Structural characterization and identification of flavonoid aglycones in threeGlycyrrhizaspecies by liquid chromatography with photodiode array detection and quadrupole time-of-flight mass spectrometry

2016 ◽  
Vol 39 (11) ◽  
pp. 2068-2078 ◽  
Author(s):  
Shiqi Fang ◽  
Qiyang Qu ◽  
Yunfeng Zheng ◽  
Huanhuan Zhong ◽  
Chenxiao Shan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Structural Characterization ◽  
Time Of Flight ◽  
Photodiode Array Detection ◽  
Flavonoid Aglycones ◽  
Flight Mass Spectrometry ◽  
Photodiode Array ◽  
Array Detection

scholarly journals Simultaneous Determination of 12 Marker Components in Yeonkyopaedok-san Using HPLC–PDA and LC–MS/MS

2020 ◽  
Vol 10 (5) ◽  
pp. 1713 ◽  
Author(s):  
Chang-Seob Seo ◽  
Hyeun-Kyoo Shin
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Formic Acid ◽  
Coefficient Of Determination ◽  
Photodiode Array Detection ◽  
Mobile Phases ◽  
Photodiode Array ◽  
Array Detection ◽  
Aqueous Formic Acid

Yeonkyopaedok-san is a traditional Korean medicine used in the early treatment of boils. In the present study, its 12 marker components for quality control were determined using high-performance liquid chromatography (HPLC) with photodiode array detection and ultra-performance liquid chromatography–mass spectrometry with tandem mass spectrometry (UPLC–MS/MS). The investigated 12 marker components of Yeonkyopaedok-san were as follows: 3-caffeoylquinic acid, cimifugin 7-glucoside, liquiritin apioside, ferulic acid, narirutin, 5-O-methylvisammioside, naringin, neohesperidin, oxypeucedanin hydrate, arctigenin, glycyrrhizic acid, and 6-gingerol. The analytical column used for the separation of the 12 marker analytes in Yeonkyopaedok-san was a Waters SunFire C18 column (4.6 mm × 250 mm, 5 μm). The two mobile phases used were 0.1% (v/v) aqueous formic acid and 0.1% (v/v) formic acid in acetonitrile. In the UPLC–MS/MS analysis, all components were separated using a Waters ACQUITY UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm). The two mobile phases used were 0.1% (v/v) aqueous formic acid and acetonitrile. The coefficient of determination of the calibration curves in both analysis systems showed good linearity (>0.99). The amounts of the 12 marker components in Yeonkyopaedok-san determined using HPLC–photodiode array detection and UPLC–MS/MS analyses were found to be 0.14–9.00 mg/g and 2.35–853.11 μg/g, respectively.

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