Detection of biogenic amines in pet food ingredients by RP-HPLC with automated dansyl chloride derivatization

2018 ◽  
Vol 41 (24) ◽  
pp. 4430-4436 ◽  
Author(s):  
Jessica J. Learey ◽  
Shona Crawford-Clark ◽  
Benjamin J. Bowen ◽  
Colin J. Barrow ◽  
Jacqui L. Adcock
Keyword(s):  
Biogenic Amines ◽  
Dansyl Chloride ◽  
Pet Food ◽  
Food Ingredients ◽  
Rp Hplc

scholarly journals Monitoring the microbiome for food safety and quality using deep shotgun sequencing

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Kristen L. Beck ◽  
Niina Haiminen ◽  
David Chambliss ◽  
Stefan Edlund ◽  
Mark Kunitomi ◽  
...  
Keyword(s):  
Rna Sequencing ◽  
Environmental Changes ◽  
Pet Food ◽  
Analysis Pipeline ◽  
Multiple Sequence ◽  
Food Ingredients ◽  
Total Rna ◽  
Specific Species ◽  
Ingredient Composition ◽  
Food Safety And Quality

AbstractIn this work, we hypothesized that shifts in the food microbiome can be used as an indicator of unexpected contaminants or environmental changes. To test this hypothesis, we sequenced the total RNA of 31 high protein powder (HPP) samples of poultry meal pet food ingredients. We developed a microbiome analysis pipeline employing a key eukaryotic matrix filtering step that improved microbe detection specificity to >99.96% during in silico validation. The pipeline identified 119 microbial genera per HPP sample on average with 65 genera present in all samples. The most abundant of these were Bacteroides, Clostridium, Lactococcus, Aeromonas, and Citrobacter. We also observed shifts in the microbial community corresponding to ingredient composition differences. When comparing culture-based results for Salmonella with total RNA sequencing, we found that Salmonella growth did not correlate with multiple sequence analyses. We conclude that microbiome sequencing is useful to characterize complex food microbial communities, while additional work is required for predicting specific species’ viability from total RNA sequencing.

Salting‐out assisted liquid–liquid extraction with dansyl chloride for the determination of biogenic amines in food

2019 ◽  
Vol 55 (1) ◽  
pp. 248-258 ◽  
Author(s):  
Karen C. A. Francisco ◽  
Pedro F. Brandão ◽  
Rui Miguel Ramos ◽  
Luís Moreira Gonçalves ◽  
Arnaldo A. Cardoso ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Liquid Extraction ◽  
Dansyl Chloride ◽  
Salting Out ◽  
Liquid Liquid Extraction

scholarly journals Biogenic Amines in Traditional Fiore Sardo PDO Sheep Cheese: Assessment, Validation and Application of an RP-HPLC-DAD-UV Method

Separations ◽  
2019 ◽  
Vol 6 (1) ◽  
pp. 11 ◽  
Author(s):  
Claudia Zazzu ◽  
Margherita Addis ◽  
Marco Caredda ◽  
Maria Francesca Scintu ◽  
Giovanni Piredda ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Total Concentration ◽  
Great Variability ◽  
Quantitative Distribution ◽  
Qualitative And Quantitative ◽  
Rp Hplc ◽  
Protected Designation Of Origin ◽  
Assessment Validation ◽  
The Many ◽  
First Time

This contribution aimed to measure for the first time the amount of biogenic amines (BAs) in one of the most ancient and traditional sheep cheese produced in Sardinia, Italy: the Protected Designation of Origin (PDO) Fiore Sardo. To achieve this, an original RP-HPLC-DAD-UV method has been developed that was completely validated in terms of LoD, LoQ, linearity, precision and trueness, and tested on 36 real Fiore Sardo PDO cheese samples produced by four different cheesemakers and marketed by four stores. The average total concentration of the eight BAs (i.e., tyramine, tryptamine, histidine, putrescine, cadaverine, 2-phenylethylamine, spermine and spermidine) measured in Fiore Sardo cheese was 700 mg/kg, with a range between 170 mg/kg and 1,100 mg/kg. A great variability in the total amount of BAs has been evidenced among the Fiore Sardo marketed in the four stores as well as for the cheeses purchased in different times in the same store. Tyramine (350 mg/kg), putrescine (150 mg/kg), histamine (80 mg/kg) and cadaverine (30 mg/kg) are the most abundant BAs found in this matrix. Among the many factors concurring, the dominant microflora of Fiore Sardo PDO is likely the principal cause of the qualitative and quantitative distribution of BAs in this matrix. Finally, the total amount of BAs found in Fiore Sardo PDO is not able to cause any health alert situation for consumers.

