Two new oxygen-containing biomarkers isolated from the Chinese Maoming oil shale by silica gel column chromatography and preparative gas chromatography

2017 ◽  
Vol 40 (3) ◽  
pp. 813-818 ◽  
Author(s):  
Xiangyun Zhang ◽  
Hong Lu ◽  
Jing Liao ◽  
Caiming Tang ◽  
Guoying Sheng ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Silica Gel ◽  
Column Chromatography ◽  
Oil Shale ◽  
Preparative Gas Chromatography

scholarly journals Identification of the AntiListerialConstituents in Partially Purified Column Chromatography Fractions ofGarcinia kolaSeeds and Their Interactions with Standard Antibiotics

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
D. Penduka ◽  
L. Buwa ◽  
B. Mayekiso ◽  
A. K. Basson ◽  
A. I. Okoh
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Silica Gel ◽  
Column Chromatography ◽  
Mobile Phase ◽  
Hexane Extract ◽  
The Other ◽  
Penicillin G ◽  
Garcinia Kola ◽  
Maximum Test

Partially purified fractions of the n-hexane extract ofGarcinia kolaseeds were obtained through column chromatography and their constituents were identified through the use of gas chromatography coupled to mass spectrometry (GC-MS). Three fractions were obtained by elution with benzene as the mobile phase and silica gel 60 as the stationery phase and these were named Benz1, Benz2, and Benz3 in the order of their elution. The antiListerialactivities of these fractions were assessed through MIC determination and only Benz2 and Benz3 were found to be active with MIC’s ranging from 0.625 to 2.5 mg/mL. The results of the GC-MS analysis showed Benz2 to have 9 compounds whilst Benz3 had 7 compounds, with the major compounds in both fractions being 9,19-Cyclolanost-24-en-3-ol, (3.β.) and 9,19-Cyclolanostan-3-ol,24-methylene-, (3.β.). The Benz2 fraction was found to have mainly indifferent interactions with ampicillin and penicillin G whilst mainly additive interactions were observed with ciprofloxacin. The Benz3 fraction’s interactions were found to be 50% synergistic with penicillin G and 25% synergistic with ciprofloxacin and ampicillin. A commercially available 9,19-Cyclolanost-24-en-3-ol, (3.β.) was found not to exhibit any antiListerialactivities at maximum test concentrations of 5 mg/mL, suggesting that the compound could be acting in synergy with the other compounds in the eluted fractions ofGarcinia kolaseeds.

Chemical Constituents of the Essential Oils of Aroliaceae and Lauraceae Families

1975 ◽  
Vol 30 (5-6) ◽  
pp. 421-421
Author(s):  
Nanao Hayashi ◽  
Kôki Yokochyô ◽  
Hisashi Komae
Keyword(s):  
Gas Chromatography ◽  
Essential Oils ◽  
Column Chromatography ◽  
Infrared Spectra ◽  
Steam Distillation ◽  
Chemical Constituents ◽  
Preparative Gas Chromatography ◽  
The Individual

Abstract In the course of the chemosystematic study of Aroliaceae and Lauraceae families, the leaf oils of some plants were investigated. The leaf oils were obtained by steam distillation of the fresh leaves. The individual constituents were isolated by column chromatography followed by preparative gas chromatography, and identified by gas chromatography and by infrared spectra.

scholarly journals Study on Lavender Essential Oil Chemical Compositions by GC-MS and Improved pGC

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3166 ◽  
Author(s):  
Guangyao Dong ◽  
Xiaohui Bai ◽  
Aoken Aimila ◽  
Haji Aisa ◽  
Maitinuer Maiwulanjiang
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Column Chromatography ◽  
Capillary Columns ◽  
Chemical Compositions ◽  
Lavandula Angustifolia ◽  
Aerial Parts ◽  
Preparative Gas Chromatography ◽  
The Cost ◽  
First Time

Lavender essential oil from the aerial parts of Lavandula angustifolia Mill. was analyzed by GC-MS equipped with three capillary columns of different polarities, which were HP-1, HP-5 ms and HP-INNOWax. A total of 40 compounds were identified by GC-MS, accounting for 92.03% of the total essential oil compositions. Nineteen monomers were separated by column chromatography and improved preparative gas chromatography (pGC), six of which could not be retrieved from the NIST 14 (National Institute of Standards and Technology, USA; 14th edition) library database. Fifteen compounds were identified for the first time in lavender essential oil. The improved pGC not only doubled the efficiency but also greatly reduced the cost.

scholarly journals The Examination of Semi-Volatiles in Cigarette Smoke/Untersuchung der „Semi -Volatiles“ des Cigarettenrauches

1971 ◽  
Vol 6 (1) ◽  
Author(s):  
G. Neurath ◽  
M. Dünger ◽  
I. Küstermann
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cigarette Smoke ◽  
Silica Gel ◽  
Column Chromatography ◽  
Tobacco Smoke ◽  
Tobacco Smoke Condensate

AbstractSix fractions of the steam volatiles of tobacco smoke condensate (semi-volatiles) are formed by column chromatography on silica gel. The constituents are identified by means of a combination of gas chromatography and mass spectrometry. About 320 compounds are indicated. Out of the 215 substances identified 69 have not been previously reported in cigarette smoke.

