Determination of rimantadine in human urine by HPLC using a monolithic stationary phase and on-line post-column derivatization

2013 ◽  
Vol 36 (11) ◽  
pp. 1720-1725 ◽  
Author(s):  
Constantinos K. Zacharis ◽  
Paraskevas D. Tzanavaras ◽  
Athanasios G. Vlessidis
Keyword(s):  
Stationary Phase ◽  
Human Urine ◽  
Monolithic Stationary Phase ◽  
On Line

scholarly journals Fluorimetric Method for the Determination of Histidine in Random Human Urine Based on Zone Fluidics

Molecules ◽  
2020 ◽  
Vol 25 (7) ◽  
pp. 1665
Author(s):  
Antonios Alevridis ◽  
Apostolia Tsiasioti ◽  
Constantinos K. Zacharis ◽  
Paraskevas D. Tzanavaras
Keyword(s):  
Matrix Effect ◽  
Human Urine ◽  
Basic Medium ◽  
Medium Ph ◽  
Line Flow ◽  
On Line ◽  
Adult Volunteers ◽  
Fluorescent Derivative ◽  
Better Than

In the present study, the determination of histidine (HIS) by an on-line flow method based on the concept of zone fluidics is reported. HIS reacts fast with o-phthalaldehyde at a mildly basic medium (pH 7.5) and in the absence of additional nucleophilic compounds to yield a highly fluorescent derivative (λex/λem = 360/440 nm). The flow procedure was optimized and validated, paying special attention to its selectivity and sensitivity. The LOD was 31 nmol·L−1, while the within-day and day-to-day precisions were better than 1.0% and 5.0%, respectively (n = 6). Random urine samples from adult volunteers (n = 7) were successfully analyzed without matrix effect (<1%). Endogenous HIS content ranged between 116 and 1527 μmol·L−1 with percentage recoveries in the range of 87.6%–95.4%.

Direct determination of celiprolol in human urine using on-line coupled ITP-CZE method with fiber-based DAD

2008 ◽  
Vol 29 (22) ◽  
pp. 4561-4567 ◽  
Author(s):  
Peter Mikuš ◽  
Katarína Maráková ◽  
Jozef Marák ◽  
Alexandra Planková ◽  
Iva Valášková ◽  
...  
Keyword(s):  
Human Urine ◽  
Direct Determination ◽  
On Line
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