Performance of R-N(R′)-R′′ functionalised poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) monolithic sorbent for plasmid DNA adsorption

2007 ◽  
Vol 30 (17) ◽  
pp. 2843-2850 ◽  
Author(s):  
Michael K. Danquah ◽  
Jenny Ho ◽  
Gareth M. Forde
2020 ◽  
Vol 20 (5) ◽  
pp. 1143
Author(s):  
Ahmed Ali Alkarimi ◽  
Kevin Welham

Solid-phase extraction technique is considered as a vital tool in a pre-treatment of different samples. Therefore, it was used as a beneficial and alternative extraction method over the liquid–liquid extraction in diverse applications such as food, environmental, and biological analyses. Glycidyl methacrylate-co-stearyl methacrylate-co-ethylene glycol dimethacrylate (GMA-co-SMA-co-EDMA) monolithic sorbent was prepared as strong cationic-reversed phase sorbent for solid-phase extraction of different proteins. The monolithic sorbent was prepared inside two moulds such as glass microchip device and borosilicate. The morphological properties have been investigated using the Brunauer-Emmett-Teller (BET) model analyzer and scanning electron microscope (SEM), and the formation of the monolithic sorbent was examined using the FT-IR. Eight proteins (cytochrome C, insulin, myoglobin, lysozyme, β-lactoglobulin, trypsin, albumin chicken egg white, and apo-transferrin) with different molecular weight and isoelectric point were investigated for pre-concentration using the SCX/RP sorbent. It was found that the highest extraction recovery was obtained with three proteins cytochrome C, lysozyme, and myoglobin compared with the other proteins. These three proteins were chosen for pre-concentration using the glass microchip. Sorbent performance showed significant results in terms of stability and reproducibility.


RSC Advances ◽  
2014 ◽  
Vol 4 (81) ◽  
pp. 42965-42970 ◽  
Author(s):  
Lin Zhou ◽  
Xiaoxiao Li ◽  
Yong Wang ◽  
Mei Hong ◽  
Yongye Liang ◽  
...  

A monodisperse Pt/ceria hybrid with controlled nanoporous structure was fabricated with the aid of poly(glycidyl methacrylate-co-ethylene glycol dimethacrylate) microspheres as a hard template.


2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Luciana C. Costa ◽  
Rafaela C. Monteiro ◽  
Helena M. A. Castro ◽  
Tatiane S. Ribeiro ◽  
Mirian A. Oliveira ◽  
...  

2018 ◽  
Vol 81 (11) ◽  
pp. 1467-1474 ◽  
Author(s):  
Florian Meischl ◽  
Klemens Losso ◽  
Christian G. Kirchler ◽  
Stefan E. Stuppner ◽  
Christian W. Huck ◽  
...  

2014 ◽  
Vol 79 (10) ◽  
pp. 1249-1261 ◽  
Author(s):  
Sanja Marinovic ◽  
Aleksandra Milutinovic-Nikolic ◽  
Aleksandra Nastasovic ◽  
Marija Zunic ◽  
Zorica Vukovic ◽  
...  

Macroporous nanocomposite of poly (glycidyl methacrylate-co-ethylene glycol dimethacrylate) and acid modified bentonite was prepared by radical suspension copolymerization. Nanocomposite was functionalized with diethylene triamine (deta), by ring-opening reaction of the pendant epoxy groups. Functionalization was performed in order to enable phenol derivatives sorption. This new, not sufficiently investigated material, with developed porous structure was denoted CP-SA-deta. In this study, the influence of temperature on 4-nitrophenol (4NP) sorption on CP-SA-deta was investigated. The chemisorption was estimated as dominant process since activation energy of sorption of 4NP of 54.8 kJ mol-1 was obtained. After determining the optimal sorption conditions for 4NP, the sorption of 2-nitrophenol (2NP) and 2-chloro 4-nitrophenol (2Cl4NP) on CP-SA-deta was investigated with respect to pH, initial concentration and contact time. The 2NP sorption was seldom tested, while according to our knowledge, the 2Cl4NP sorption was not investigated. The isotherm data were best fitted with Langmuir model, while the sorption dynamics obeyed the pseudo-second-order kinetic model for all derivatives.


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