Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Terence Hetzel; Denise Loeker; Thorsten Teutenberg; Torsten C. Schmidt

doi:10.1002/jssc.201600775

Characterization of the efficiency of microbore liquid chromatography columns by van Deemter and kinetic plot analysis

Journal of Separation Science ◽

10.1002/jssc.201600775 ◽

2016 ◽

Vol 39 (20) ◽

pp. 3889-3897 ◽

Cited By ~ 6

Author(s):

Terence Hetzel ◽

Denise Loeker ◽

Thorsten Teutenberg ◽

Torsten C. Schmidt

Keyword(s):

Liquid Chromatography ◽

Plot Analysis ◽

Chromatography Columns ◽

Microbore Liquid Chromatography

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Characterization of peak capacity of microbore liquid chromatography columns using gradient kinetic plots

Journal of Chromatography A ◽

10.1016/j.chroma.2017.01.018 ◽

2017 ◽

Vol 1485 ◽

pp. 62-69 ◽

Cited By ~ 4

Author(s):

Terence Hetzel ◽

Christina Blaesing ◽

Martin Jaeger ◽

Thorsten Teutenberg ◽

Torsten C. Schmidt

Keyword(s):

Liquid Chromatography ◽

Peak Capacity ◽

Kinetic Plots ◽

Chromatography Columns ◽

Microbore Liquid Chromatography

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The influence of injection volume on efficiency of microbore liquid chromatography columns for gradient and isocratic elution

Journal of Chromatography A ◽

10.1016/j.chroma.2021.461965 ◽

2021 ◽

Vol 1641 ◽

pp. 461965

Author(s):

Tobias Werres ◽

Torsten C. Schmidt ◽

Thorsten Teutenberg

Keyword(s):

Liquid Chromatography ◽

Isocratic Elution ◽

Injection Volume ◽

Chromatography Columns ◽

Microbore Liquid Chromatography

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Rapid and selective characterization of influenza virus constituents in monovalent and multivalent preparations using non-porous reversed-phase high performance liquid chromatography columns

Journal of Chromatography A ◽

10.1016/j.chroma.2006.04.003 ◽

2006 ◽

Vol 1123 (2) ◽

pp. 225-232 ◽

Cited By ~ 33

Author(s):

Virginia Garcia-Cañas ◽

Barry Lorbetskie ◽

Michel Girard

Keyword(s):

High Performance Liquid Chromatography ◽

Influenza Virus ◽

Liquid Chromatography ◽

High Performance ◽

Reversed Phase ◽

Chromatography Columns

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Characterization of High‐Pressure Liquid Chromatography Columns using Chromatographic Methods

Analytical Letters ◽

10.1080/00032710600773424 ◽

2006 ◽

Vol 39 (10) ◽

pp. 2095-2152 ◽

Cited By ~ 15

Author(s):

Pavel Jandera ◽

Kateřina Novotná

Keyword(s):

High Pressure ◽

Liquid Chromatography ◽

High Pressure Liquid Chromatography ◽

Chromatographic Methods ◽

Chromatography Columns

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Comparative characterization of hydrophilic interaction liquid chromatography columns by linear solvation energy relationships

Journal of Chromatography A ◽

10.1016/j.chroma.2012.11.075 ◽

2013 ◽

Vol 1273 ◽

pp. 73-94 ◽

Cited By ~ 59

Author(s):

Georg Schuster ◽

Wolfgang Lindner

Keyword(s):

Liquid Chromatography ◽

Solvation Energy ◽

Hydrophilic Interaction Liquid Chromatography ◽

Linear Solvation Energy Relationships ◽

Hydrophilic Interaction ◽

Energy Relationships ◽

Linear Solvation Energy ◽

Chromatography Columns ◽

Linear Solvation

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Characterization of reversed-phase liquid chromatography columns with retention indexes of standards based on an alkyl aryl ketone scale

Analytical Chemistry ◽

10.1021/ac00266a031 ◽

1984 ◽

Vol 56 (2) ◽

pp. 256-262 ◽

Cited By ~ 69

Author(s):

Roger M. Smith

Keyword(s):

Liquid Chromatography ◽

Reversed Phase ◽

Reversed Phase Liquid Chromatography ◽

Alkyl Aryl ◽

Alkyl Aryl Ketone ◽

Phase Liquid ◽

Chromatography Columns ◽

Retention Indexes ◽

Aryl Ketone

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Characterization of protein iv-glycosylation by reversed-phase microbore liquid chromatography / electrospray mass spectrometry, complementary mobile phases, and sequential exoglycosidase digestion

Journal of the American Society for Mass Spectrometry ◽

10.1016/1044-0305(94)85050-x ◽

1994 ◽

Vol 5 (5) ◽

pp. 350-358 ◽

Cited By ~ 51

Author(s):

K. F. Medzihradszky ◽

D. A. Maltby ◽

S. C. Hall ◽

C. A. Settineri ◽

A. L. Burlingame

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

Electrospray Mass Spectrometry ◽

Reversed Phase ◽

Mobile Phases ◽

Microbore Liquid Chromatography

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Characterization of retentivity of reversed phase liquid chromatography columns

Talanta ◽

10.1016/0039-9140(91)80042-x ◽

1991 ◽

Vol 38 (3) ◽

pp. 237-243 ◽

Cited By ~ 24

Author(s):

P YING ◽

J DORSEY

Keyword(s):

Liquid Chromatography ◽

Reversed Phase ◽

Reversed Phase Liquid Chromatography ◽

Phase Liquid ◽

Chromatography Columns

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Characterization of warfarin unusual peak profiles on oligoproline chiral high performance liquid chromatography columns

Journal of Chromatography A ◽

10.1016/j.chroma.2010.08.066 ◽

2010 ◽

Vol 1217 (42) ◽

pp. 6545-6554 ◽

Cited By ~ 10

Author(s):

Wenjian Lao ◽

Jay Gan

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Chromatography Columns

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Purification and characterization of activated human erythrocyte prolidase

Biochemistry and Cell Biology ◽

10.1139/o89-005 ◽

1989 ◽

Vol 67 (1) ◽

pp. 34-41 ◽

Cited By ~ 22

Author(s):

Andrea M. Richter ◽

Gerald L. Lancaster ◽

Francis Y. M. Choy ◽

Peter Hechtman

Keyword(s):

Molecular Weight ◽

Liquid Chromatography ◽

Human Kidney ◽

Human Erythrocytes ◽

Rabbit Antibody ◽

Purification And Characterization ◽

Sds Page ◽

Erythrocyte Enzyme ◽

Chromatography Columns

Prolidase (E.C. 3.4.13.9) has been purified 7500-fold to homogeneity from human erythrocytes in a Mn2+-activated form using conventional and fast protein liquid chromatography columns. The procedure includes a 1-h incubation of the crude hemolysate at 50 °C with 1 mM MnCl2. Following this novel step, prolidase retains full activity, obviating the requirement for preincubation of each enzyme fraction with Mn2+ prior to assay. Preincubation with MnCl2 does not change the isoelectric point of the enzyme. The molecular weight of the purified enzyme was 58 000 when measured by SDS–PAGE. Western blotting, using rabbit antibody raised to human kidney prolidase, with partially purified erythrocyte enzyme revealed a cross-reacting band at Mr 58 000.Key words: prolidase purification, human erythrocytes.

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