Elucidation of the retention mechanism on a reverse-phase cyano column by modeling

2014 ◽  
Vol 37 (15) ◽  
pp. 1919-1929 ◽  
Author(s):  
Maria G. Kouskoura ◽  
Dimitra Hadjipavlou-Litina ◽  
Catherine K. Markopoulou
Keyword(s):  
Retention Mechanism ◽  
Reverse Phase ◽  
Cyano Column

Nachweis von Aromatase-Expression im eutopen Endometrium mittels reverse-phase Protein Arraying bei Patientinnen mit Endometriose

2008 ◽  
Vol 68 (S 01) ◽  
Author(s):  
MM Wölfler ◽  
C Siehs ◽  
K Schwamborn ◽  
D Otten ◽  
R Knüchel-Clarke ◽  
...  
Keyword(s):  
Reverse Phase ◽  
Aromatase Expression ◽  
Phase Protein

Preparation and Characterization of NH2-Terminal Fibrinogen Bβ Fragments from N-DSK of Human Fibrinogen

1984 ◽  
Vol 51 (01) ◽  
pp. 016-021 ◽  
Author(s):  
S Birken ◽  
G Agosto ◽  
B Lahiri ◽  
R Canfield
Keyword(s):  
High Performance ◽  
Gel Filtration ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Reverse Phase ◽  
Human Fibrinogen ◽  
Human Volunteers ◽  
Cnbr Cleavage ◽  
Two Peaks

SummaryIn order to investigate the early release of NH2-terminal plasmic fragments from the Bβ chain of fibrinogen, substantial quantities of Bβ 1-42 and Bβ 1-21 are required as immunogens, as radioimmunoassay standards and for infusion into human volunteers to determine the half-lives of these peptides. Towards this end methods that employ selective proteolytic cleavage of these fragments from fibrinogen have been developed. Both the N-DSK fragment, produced by CNBr cleavage of fibrinogen, and Bβ 1-118 were employed as substrates for plasmin with the finding of higher yields from N-DSK. Bβ 1-42 and Bβ 1-21 were purified by gel filtration and ion-exchange chromatography on SP-Sephadex using volatile buffers. When the purified preparation of Bβ 1-42 was chromatographed on reverse-phase high performance liquid chromatography, two peaks of identical amino acid composition were separated, presumably due either to pyroglutamate or to amide differences.

Modelling Of Transport And Capture Of Particles In A Porous Medium

2019 ◽  
pp. 56-60
Keyword(s):  
Porous Medium ◽  
Asymptotic Solution ◽  
Retention Mechanism ◽  
Inverse Filtering ◽  
Underground Structures ◽  
Loose Soil ◽  
Monodisperse Suspension ◽  
Homogeneous Porous Medium ◽  
Model Equations ◽  
Pass Through

The study of the transport and capture of particles moving in a fluid flow in a porous medium is an important problem of underground hydromechanics, which occurs when strengthening loose soil and creating watertight partitions for building tunnels and underground structures. A one-dimensional mathematical model of long-term deep filtration of a monodisperse suspension in a homogeneous porous medium with a dimensional particle retention mechanism is considered. It is assumed that the particles freely pass through large pores and get stuck at the inlet of small pores whose diameter is smaller than the particle size. The model takes into account the change in the permeability of the porous medium and the permissible flow through the pores with increasing concentration of retained particles. A new spatial variable obtained by a special coordinate transformation in model equations is small at any time at each point of the porous medium. A global asymptotic solution of the model equations is constructed by the method of series expansion in a small parameter. The asymptotics found is everywhere close to a numerical solution. Global asymptotic solution can be used to solve the inverse filtering problem and when planning laboratory experiments.

Chemometric study of the retention mechanism on butyl column: effect and relation of pH and pKa

2015 ◽  
Vol 33 (12) ◽  
pp. 1274
Author(s):  
Maria G KOUSKOURA ◽  
Constantina V MITANI ◽  
Catherine K MARKOPOULOU
Keyword(s):  
Retention Mechanism
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