Tracking antiangiogenic components from Glycyrrhiza uralensis Fisch. based on zebrafish assays using high-speed countercurrent chromatography

2012 ◽  
Vol 35 (9) ◽  
pp. 1167-1172 ◽  
Author(s):  
Liwen Han ◽  
Yanqiang Yuan ◽  
Liang Zhao ◽  
Qiuxia He ◽  
Yubo Li ◽  
...  
Keyword(s):  
High Speed ◽  
Glycyrrhiza Uralensis ◽  
Countercurrent Chromatography ◽  
Glycyrrhiza Uralensis Fisch

scholarly journals Preparative Separation and Purification of Liquiritin and Glycyrrhizic Acid from Glycyrrhiza uralensis Fisch by High-Speed Countercurrent Chromatography

2020 ◽  
Vol 58 (9) ◽  
pp. 823-830
Author(s):  
Hao Wang ◽  
Hu Shan ◽  
Haitao Lü
Keyword(s):  
Crude Extract ◽  
High Speed ◽  
Glycyrrhizic Acid ◽  
Retention Rate ◽  
Glycyrrhiza Uralensis ◽  
Ionization Mass ◽  
Countercurrent Chromatography ◽  
Separation And Purification ◽  
Isolation And Purification ◽  
Glycyrrhiza Uralensis Fisch

Abstract The technique of high-speed countercurrent chromatography (HSCCC) was applied to the preparative isolation and purification of liquiritin and glycyrrhizic acid from a crude extract of Glycyrrhiza uralensis Fisch for the first time. Using single factor and orthogonal design experiments, the best extraction conditions were 70% ethanol, 1:25 ratio of solid-to-liquid (w/v) and extracted 1.5 h at 80°C. The contents of liquiritin and glycyrrhizic acid in the crude extract were 1.3 and 5.3%, respectively. Using the two-phase solvent system of ethyl acetate–methanol–water (5:2:5, v/v), 6.0 mg liquiritin (the purity was 96.7%, and the recovery was 89.3%), and 20.5 mg glycyrrhizic acid (the purity was 98.9%, and the recovery was 77.1%) were obtained from 500 mg crude extraction by HSCCC, respectively. The retention rate of stationary phase was 51.0%. Their structures were identified by high-performance liquid chromatography, melting points, ultraviolet radiation, Fourier transform infrared (FTIR), Electrospray ionization mass spectrometry (ESI-MS), 1H Nuclear Magnetic Resonance (NMR) and 13C NMR spectra. The scavenging abilities of glycyrrhizic acid to 1,1-diphenyl-2-picrylhydrazyl and hydroxyl free radicals were stronger than those of liquiritin.

Isolation of glucosinolates from broccoli seeds by use of high-speed countercurrent chromatography

2008 ◽  
Vol 20 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Q. Du ◽  
L. Zhang ◽  
H. Cui ◽  
K. Wang ◽  
L. Xiu ◽  
...  
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography

scholarly journals The complete mitochondrial genome of Glycyrrhiza uralensis Fisch. (Fabales, Leguminosae)

2021 ◽  
Vol 6 (2) ◽  
pp. 475-477
Author(s):  
Yan-Yun Yang ◽  
Sheng-Nan Li ◽  
Liang Xu ◽  
Yan-Ping Xing ◽  
Rong Zhao ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Complete Mitochondrial Genome ◽  
Glycyrrhiza Uralensis ◽  
Glycyrrhiza Uralensis Fisch

Separation of anti-ulcer flavonoids from Artemisia extracts by high-speed countercurrent chromatography

2011 ◽  
Vol 129 (2) ◽  
pp. 679-683 ◽  
Author(s):  
Kee Dong Yoon ◽  
Young-Won Chin ◽  
Min Hye Yang ◽  
Jinwoong Kim
Keyword(s):  
High Speed ◽  
Countercurrent Chromatography
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