽  
Ftir Technique ◽  
Nanogram Level ◽  
Serum And Urine

A novel hyphenated single-drop micro-extraction (SDME) diffuse-reflectance Fourier-transform infrared spectroscopy (DRS-FTIR) technique was employed for the quantification of hexavalent molybdenum, i.e., Mo(vi) in human blood serum and urine.

ABSORPTION OF ULTRASOUND IN HUMAN BLOOD

1990 ◽  
Vol 51 (C2) ◽  
pp. C2-311-C2-314
Author(s):  
G. GOPALA KRISHNA ◽  
A. K.W. ANWAR ALI ◽  
A. AHMAD
Keyword(s):  
Human Blood

α-Linoleic acid enhances the capacity of α1-antitrypsin to inhibit lipopolysaccharide-induced IL-1β in human blood neutrophils

Pneumologie ◽  
2016 ◽  
Vol 70 (07) ◽  
Author(s):  
N Aggarwal ◽  
S Wrenger ◽  
V Grau ◽  
T Welte ◽  
S Janciauskiene
Keyword(s):  
Linoleic Acid ◽  
Human Blood ◽  
Blood Neutrophils

Fibrinogen-Fibrin-Related Antigen Pattern in Human Blood

1974 ◽  
Vol 32 (02/03) ◽  
pp. 266-276
Author(s):  
Carl D. Jacobsen ◽  
John C. Hoak ◽  
Kenneth K. WU ◽  
Glenna L. Fry
Keyword(s):  
Human Blood ◽  
Plasma Samples

SummaryIn serum from patients with DIC at least 3 different FR-antigenic components could be found. It was difficult to demonstrate these components in the corresponding plasma samples. It is possible that a portion of these antigens formed as a result of in vitro clotting despite the presence of proteolytic inhibitors. These results suggest that the interpretation of “increased split products in serum” may be more complex than current concepts indicate.

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