Quantitation of antioxidants in water samples using ionic liquid dispersive liquid-liquid microextraction followed by high-performance liquid chromatography-ultraviolet detection

2010 ◽  
Vol 34 (1) ◽  
pp. 77-82 ◽  
Author(s):  
Hamid Reza Sobhi ◽  
Amir Kashtiaray ◽  
Hadi Farahani ◽  
Mohammad Reza Farahani
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Water Samples ◽  
High Performance ◽  
Ultraviolet Detection ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

scholarly journals Ionic liquid-based dispersive liquid-liquid microextraction combined with high performance liquid chromatography-UV detection for simultaneous preconcentration and determination of Ni, Co, Cu and Zn in water samples

2014 ◽  
Vol 79 (1) ◽  
pp. 63-76 ◽  
Author(s):  
Alireza Asghari ◽  
Mehri Ghazaghi ◽  
Maryam Rajabi ◽  
Mahdi Behzad ◽  
Mehrorang Ghaedi
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Water Samples ◽  
High Performance ◽  
Chelating Agent ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Cu And Zn

Ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) coupled with high performance liquid chromatography (HPLC)-UV detection was developed for simultaneous extraction and determination of nickel, cobalt, copper and zinc ions. In the proposed approach, salophen (N,N'-bis(salisyliden)-1,2-phenylenediamine) was used as a chelating agent; the ionic liquid, 1-hexeyl-3-methylimidazolium hexafluorophosphate, and acetone were selected as extracting and dispersive solvents, respectively. After extraction, phase separation was performed by centrifugation and the sedimented phase (ionic liquid) was solubilized in acetonitrile and directly injected into the HPLC for subsequent analysis. Baseline separation of metal ion complexes was achieved on a RP-C18 column using a gradient elution of the mixtures of methanol-acetonitrile-water as the mobile phase at a flow rate of 1.0 mL min-1. The influence of variables such as sample pH, concentration of the chelating agent, amount of ionic liquid (extraction solvent), disperser solvent volume, extraction time, salt effect and centrifugation speed were studied and optimized. Under the optimum conditions, the enrichment factor of 222 was obtained. The detection limits for Ni, Co, Cu and Zn were 0.8, 1.6, 1.9 and 2.8 ?g L?1, respectively. The relative standard deviations (RSDs) were in the range of 3.6-5.0 % for all of the investigated metal ions. The proposed procedure was successfully applied to the determination of the studied metal ions in water samples.

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