Simultaneous determination of six toxic alkaloids in human plasma and urine using capillary zone electrophoresis coupled to time-of-flight mass spectrometry

2012 ◽  
Vol 35 (20) ◽  
pp. 2773-2780 ◽  
Author(s):  
Zhuhong Yu ◽  
Zhongping Wu ◽  
Feijun Gong ◽  
Rong Wong ◽  
Chen Liang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Time Of Flight ◽  
Flight Mass Spectrometry ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Toxic Alkaloids

scholarly journals Simultaneous Determination of Sitagliptin and Metformin in Pharmaceutical Preparations by Capillary Zone Electrophoresis and its Application to Human Plasma Analysis

2012 ◽  
Vol 7 ◽  
pp. ACI.S9940 ◽  
Author(s):  
Mohamed Salim ◽  
Nahed El-Enany ◽  
Fathallah Belal ◽  
Mohamed Walash ◽  
Gabor Patonay
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Pharmaceutical Preparations ◽  
Effective Length ◽  
Relative Standard ◽  
Significant Difference ◽  
Zone Electrophoresis ◽  
Capillary Zone

A novel, quick, reliable and simple capillary zone electrophoresis CZE method was developed and validated for the simultaneous determination of sitagliptin (SG) and metformin (MF) in pharmaceutical preparations. Separation was carried out in fused silica capillary (50.0 cm total length and 43.0 cm effective length, 49 μm i.d.) by applying a potential of 15 KV (positive polarity) and a running buffer containing 60 mM phosphate buffer at pH 4.0 with UV detection at 203 nm. The samples were injected hydrodynamically for 3 s at 0.5 psi and the temperature of the capillary cartridge was kept at 25 °C. Phenformin was used as internal standard (IS). The method was suitably validated with respect to specificity, linearity, limit of detection and quantitation, accuracy, precision, and robustness. The method showed good linearity in the ranges of 10-100 μg/mL and 50-500 μg/mL with limits of detection of 0.49, 2.11 μm/mL and limits of quantification of 1.48, 6.39 μg/mL for SG and MF, respectively. The proposed method was successfully applied for the analysis of the studied drugs in their synthetic mixtures and co-formulated tablets without interfering peaks due to the excipients present in the pharmaceutical tablets. The method was further extended to the in-vitro determination of the two drugs in spiked human plasma. The estimated amounts of SG/MF were almost identical with the certified values, and their percentage relative standard deviation values (% R.S.D.) were found to be ≤1.50% (n = 3). The results were compared to a reference method reported in the literature and no significant difference was found statistically.

Capillary zone electrophoresis (CZE) coupled to time-of-flight mass spectrometry (TOF-MS) applied to the analysis of illicit and controlled drugs in blood

2008 ◽  
Vol 29 (19) ◽  
pp. 4078-4087 ◽  
Author(s):  
Rossella Gottardo ◽  
Aldo Polettini ◽  
Daniela Sorio ◽  
Jennifer Paola Pascali ◽  
Federica Bortolotti ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Capillary Zone Electrophoresis ◽  
Time Of Flight ◽  
Flight Mass Spectrometry ◽  
Controlled Drugs ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Drugs In Blood ◽  
Tof Ms

Simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by capillary zone electrophoresis

2004 ◽  
Vol 18 (1) ◽  
pp. 51-54 ◽  
Author(s):  
Daisuke Teshima ◽  
Kaori Otsubo ◽  
Kazutaka Makino ◽  
Yoshinori Itoh ◽  
Ryozo Oishi
Keyword(s):  
Simultaneous Determination ◽  
Human Plasma ◽  
Capillary Zone Electrophoresis ◽  
Zone Electrophoresis ◽  
Capillary Zone
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