scholarly journals Quantitative analysis and chromatographic fingerprinting for the quality evaluation of Forsythia suspensa extract by HPLC coupled with photodiode array detector

2009 ◽  
Vol 32 (23-24) ◽  
pp. 4113-4125 ◽  
Author(s):  
Yonggang Xia ◽  
Bingyou Yang ◽  
Qiuhong Wang ◽  
Jun Liang ◽  
Youhe Wei ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Quality Evaluation ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Forsythia Suspensa ◽  
Photodiode Array ◽  
Chromatographic Fingerprinting

Quantitative analysis of fourteen synthetic dyes in jelly and gummy candy by ultra performance liquid chromatography

2014 ◽  
Vol 6 (15) ◽  
pp. 5872-5878 ◽  
Author(s):  
Yi Yang ◽  
Jing Zhang ◽  
Bing Shao
Keyword(s):  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Simultaneous Determination ◽  
Analytical Method ◽  
Ultra Performance Liquid Chromatography ◽  
Array Detector ◽  
Synthetic Dyes ◽  
Photodiode Array Detector ◽  
Photodiode Array

An analytical method was developed for the simultaneous determination of 14 dyes in jelly and gummy candy using ultra performance liquid chromatography with a photodiode array detector.

scholarly journals Development and validation of an HPLC method for the determination of fluorouracil in polymeric nanoparticles

2013 ◽  
Vol 49 (1) ◽  
pp. 117-126 ◽  
Author(s):  
Ana Cristina de Mattos ◽  
Najeh Maissar Khalil ◽  
Rubiana Mara Mainardes
Keyword(s):  
Quantitative Analysis ◽  
Flow Rate ◽  
High Performance ◽  
Polymeric Nanoparticles ◽  
Hplc Method ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Relative Standard ◽  
Photodiode Array ◽  
Development And Validation

The objective of this work was to develop and validate a rapid high performance liquid chromatography (HPLC) method for the quantitative analysis of fluorouracil (5-FU) in polymeric nanoparticles. Chromatographic analyses were performed on an RP C18 column with a mobile phase consisting of acetonitrile and water (10:90, v/v) at a flow rate of 1 mL/min. The 5-FU was detected and quantitated using a photodiode array detector at a wavelength of 265 nm. The method was shown to be specific and linear in the range of 0.1-10 µg/mL (r = 0.9997). The precision (intra- and inter-day) was demonstrated because the maximum relative standard deviation was 3.51%. The method is robust relative to changes in flow rate, column and temperature. The limits of detection and quantitation were 10.86 and 32.78 ng/mL, respectively. The method fulfilled the requirements for reliability and feasibility for application to the quantitative analysis of 5-FU in polymeric nanoparticles.

Determination of Niacin in Infant Formula by Solid-phase Clean-up and HPLC with Photodiode Array Detector

2009 ◽  
Vol 38 (3) ◽  
pp. 359-363
Author(s):  
Jee-Eun Hong ◽  
Mi-Ran Kim ◽  
Sang-Hee Cheon ◽  
Jung-Young Chai ◽  
Eun-Ryong Park ◽  
...  
Keyword(s):  
Infant Formula ◽  
Solid Phase ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array

Development and Validation of an HPLC Method for Determination of Spirotetramat and Spirotetramat cis enol in Various Vegetables and Soil

2013 ◽  
Vol 96 (3) ◽  
pp. 670-675 ◽  
Author(s):  
Balwinder Singh ◽  
Kousik Mandal ◽  
Sanjay K Sahoo ◽  
Urvashi Bhardwaj ◽  
Raminderjit Singh Battu
Keyword(s):  
Analytical Method ◽  
Anhydrous Sodium Sulfate ◽  
Hplc Method ◽  
Array Detector ◽  
Photodiode Array Detector ◽  
Photodiode Array ◽  
Precision And Accuracy ◽  
Hplc Grade Acetonitrile ◽  
Development And Validation

Abstract An easy and simple analytical method was standardized and validated for the estimation of residues of spirotetramat and its metabolite spirotetramat cis enol in various substrates: okra fruits, brinjal leaves and fruits, green chili, red chili, and soil. The samples were extracted with acetonitrile, diluted with brine solution, partitioned into dichloromethane, dried over anhydrous sodium sulfate, and cleaned up by treatment with activated charcoal powder. Final clear extracts were concentrated under vacuum and reconstituted with HPLC grade acetonitrile. Residues were estimated using HPLC with a photodiode array detector and a C18 column, and confirmed by HPTLC. Acetonitrile was used as the mobile phase at 0.4 mL/min. Both spirotetramat and spirotetramat cis enol presented distinct peak at retention times of 8.518 and 7.598 min, respectively. Consistent recoveries ranging from 82 to 97% for spirotetramat and spirotetramat cis enol were observed when samples were spiked at 1.00 to 0.03 mg/kg levels. The LOQ of the method was found to be 0.03 mg/kg. The analytical method was validated in terms of parameters, including selectivity, linearity, precision, and accuracy.

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