A novel pathogen detection system based on high-resolution CE-SSCP using a triblock copolymer matrix

2010 ◽  
Vol 33 (11) ◽  
pp. 1639-1643 ◽  
Author(s):  
Gi Won Shin ◽  
Hee Sung Hwang ◽  
Sang Woo Seo ◽  
Mi-Hwa Oh ◽  
Chang Y. Ryu ◽  
...  
2010 ◽  
Vol 31 (6) ◽  
pp. 1108-1115 ◽  
Author(s):  
Se Jin Kim ◽  
Gi Won Shin ◽  
Seok Jin Choi ◽  
Hee Sung Hwang ◽  
Gyoo Yeol Jung ◽  
...  

2005 ◽  
Author(s):  
J M Dzenitis ◽  
A J Makarewicz ◽  
D R Hadley ◽  
D M Gutierrez ◽  
T R Metz ◽  
...  

2020 ◽  
Vol 26 (S2) ◽  
pp. 1806-1807
Author(s):  
Jaroslav Jiruše ◽  
Petr Sytař ◽  
Jan Páral ◽  
Tomáš Hrnčíř

2018 ◽  
Vol 101 (5) ◽  
pp. 1584-1592
Author(s):  
Kakolie Banerjee ◽  
Brittney Pierson ◽  
Chuxuan Hu ◽  
Elijah Carrier ◽  
Lauren Malsick ◽  
...  

Abstract Background: Listeria spp. are an important foodborne human pathogen because of their ability to cause disease and high mortality in individuals, particularly pregnant women, neonates, the elderly, immunocompromised individuals, and children. The Sample6 DETECTTM HT/L Kit is a semi-automated qualitative pathogen detection system designed to detect Listeria spp. (L. monocytogenes, L. innocua, L. ivanovii, L. seeligeri, L. welshimeri, and L. marthii) in environmental samples using the Sample6 BioIlluminationTM technology. Objective: The study was done to evaluate the Sample6 DETECT HT/L Kit. The assay was evaluated for inclusivity, exclusivity, robustness, product consistency, and stability, and a matrix study of one environmental surface. Methods: The performance of the Sample6 DETECT HT/L was compared with U.S. Food and Drug Administration reference culture method for Listeria using an unpaired study design. Results: The Sample6 DETECT HT/L assay correctly identified all 50 inclusivity isolates and correctly excluded all 30 nontarget strains evaluated. The assay was not affected by minor variations in incubation temperature and time, or sample volume. Results across three production lots spanning the shelf life of the assay were consistent. In the matrix study, the Sample6 DETECT HT/L for Listeria correctly identified each test portion for the presence or absence of Listeria, and there were no statistically significant differences between candidate and reference method results. Conclusions: The data collected in this study demonstrate that the Sample6 DETECT HT/L assay is a reliable method for the detection of Listeria spp. on stainless-steel environmental surfaces after 22 h of enrichment.


2020 ◽  
Vol 152 ◽  
pp. 112007 ◽  
Author(s):  
Won-Il Lee ◽  
Younghyeon Park ◽  
Sajal Shrivastava ◽  
Taekeon Jung ◽  
Montri Meeseepong ◽  
...  

2001 ◽  
Vol 7 (S2) ◽  
pp. 234-235
Author(s):  
RE. Batson ◽  
H.W. Mook ◽  
P. Kruit ◽  
O.L. Krivanek ◽  
N. Dellby

The IBM high resolution STEM project has now reached the point where integration of new subsystems into the instrument is the main activity. During the past three years, we have demonstrated a 50meV EELS resolution using a high brightness electron monochromator incorporated into the electron gun [1, 2, 3], and better than 0.14mn spatial resolution using spherical aberration correction at 120KeV. [4] Recent effort has been to identify and remove many sources of instabilities in the original instrument; to rebuild several existing subsidiary pieces of equipment such as the ADF detector, the EELS spectrometer electron optical coupling, and the EELS CCD detection system; and to set up software and hardware control of each system to allow routine operation.The new STEM column is summarized in Fig. 1. Briefly, the original VG gun flange has been lowered about 4cm, using a spacer, to allow insertion of the Fringe Field monochromator.


Author(s):  
F. P. Milanovich ◽  
J. Dzenitis ◽  
B. J. Hindson ◽  
A. J. Makarewicz ◽  
M. T. McBride ◽  
...  

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