Preconcentration of indapamide from human urine using molecularly imprinted solid-phase extraction

2015 ◽  
Vol 38 (17) ◽  
pp. 3090-3095 ◽  
Author(s):  
Hüma Yılmaz ◽  
Hasan Basan
Keyword(s):  
Solid Phase Extraction ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Molecularly Imprinted

Novel restricted access material combined to molecularly imprinted polymer for selective magnetic solid-phase extraction of estrogens from human urine

2019 ◽  
Vol 149 ◽  
pp. 104043 ◽  
Author(s):  
Hanna Leijoto de Oliveira ◽  
Bruna Carneiro Pires ◽  
Leila Suleimara Teixeira ◽  
Laíse Aparecida Fonseca Dinali ◽  
Nathália Soares Simões ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Magnetic Solid Phase Extraction ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Restricted Access Material ◽  
Magnetic Solid

Solid-phase extraction using molecularly imprinted polymer for determination of ochratoxin A in human urine

2015 ◽  
Vol 8 (1) ◽  
pp. 37-43 ◽  
Author(s):  
L. Xie ◽  
P. Sheng ◽  
W. Kong ◽  
X. Zhao ◽  
Z. Ou-Yang ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Solid Phase Extraction ◽  
Ochratoxin A ◽  
Molecularly Imprinted Polymer ◽  
Human Urine ◽  
Solid Phase ◽  
Phase Extraction ◽  
Imprinted Polymer ◽  
Molecularly Imprinted

A rapid, selective and reliable sample preparation technique employing solid-phase extraction (SPE) based on molecularly imprinted polymer (MIP) for the determination of ochratoxin A (OTA) in human urine was described. After sample adjustment to pH 2.5 with 0.1 M HCl, the urine sample was loaded onto the MIP-SPE column, and after a wash step, OTA was eluted for measurement by ultra-high performance liquid chromatography coupled with fluorescence detection. Key parameters which affected the MIP-SPE extraction efficiency were optimized as was the detection method. Under the optimised conditions, the limits of detection and quantification for OTA in urine were 0.2 ng/ml and 0.6 ng/ml, respectively. The recoveries for OTA in urine, spiked at the 0.6, 6.0 and 60 ng/ml levels, ranged from 92.0 to 98.9%. Sixty urine samples were analysed, of which four were found to contain OTA at concentrations ranging from 0.022 to 0.083 ng/ml; the positive results were confirmed by liquid chromatography coupled with tandem mass spectrometry. OTA determination in urine is a good indicator for human exposure to the mycotoxin, and this is the first report on OTA contamination in Chinese people.

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