Simultaneous analysis of ten low-molecular-mass organic acids in the tricarboxylic acid cycle and photorespiration pathway inThalassiosira pseudonanaat different growth stages

2016 ◽  
Vol 40 (3) ◽  
pp. 635-645 ◽  
Author(s):  
Mengwei Ye ◽  
Lijing Zhang ◽  
Panpan Xu ◽  
Runtao Zhang ◽  
Jilin Xu ◽  
...  
Keyword(s):  
Organic Acids ◽  
Molecular Mass ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Simultaneous Analysis ◽  
Growth Stages ◽  
Acid Cycle ◽  
Different Growth Stages

scholarly journals New conditions of HPLC analysis for separation and quantification of simple organic acids of tricarboxylic acid cycle in psoriasis

2020 ◽  
Author(s):  
Jiaxing Fan ◽  
Yiyang Liu ◽  
Shenmin Su ◽  
Yiqing Cao ◽  
Yunqiu Yu
Keyword(s):  
Organic Acids ◽  
High Performance ◽  
Aldol Condensation ◽  
Tricarboxylic Acid Cycle ◽  
Simultaneous Detection ◽  
Tricarboxylic Acid ◽  
Acid Cycle ◽  
Physiological Processes ◽  
Aconitic Acid ◽  
Important Pathway

AbstractTricarboxylic acid cycle is an important pathway and the metabolites of tricarboxylic acid cycle, simple organic acids, play important roles in many physiological processes. The new conditions of high performance liquid chromatography (HPLC) with improved separation selectivity were developed and optimized by choosing suitable store solvent, methanol, which can catalyze the reaction, aldol condensation, and facilitate the formation of new compound to improve simultaneous detection of nine metabolites of tricarboxylic acid cycle, including lactic acid, pyruvic acid, citric acid, alpha-ketoglutaric acid, malic acid, succinic acid, cis-aconitic acid, itaconic acid, and fumaric acid, and be applied in analysis of the nine metabolites in psoriasis mice skin. This study shows that in process of developing methods, expect conventional chromatographic condition, solvent should also be considered carefully.

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