A Cytotoxic Natural Product from Patrinia villosa Juss

2019 ◽  
Vol 19 (11) ◽  
pp. 1399-1404 ◽  
Author(s):  
Yangcheng Liu ◽  
Wei Liu ◽  
Changlan Chen ◽  
Zheng Xiang ◽  
Hongwei Liu
Keyword(s):  
Cell Cycle ◽  
Cell Lines ◽  
Silica Gel ◽  
Column Chromatography ◽  
Bioactive Compound ◽  
Antitumor Agent ◽  
2D Nmr ◽  
Preparative Hplc ◽  
Dependent Manner ◽  
2D Nmr Spectra

Background and Purpose:: Patrinia villosa Juss is an important Chinese herbal medicine widely used for thousands of years, but few reports on the ingredients of the herb have been presented. In this study, we aim to isolate the bioactive compound from the plant. Material and Methods:: The air-dried leaves of P. villosa (15kg) were extracted three times with 70% EtOH under reflux. The condensed extract was suspended in H2O and partitioned with light petroleum, dichloromethane and n-BuOH. The dichloromethane portion was then subjected to normal-phase silica gel column chromatography, ODS silica gel column chromatography and semi-preparative HPLC to yield compound 1. Cytotoxicities of 1 were assayed on HepG2, A549 and A2780 cell lines. The mechanism of apoptosis and cell cycle on A549 was confirmed subsequently. Results: A new impecylone (Impecylone A) was isolated from the leaves of Patrinia villosa Juss, and its structures were established using 1D, 2D-NMR spectra and HR-ESI-MS. Impecylone A could selectivity inhibit HepG2 and A549 cell lines. The compound could induce apoptosis of A549 and arrest the cell cycle at G2/M phase in a dose-dependent manner. Conclusion: Impecylone A is a novel compound from Patrinia villosa Juss and could be a potential antitumor agent especially in the cell lines of A549.

scholarly journals Separation of lipids by Silica Gel G column chromatography

1964 ◽  
Vol 5 (3) ◽  
pp. 479-481
Author(s):  
Quincy E. Crider ◽  
Petar Alaupovic ◽  
Joe Hillsberry ◽  
Cathy Yen ◽  
Reagan H. Bradford
Keyword(s):  
Silica Gel ◽  
Column Chromatography

scholarly journals Anti-Neuroinflammatory and Anti-Inflammatory Activities of Phenylheptatriyne Isolated from the Flowers of Coreopsis lanceolata L. via NF-κB Inhibition and HO-1 Expression in BV2 and RAW264.7 Cells

2021 ◽  
Vol 22 (14) ◽  
pp. 7482
Author(s):  
Hwan Lee ◽  
Zhiming Liu ◽  
Chi-Su Yoon ◽  
Linsha Dong ◽  
Wonmin Ko ◽  
...  
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Raw264.7 Cells ◽  
Necrosis Factor Alpha ◽  
Tnf Α ◽  
Etoac Fraction ◽  
Factor Alpha ◽  
Anti Inflammatory ◽  
And Oxidative Stress ◽  
Necrosis Factor

Aging is associated with immune disregulation and oxidative stress which lead to inflammation and neurodegenerative diseases. We have tried to identify the anti-neuroinflammatory and anti-inflammatory components of Coreopsis lanceolata L. The dried flowers of C. lanceolata were extracted with 70% EtOH, and the obtained extract was divided into CH2Cl2, EtOAc, n-BuOH, and H2O fractions. The CH2Cl2 fraction was separated using silica gel and C-18 column chromatography to yield phenylheptatriyne (1), 2′-hydroxy-3,4,4′-trimethoxychalcone (2), and 4′,7-dimethoxyflavanone (3). Additionally, the EtOAc fraction was subjected to silica gel, C-18, and Sephadex LH-20 column chromatography to yield 8-methoxybutin (4) and leptosidin (5). All the compounds isolated from C. lanceolata inhibited the production of nitric oxide (NO) in LPS-induced BV2 and RAW264.7 cells. In addition, phenylheptatriyne and 4′,7-dimethoxyflavanone reduced the secretion of inflammatory cytokines, tumor necrosis factor alpha (TNF-α), and interleukin (IL)-6. Among them, phenylheptatriyne was significantly downregulated in the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2). Subsequently, phenylheptatriyne also effectively inhibited nuclear factor-kappa B (NF-κB) activation in LPS-stimulated BV2 and RAW264.7 cells. Based on these results, the anti-neuroinflammatory effect of phenylheptatriyne isolated from C. lanceolata was confirmed, which may exert a therapeutic effect in treatment of neuroinflammation-related diseases.

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