Analysis of nitromethane from samples exposed in vitro to chloropicrin by stable isotope dilution headspace gas chromatography with mass spectrometry

2015 ◽  
Vol 38 (19) ◽  
pp. 3383-3389 ◽  
Author(s):  
Mia Halme ◽  
Maija Pesonen ◽  
Toni Grandell ◽  
Matti Kuula ◽  
Markku Pasanen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Headspace Gas Chromatography ◽  
Stable Isotope Dilution ◽  
Headspace Gas

scholarly journals Methylmalonic acid quantification by stable isotope dilution gas chromatography-mass spectrometry from filter paper urine samples

1996 ◽  
Vol 42 (6) ◽  
pp. 910-914 ◽  
Author(s):  
M T McCann ◽  
M M Thompson ◽  
I C Gueron ◽  
B Lemieux ◽  
R Giguère ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Stable Isotope ◽  
Isotope Dilution ◽  
Filter Paper ◽  
Methylmalonic Acid ◽  
Urine Samples ◽  
Gas Chromatography Mass Spectrometry ◽  
Urinary Creatinine ◽  
Stable Isotope Dilution

Abstract A specific method for quantification of methylmalonic acid (MMA) from urine samples dried onto filter paper is described. The method involves stable isotope dilution gas chromatography-mass spectrometry with [methyl-2H3]-MMA as the internal standard. MMA is stable in dry paper samples stored at room temperature for at least 2 weeks. The extraction efficiency of MMA from paper was 56-58%. The concentration of urinary MMA in dried filter paper specimens from 190 normal controls was 1.21 +/- 1.34 (mean +/- SD) mmol/mol of urinary creatinine. Age-related reference values are also reported. The concentrations, normalized to the urinary creatinine concentration, decrease with age. The applicability of this method to rapid screening for cobalamin (vitamin B12)-related disorders and methylmalonic aciduria is demonstrated.

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