scholarly journals Biogenic Amines Determination in “Plant Milks”

Beverages ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 40 ◽  
Author(s):  
Laura Gobbi ◽  
Salvatore Ciano ◽  
Mattia Rapa ◽  
Roberto Ruggieri
Keyword(s):  
Biogenic Amines ◽  
Environmental Issues ◽  
Cow Milk ◽  
Milk Consumption ◽  
Quality And Safety ◽  
Oil Seeds ◽  
Milk Samples ◽  
Rp Hplc ◽  
Water Based ◽  
Specific Regulation

“Plant milks” are water-based beverages, such as, extracts from cereals, pseudo-cereals, oil seeds, legumes or fruits. Plant milk consumption is rising in European and North American markets due to problems related to cow milk allergies, intolerances, but also because of vegan diets and sensitivity to environmental issues. There is no specific regulation for these beverages, therefore their composition can vary considerably, even in the same category. The aim of this study is to characterize the main categories of cereal and pseudo-cereal milks on the market by studying the profile of 8 biogenic amines (histamine, serotonin, spermine, spermidine, putrescine, β-phenylethylamine, cadaverine, tyramine) through a RP-HPLC/FD method with a pre-column derivatization. Biogenic amines are ubiquitous compounds, produced by the decarboxylation of the respective amino acids and they have been proposed as quality and safety markers of different foods and beverages. In the analyzed samples, the total biogenic amines content ranged from a minimum of 1.92 mg/L, to a maximum of 9.27 mg/L. The main biogenic amine found in the samples was histamine. The results show a low content of biogenic amines in all types of analyzed products. This ensures the quality and safety of cereal and pseudo-cereal milk samples.

Dansyl Chloride as a Derivatizing Agent for the Analysis of Biogenic Amines by CZE-UV

2020 ◽  
Vol 83 (6) ◽  
pp. 767-778 ◽  
Author(s):  
Jéssica Oliveira Fernandes Mantoanelli ◽  
Luís Moreira Gonçalves ◽  
Elisabete Alves Pereira
Keyword(s):  
Biogenic Amines ◽  
Dansyl Chloride ◽  
Derivatizing Agent

Determination of biogenic amines by RP-HPLC of the dabsyl derivates

1996 ◽  
Vol 203 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Annette Bockhardt ◽  
Ingolf Krause ◽  
Henning Klostermeyer
Keyword(s):  
Biogenic Amines ◽  
Rp Hplc

scholarly journals Development and validation of RP-HPLC-DAD method for biogenic amines determination in probiotic yogurts

2020 ◽  
Vol 13 (1) ◽  
pp. 1582-1597 ◽  
Author(s):  
Carla Paulo Vieira ◽  
Marion Pereira da Costa ◽  
Vitor L.M. Silva ◽  
Beatriz da Silva Frasao ◽  
Letícia F.M. Campos de Aquino ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Rp Hplc ◽  
Development And Validation

Simultaneous detection of fifteen biogenic amines in animal derived products by HPLC-FLD with solid-phase extraction after derivatization with dansyl chloride

2016 ◽  
Vol 8 (18) ◽  
pp. 3747-3755 ◽  
Author(s):  
Huaping Zhu ◽  
Shanshan Yang ◽  
Yan Zhang ◽  
Guozhen Fang ◽  
Shuo Wang
Keyword(s):  
Solid Phase Extraction ◽  
Biogenic Amines ◽  
Solid Phase ◽  
Simultaneous Detection ◽  
Dansyl Chloride ◽  
Phase Extraction

After extraction, derivatization and purification, fifteen kinds of biogenic amines in meat were separated and quantitated by HPLC-FLD.

scholarly journals Bacteriophages safely reduceSalmonellacontamination in pet food and raw pet food ingredients

Bacteriophage ◽  
2016 ◽  
Vol 6 (3) ◽  
pp. e1220347 ◽  
Author(s):  
Nitzan Soffer ◽  
Tamar Abuladze ◽  
Joelle Woolston ◽  
Manrong Li ◽  
Leigh Farris Hanna ◽  
...  
Keyword(s):  
Pet Food ◽  
Food Ingredients
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