Dispersive liquid-liquid microextraction followed by reversed phase HPLC for the determination of decabrominated diphenyl ether in natural water

2008 ◽  
Vol 31 (13) ◽  
pp. 2371-2376 ◽  
Author(s):  
Yanyan Li ◽  
Jia Hu ◽  
Xiujuan Liu ◽  
Lingyan Fu ◽  
Xiaojun Zhang ◽  
...  
Keyword(s):  
Natural Water ◽  
Diphenyl Ether ◽  
Reversed Phase ◽  
Reversed Phase Hplc ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

scholarly journals Determination of Ketosteroids in Human Urine Using Dispersive Liquid-Liquid Microextraction and Ultra High-Performance Liquid Chromatography-High Resolution Mass Spectrometry

2021 ◽  
Vol 76 (11) ◽  
pp. 1305-1311
Author(s):  
E. V. Dmitrieva ◽  
A. Z. Temerdashev ◽  
A. K. Osipova
Keyword(s):  
Mass Spectrometry ◽  
Human Urine ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
High Sensitivity ◽  
Reversed Phase ◽  
Flight Mass Spectrometry ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract A method is proposed for the determination of some ketosteroids in human urine, including enzymatic hydrolysis using β-glucuronidase from E. coli followed by dispersive liquid-liquid microextraction, derivatization of analytes with hydroxylamine, and detection by reversed-phase ultra-HPLC–quadrupole time-of-flight mass spectrometry. Optimization of extraction and derivatization conditions of the studied compounds made it possible to find that the highest recoveries were achieved using an acetone–chloroform mixture as a dispersant and an extractant, and the completeness of the derivatization reaction was achieved by thermostating the sample at 70°C for 90 min. The proposed method has high sensitivity (limits of detection in the range of 0.1–0.25 ng/mL) and a wide linearity range.

Sign in / Sign up

Export Citation Format

Share Document