Chromatographic efficiency of polar capillary columns applied for the analysis of fatty acid methyl esters by gas chromatography

2018 ◽  
Vol 41 (7) ◽  
pp. 1582-1592 ◽  
Author(s):  
Habtewold D. Waktola ◽  
Svein A. Mjøs
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Capillary Columns ◽  
Acid Methyl

Tutorial for the Characterization of Fatty Acid Methyl Esters by Gas Chromatography with Highly Polar Capillary Columns

2020 ◽  
Author(s):  
Pierluigi Delmonte ◽  
Andrea Milani ◽  
John K G Kramer
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Vegetable Oils ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Capillary Columns ◽  
Marine Oils ◽  
Optimal Separation ◽  
Acid Methyl

Abstract The fatty acid composition of fats and oils is commonly determined by gas chromatography after preparing fatty acid methyl esters (FAME). Capillary columns coated with polyethylene glycol emerged as the preferred separation tool for the quantification of the polyunsaturated fatty acids contained primarily in marine oils. However, their selectivity is inadequate for measuring the trans fatty acids (TFA) contained in refined vegetable oils, dairy fats, and marine oils. Highly polar 100% poly(biscyanopropyl siloxane) capillary columns provide the necessary selectivity, but small differences in the phase polarity caused by column age, conditioning, or manufacturing variations affect the reproducibility of their separations of these complex samples. In this study, a simple procedure is described to compensate for small variations in column selectivity by adjusting the elution temperature. The balance between the dipole-induced dipole interactions and dispersive interactions was determined by measuring selectivity factors [SF(i)] corresponding to the elution of an unsaturated FAME such as 18:3n-3 relative to two saturated FAME such as 20:0 and 22:0. Knowing the SF(i) provided by the installed capillary column at a given elution temperature, and the SF(i) of the target separation, we propose a simple calculation to determine the necessary elution temperature adjustment to achieve (or restore) the desired separation. After determining the SF(i) which provides the optimal separation of TFA, the novel methodology was applied to the separation of refined vegetable oils, butter fats, and marine oils.

On the determination of the distillation curve of fatty acid methyl esters by gas chromatography

Fuel ◽  
2022 ◽  
Vol 314 ◽  
pp. 123143
Author(s):  
Rodica Niculescu ◽  
Mihaela Năstase ◽  
Adrian Clenci
Keyword(s):  
Gas Chromatography ◽  
Fatty Acid ◽  
Fatty Acid Methyl Esters ◽  
Methyl Esters ◽  
Distillation Curve ◽  
Acid Methyl
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