Preparation of monomeric and polymeric β-cyclodextrin functionalized monoliths for rapid isolation and purification of puerarin from Radix puerariae

2011 ◽  
Vol 34 (16-17) ◽  
pp. 2131-2137 ◽  
Author(s):  
Yongqin Lv ◽  
Timothy C. Hughes ◽  
Xiaojuan Hao ◽  
Danping Mei ◽  
Tianwei Tan
Keyword(s):  
Isolation And Purification ◽  
Rapid Isolation ◽  
Radix Puerariae

Rapid isolation and purification of antibody to factor VIII by protein A

1979 ◽  
Vol 14 (6) ◽  
pp. 925-930 ◽  
Author(s):  
Helen Lee ◽  
Derek Tucker ◽  
J.P. Allain
Keyword(s):  
Factor Viii ◽  
Protein A ◽  
Isolation And Purification ◽  
Rapid Isolation

scholarly journals A standardized method to purify cardiomyocytes from individual mouse hearts of any age

2021 ◽  
Author(s):  
Amy M Nicks ◽  
Sara R Holman ◽  
Andrea Y Chan ◽  
Michael Tsang ◽  
Paul E Young ◽  
...  
Keyword(s):  
Heart Development ◽  
Enzymatic Digestion ◽  
High Yield ◽  
Coronary Perfusion ◽  
Isolation And Purification ◽  
Immunomagnetic Bead ◽  
Rapid Isolation ◽  
Neonatal Cardiomyocytes ◽  
Standardized Method

Rationale: Primary cardiomyocytes are invaluable for understanding postnatal heart development and elucidating disease mechanisms in genetic and pharmacological models, however, a method to obtain freshly purified cardiomyocytes at any postnatal age, without using different age-dependent isolation procedures and cell culture, is lacking. Objective: To develop a standardized method that allows rapid isolation and purification of cardiomyocytes in high yield and viability from individual neonatal, infant, and adult mice. Methods and Results: Hearts of C57BL/6J mice were cannulated using a novel in situ aortic cannulation procedure optimized to allow cannulation of even the very small vessel of neonates (postnatal day 0-2, P0-2). Hearts were then subjected to Langendorff retrograde perfusion and enzymatic digestion. Cardiomyocytes were isolated after subsequent tissue disaggregation and filtration, in high yield (1.56-2.2x106 cardiomyocytes/heart) and viability (~70-100%). The larger size of infant (P10 and P13) and adult (P70), but not neonatal, cardiomyocytes relative to non-myocytes, allowed enrichment by differential centrifugation. Cardiomyocytes from all ages were further purified by immunomagnetic bead-based depletion of non-myocytes. Together, these procedures resulted in the isolation of highly purified cardiomyocytes (~94%) within 1 hour, enabling experiments using individual replicates. For example, RNA-sequencing of cardiomyocytes purified from one P2 male and female heart per litter (n=4 litters) showed distinct clustering by litters and sex differences for nine differentially expressed genes (FDR<0.005). In situ fixation via coronary perfusion, performed immediately after tissue digestion, preserved the cytoarchitecture of isolated cardiomyocytes (yielding ~94% rod-shaped cardiomyocytes at all ages), allowing capture of spindle-shaped neonatal cells undergoing mitosis, as well as enabling accurate quantitation of cardiomyocyte area and nucleation state. Conclusion: The procedures developed here provide a universal protocol for the rapid isolation and purification of high-quality cardiomyocytes from hearts of any postnatal age, even those of neonates, thereby enabling direct comparisons between individual hearts.

scholarly journals Rapid Isolation And Purification Of Mitochondria For Transplantation By Tissue Dissociation And Differential Filtration

10.3791/51682 ◽  
2014 ◽  
Author(s):  
Janine M. Preble ◽  
Christina A. Pacak ◽  
Hiroshi Kondo ◽  
Allison A. MacKay ◽  
Douglas B. Cowan ◽  
...  
Keyword(s):  
Isolation And Purification ◽  
Rapid Isolation ◽  
Tissue Dissociation ◽  
Differential Filtration

Rapid isolation and purification of functional platelet mitochondria using a discontinuous Percoll gradient

Platelets ◽  
2019 ◽  
Vol 31 (2) ◽  
pp. 258-264 ◽  
Author(s):  
Jacob L. Léger ◽  
Jean-Luc Jougleux ◽  
Fanta Savadogo ◽  
Nicolas Pichaud ◽  
Luc H. Boudreau
Keyword(s):  
Percoll Gradient ◽  
Isolation And Purification ◽  
Rapid Isolation ◽  
Discontinuous Percoll Gradient
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