Purification of papain by metal affinity partitioning in aqueous two-phase polyethylene glycol/sodium sulfate systems

2015 ◽  
Vol 38 (8) ◽  
pp. 1426-1432 ◽  
Author(s):  
Zhi-Guo Jiang ◽  
Hai-De Zhang ◽  
Wei-Tao Wang
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Sulfate ◽  
Two Phase ◽  
Metal Affinity ◽  
Affinity Partitioning

scholarly journals Origin of salt additive effect on solute partitioning in aqueous polyethylene glycol-8000–sodium sulfate two-phase system

2014 ◽  
Vol 1337 ◽  
pp. 3-8 ◽  
Author(s):  
Nuno R. da Silva ◽  
Luisa A. Ferreira ◽  
Larissa M. Mikheeva ◽  
José A. Teixeira ◽  
Boris Y. Zaslavsky
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Sulfate ◽  
Additive Effect ◽  
Phase System ◽  
Two Phase ◽  
Solute Partitioning

scholarly journals Interphase distribution of a number of polybasic carboxylic acids in aqueous two-phase systems based on polyethylene glycol-1500

2022 ◽  
Vol 1212 (1) ◽  
pp. 012022
Author(s):  
S V Solov’eva ◽  
V O Solov’ev ◽  
I V Zinov’eva
Keyword(s):  
Polyethylene Glycol ◽  
Carboxylic Acids ◽  
Sodium Sulfate ◽  
Aqueous Systems ◽  
Two Phase ◽  
Interphase Distribution ◽  
Tartaric Acids ◽  
Quantitative Characteristics ◽  
Aqueous Two Phase Systems ◽  
Polyethylene Glycol 1500

Abstract In this work, we studied the interphase distribution of a number of polybasic carboxylic acids, in particular, malic, succinic, glutaric, citric, ascorbic and tartaric in two-phase aqueous systems polyethylene glycol-1500 (PEG-1500) - sodium sulfate - water and PEG-1500 - ammonium sulfate - water. Using the spectrophotometric method of analysis, quantitative characteristics (interfacial distribution and degree of recovery) of the extraction of carboxylic acids were determined. In conclusion, it was found that the considered two-phase aqueous systems are promising in the process of extraction of polybasic carboxylic acids. In particular, the system polyethylene glycol 1500 - sodium sulfate - water has effective extraction properties for succinic malic and ascorbic acids. On the other hand, depending on the composition of the extraction systems, the quantitative characteristics for citric, glutaric and tartaric acids practically do not differ.

scholarly journals Effect of sodium chloride on solute–solvent interactions in aqueous polyethylene glycol–sodium sulfate two-phase systems

2015 ◽  
Vol 1425 ◽  
pp. 51-61 ◽  
Author(s):  
Nuno R. da Silva ◽  
Luisa A. Ferreira ◽  
Pedro P. Madeira ◽  
José A. Teixeira ◽  
Vladimir N. Uversky ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Chloride ◽  
Sodium Sulfate ◽  
Two Phase ◽  
Solvent Interactions ◽  
Phase Systems
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