On-chip protein isoelectric focusing using a photoimmobilized pH gradient†

2014 ◽  
Vol 37 (21) ◽  
pp. 3174-3180 ◽  
Author(s):  
Lin Xia ◽  
FengMing Lin ◽  
Xin Wu ◽  
Chuanli Liu ◽  
Jianshe Wang ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Ph Gradient ◽  
On Chip

Multistage Isoelectric Focusing: A Novel On-Chip Bio-Separation Technique

2005 ◽  
Author(s):  
Prashanta Dutta ◽  
Keisuke Horiuchi ◽  
Huanchun Cui ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Fluorescent Protein ◽  
Ph Gradient ◽  
Resolving Power ◽  
Protein Species ◽  
Second Stage ◽  
On Chip ◽  
Bonding Technique

This experimental study reports a method to increase the resolving power of isoelectric focusing (IEF) on a polymeric microfluidic chip. Microfluidic chip is formed on poly-di-methyl siloxane (PDMS) using soft lithography and multilayer bonding technique. In this novel bioseparation technique, IEF is staged by first focusing protein species in a straight channel using broad-range ampholytes and then refocusing segments of that first channel into secondary channels that branch out from the first one. Experiments demonstrated that three fluorescent protein species within a segment of pH gradient in the first stage were refocused in the second stage with much higher resolution in a shallower pH gradient. A serially performed two-stage IEF was completed in less than 25 minutes under particularly small electric field strength up to 100 V/cm.

scholarly journals On-chip protein isoelectric focusing using a photo-immobilized pH gradient

2014 ◽  
Vol 37 (21) ◽  
pp. NA-NA
Author(s):  
Lin Xia ◽  
FengMing Lin ◽  
Xin Wu ◽  
Chuanli Liu ◽  
Jianshe Wang ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Ph Gradient ◽  
On Chip

Instability and non-linearity of the pH gradient formed in isoelectric focusing

1979 ◽  
Vol 174 (1) ◽  
pp. 1-11 ◽  
Author(s):  
A. Murel ◽  
I. Kirjanen ◽  
O. Kirret
Keyword(s):  
Isoelectric Focusing ◽  
Ph Gradient

Focusing of Proteins in a Horseshoe Microchannel

2008 ◽  
Author(s):  
Jaesool Shim ◽  
Prashanta Dutta ◽  
Cornelius F. Ivory
Keyword(s):  
Capillary Electrophoresis ◽  
Electric Field ◽  
Isoelectric Focusing ◽  
Ph Gradient ◽  
Complex Geometries ◽  
Electrokinetic Phenomena ◽  
Double Peaks ◽  
Zone Electrophoresis ◽  
Carrier Ampholytes ◽  
Ph Range

Ampholyte based isoelectric focusing (IEF) simulation was conducted to study dispersion of proteins in a horseshoe microchannel. Four model proteins (pls = 6.49, 7.1, 7.93 and 8.6) are focused in a 1 cm long horseshoe channel under an electric field of 300 V/cm. The pH gradient is formed in the presence of 25 biprotic carrier ampholytes (ΔpK = 3.0) within a pH range of 6 to 9. The proteins are focused at 380 sec in a nominal electric field of 300 V/cm. Our numerical results show that the band dispersions of a protein are large during the marching stage, but the dispersions are significantly reduced when the double peaks start to merge. This rearrangement of spreading band is very unique compared to linear electrokinetic phenomena (capillary electrophoresis, zone electrophoresis or electroosmosis) and is independent of channel position and channel shape. Hence, one can perform IEF in complex geometries without incorporating hyperturns.

Effects of ampholyte concentration on protein behavior in on-chip isoelectric focusing

2008 ◽  
Vol 29 (5) ◽  
pp. 1026-1035 ◽  
Author(s):  
Jaesool Shim ◽  
Prashanta Dutta ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
On Chip

Differential Radioactive Proteomic Analysis of Microdissected Renal Cell Carcinoma Tissue by 54 cm Isoelectric Focusing in Serial Immobilized pH Gradient Gels

2005 ◽  
Vol 4 (6) ◽  
pp. 2117-2125 ◽  
Author(s):  
Slobodan Poznanović ◽  
Wojciech Wozny ◽  
Gerhard P. Schwall ◽  
Chaturvedula Sastri ◽  
Christian Hunzinger ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Isoelectric Focusing ◽  
Proteomic Analysis ◽  
Renal Cell ◽  
Ph Gradient ◽  
Carcinoma Tissue ◽  
Renal Cell Carcinoma Tissue

Study on stability mechanism of immobilized pH gradient in isoelectric focusing via the Svensson–Tiselius differential equation and moving reaction boundary

Talanta ◽  
2013 ◽  
Vol 111 ◽  
pp. 20-27 ◽  
Author(s):  
Chen-Gang Guo ◽  
Si Li ◽  
Hou-Yu Wang ◽  
Dong Zhang ◽  
Guo-Qing Li ◽  
...  
Keyword(s):  
Differential Equation ◽  
Isoelectric Focusing ◽  
Ph Gradient
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