Capillary columns for reversed-phase CEC prepared via surface functionalization of polymer monolith with aromatic selectors

2011 ◽  
pp. n/a-n/a ◽  
Author(s):  
Mohamed Guerrouache ◽  
Marie Claude Millot ◽  
Benjamin Carbonnier
Keyword(s):  
Surface Functionalization ◽  
Capillary Columns ◽  
Reversed Phase ◽  
Polymer Monolith

A polymer monolith for hydrophilic and dynamically surfactant-modified reversed-phase capillary electrochromatography

RSC Advances ◽  
2013 ◽  
Vol 3 (44) ◽  
pp. 21888 ◽  
Author(s):  
Xucong Lin ◽  
Wenchao Jia ◽  
Shuhui Feng ◽  
Jia Lin ◽  
Zenghong Xie
Keyword(s):  
Capillary Electrochromatography ◽  
Reversed Phase ◽  
Polymer Monolith

scholarly journals A new parallel high-pressure packing system enables rapid multiplexed production of capillary columns

2021 ◽  
Author(s):  
Johannes B. Müller-Reif ◽  
Fynn M. Hansen ◽  
Lisa Schweizer ◽  
Peter V. Treit ◽  
Philipp E. Geyer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Particle Size ◽  
High Pressure ◽  
High Performance ◽  
Capillary Columns ◽  
Reversed Phase ◽  
Peptide Separation ◽  
Ultra High Pressure ◽  
Trained Personnel ◽  
Packing System

AbstractReversed-phase high performance liquid chromatography (HPLC) is the most commonly applied peptide separation technique in mass spectrometry (MS)-based proteomics. Particle-packed capillary columns are predominantly used in nano-flow HPLC systems. Despite being the broadly applied standard for many years capillary columns are still expensive and suffer from short lifetimes, particularly in combination with ultra-high-pressure chromatography systems. For this reason, and to achieve maximum performance, many laboratories produce their own in-house packed columns. This typically requires a considerable amount of time and trained personnel. Here, we present a new packing system for capillary columns enabling rapid, multiplexed column production with pressures reaching up to 3000 bar. Requiring only a conventional gas pressure supply and methanol as driving fluid, our system replaces the traditional setup of helium pressured packing bombs. By using 10x multiplexing, we have reduced the production time to just under 2 minutes for several 50 cm columns with 1.9 µm particle size, speeding up the process of column production 40 to 800 times. We compare capillary columns with various inner diameters (ID) and length packed under different pressure conditions with our newly designed, broadly accessible high-pressure packing station.One sentence summaryA newly constructed parallel high-pressure packing system enables the rapid multiplexed production of capillary columns.Abstract Figure

Sign in / Sign up

Export Citation Format

Share Document