Rapid determination of globin chains in red blood cells by capillary electrophoresis using INSTCoated fused-silica capillary

2014 ◽  
Vol 37 (8) ◽  
pp. 1026-1032 ◽  
Author(s):  
Petr Tůma
2013 ◽  
Vol 19 (No. 4) ◽  
pp. 132-138 ◽  
Author(s):  
J. Moravcová ◽  
T. Kleinová

The separation of six isoflavones (biochanin A, isoformononetin, formononetin, prunetin, daidzein and genistein) and coumestrol on an uncoated fused-silica capillary electrophoresis column was optimised using alkaline borate buffer as electrolyte and DAD detection. A baseline separation of all analytes except a pair, formononetin-biochanin A was achieved at pH 10.5 in 25 min. Detection limits were low (0.1 µg/ml) and the linearity of the detector response was established in the concentration range 0.4–60 µg/ml (180 µg/ml for coumestrol). Coumestrol was synthesized and the carbon signals in 13C-NMR spectrum of both coumestrol and di-O-acetylcoumestrol were assigned for the first time using two-dimensional HMQC technique.


2013 ◽  
Vol 850-851 ◽  
pp. 1152-1155
Author(s):  
Hai Xing Liu ◽  
Qing Liu ◽  
Xiao Yang Xu ◽  
Guo Dong Feng ◽  
Lin Tong Wang ◽  
...  

High performance capillary electrophoresis (HPCE) was used in this text to determine the content of ferulic acid in “SHI-QUAN-DA-BU” Pills. Electrophoretic separation conditions: Uncoated fused silica capillary column(75um×49/58cm), injection height 7.5cm, 30mmol•L-1borax solution, separation voltage 20kV, injection time 15s, detection wavelength 315 nm, experimental temperature 20°C. Linearity was kept in the concentration ranging from 1~31.25 mg·L-1of ferulic acid with correlation coefficient of 0.970. The average recovery was 97.56% and RSD value was 6.4%. The content of ferulic acid was 0.0855 mg/g (n=6).


1983 ◽  
Vol 256 ◽  
pp. 303-312 ◽  
Author(s):  
Kazuhiro Kuwata ◽  
Kazuhiko Yamada ◽  
Hiroyasu Yamasaki ◽  
Yoshiaki Yamazaki ◽  
Yoshiyuki Kiso

1993 ◽  
Vol 37 (3) ◽  
pp. 175-181
Author(s):  
Susumu Kurioka ◽  
Keiko Tomaru ◽  
Yasuyo Sato ◽  
Yasuyuki Kurosu

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