Chemical profiling of bioactive constituents in hop cones and pellets extracts by online comprehensive two-dimensional liquid chromatography with tandem mass spectrometry and direct infusion Fourier transform ion cyclotron resonance mass spectrometry

2018 ◽  
Vol 41 (7) ◽  
pp. 1548-1557 ◽  
Author(s):  
Eduardo Sommella ◽  
Francesco Pagano ◽  
Emanuela Salviati ◽  
Marcello Chieppa ◽  
Alessia Bertamino ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Liquid Chromatography ◽  
Cyclotron Resonance ◽  
Ion Cyclotron Resonance ◽  
Tandem Mass ◽  
Two Dimensional ◽  
Direct Infusion ◽  
Bioactive Constituents ◽  
Chemical Profiling

scholarly journals Advanced Mass Spectrometric Methods for the Rapid and Quantitative Characterization of Proteomes

2002 ◽  
Vol 3 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Richard D. Smith
Keyword(s):  
Mass Spectrometry ◽  
Fourier Transform ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Tandem Mass Spectrometry ◽  
Cyclotron Resonance ◽  
High Sensitivity ◽  
Accurate Mass ◽  
Ion Cyclotron Resonance ◽  
Tandem Mass

Progress is reviewed towards the development of a global strategy that aims to extend the sensitivity, dynamic range, comprehensiveness and throughput of proteomic measurements based upon the use of high performance separations and mass spectrometry. The approach uses high accuracy mass measurements from Fourier transform ion cyclotron resonance mass spectrometry (FTICR) to validate peptide ‘accurate mass tags’ (AMTs) produced by global protein enzymatic digestions for a specific organism, tissue or cell type from ‘potential mass tags’ tentatively identified using conventional tandem mass spectrometry (MS/MS). This provides the basis for subsequent measurements without the need for MS/ MS. High resolution capillary liquid chromatography separations combined with high sensitivity, and high resolution accurate FTICR measurements are shown to be capable of characterizing peptide mixtures of more than105components. The strategy has been initially demonstrated using the microorganismsSaccharomyces cerevisiaeandDeinococcus radiodurans.Advantages of the approach include the high confidence of protein identification, its broad proteome coverage, high sensitivity, and the capability for stableisotope labeling methods for precise relative protein abundance measurements.Abbreviations: LC, liquid chromatography; FTICR, Fourier transform ion cyclotron resonance; AMT, accurate mass tag; PMT, potential mass tag; MMA, mass measurement accuracy; MS, mass spectrometry; MS/MS, tandem mass spectrometry; ppm, parts per million.

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