Application of a micromembrane chromatography module to the examination of protein adsorption equilibrium

2012 ◽  
Vol 35 (22) ◽  
pp. 3177-3183 ◽  
Author(s):  
Natália Káňavová ◽  
Anna Kosior ◽  
Monika Antošová ◽  
René Faber ◽  
Milan Polakovič
Keyword(s):  
Protein Adsorption ◽  
Adsorption Equilibrium

scholarly journals Mesocellular Silica Foams (MCFs) with Tunable Pore Size as a Support for Lysozyme Immobilization: Adsorption Equilibrium and Kinetics, Biocomposite Properties

2020 ◽  
Vol 21 (15) ◽  
pp. 5479
Author(s):  
Agnieszka Chrzanowska ◽  
Anna Derylo-Marczewska ◽  
Malgorzata Wasilewska
Keyword(s):  
Pore Size ◽  
Protein Adsorption ◽  
Pore Diameter ◽  
Adsorption Equilibrium ◽  
Thermal Characteristics ◽  
Equilibrium And Kinetics ◽  
Potential Applications ◽  
Adsorption Equilibrium And Kinetics ◽  
Loading Amount ◽  
Mesocellular Silica Foams

The effect of the porous structure of mesocellular silica foams (MCFs) on the lysozyme (LYS) adsorption capacity, as well as the rate, was studied to design the effective sorbent for potential applications as the carriers of biomolecules. The structural (N2 adsorption/desorption isotherms), textural (SEM, TEM), acid-base (potentiometric titration), adsorption properties, and thermal characteristics of the obtained lysozyme/silica composites were studied. The protein adsorption equilibrium and kinetics showed significant dependence on silica pore size. For instance, LYS adsorption uptake on MCF-6.4 support (pore diameter 6.4 nm) was about 0.29 g/g. The equilibrium loading amount of LYS on MCF-14.5 material (pore size 14.5 nm) increased to 0.55 g/g. However, when the pore diameter was larger than 14.5 nm, the LYS adsorption value systematically decreased with increasing pore size (e.g., for MCF-30.1 was only 0.27 g/g). The electrostatic attractive interactions between the positively charged lysozyme (at pH = 7.4) and the negatively charged silica played a significant role in the immobilization process. The differences in protein adsorption and surface morphology for the biocomposites of various pore sizes were found. The thermal behavior of the studied bio/systems was conducted by TG/DSC/FTIR/MS coupled method. It was found that the thermal degradation of lysozyme/silica composites was a double-stage process in the temperature range 165–420–830 °C.

True and Apparent Temperature Dependence of Protein Adsorption Equilibrium in Reversed-Phase HPLC

2002 ◽  
Vol 18 (6) ◽  
pp. 1306-1317 ◽  
Author(s):  
P. Szabelski ◽  
A. Cavazzini ◽  
K. Kaczmarski ◽  
J. Van Horn ◽  
G. Guiochon
Keyword(s):  
Temperature Dependence ◽  
Protein Adsorption ◽  
Adsorption Equilibrium ◽  
Reversed Phase ◽  
Apparent Temperature ◽  
Reversed Phase Hplc

STUDY OF ADSORPTION EQUILIBRIUM OF SOME WET AIRCOMPOSITE MATERIAL SYSTEMS

2009 ◽  
Vol 8 (2) ◽  
pp. 253-257 ◽  
Author(s):  
Stelian Petrescu ◽  
Ioan Mamaliga ◽  
Constantin Baciu
Keyword(s):  
Adsorption Equilibrium ◽  
Material Systems

Kinetic and thermodynamic study of the adsorption of calcium onto sugar beet pulp

2016 ◽  
pp. 565-570
Author(s):  
Huang Qin ◽  
Zhu Si-ming ◽  
Zeng Di ◽  
Yu Shu-juan
Keyword(s):  
Sugar Beet ◽  
Adsorption Equilibrium ◽  
Ph Value ◽  
Hard Water ◽  
Sugar Beet Pulp ◽  
Order Kinetic ◽  
Factors Affecting ◽  
Isotherm Equation ◽  
Equilibrium Data ◽  
Beet Pulp

Sugar beet pulp (SBP) was used as low value adsorbent for the removal of calcium from hard water. Batch experiments were conducted to determine the factors affecting adsorption of the process such as pH value and Ca concentration. The adsorption equilibrium of Ca2+ by the SBP is reached after 100min and a pseudo second-order kinetic model can describe the adsorption process. The initial concentrations of Ca varied from 927 to 1127mgCa2+/L. A dose of 30g/L sugar beet pulp was sufficient for the optimum removal of calcium. The overall uptake of Ca ions by sugar beet pulp has its maximum at pH=8. The adsorption equilibrium data fitted well with the Langmuir adsorption isotherm equation.

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