Fundamental and practical aspects of ultrahigh pressure liquid chromatography for fast separations

2007 ◽  
Vol 30 (8) ◽  
pp. 1167-1182 ◽  
Author(s):  
Naijun Wu ◽  
Andrew M. Clausen
Keyword(s):  
Liquid Chromatography ◽  
Ultrahigh Pressure ◽  
Fast Separations

Analysis of Squalene and Its Transformation By-Products in Latent Fingermarks by Ultrahigh-Pressure Liquid Chromatography-High Resolution Accurate Mass Orbitrap™ Mass Spectrometry

2019 ◽  
Author(s):  
Buddhika Dorakumbura ◽  
Francesco Busetti ◽  
Simon Lewis
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
High Resolution ◽  
Storage Conditions ◽  
Ultrahigh Pressure ◽  
Accurate Mass ◽  
Rational Approach ◽  
By Products ◽  
Over Time ◽  
High Resolution Accurate Mass

<p>Transformation of squalene and its by-products in fingermarks over time under different storage conditions (light, dark and underwater) was examined through ultrahigh-pressure liquid chromatography high resolution accurate mass Orbitrap™ mass spectrometry. Complications of assessing fingermark compositional variation over time using multiple samples with varying initial compositions were elucidated and a more rational approach was successfully demonstrated. Squalene was detected in all fresh natural fingermarks and the amount ranged between 0.20 to 11.32 μg/5 fingertips. A notable difference in the transformation of squalene was observed with different storage conditions, where a dark aquatic environment accelerated degradation of squalene compared to dark but dry conditions. Squalene monohydroperoxide was extremely short-lived in natural deposits while the amount of squalene epoxide was still increasing relative to the initial amount, after ageing under dark and aquatic conditions for up to 7 days. Some oxidation by-products of cholesterol were also tentatively identified, which exhibited a growth over time against their initial concentration under any of the storage condition tested. These by-products, therefore, show potential as biomarkers for targeted visualisation of aged deposits.</p>

Ultrahigh-pressure liquid chromatography of isoflavones and phenolic acids on different stationary phases

2008 ◽  
Vol 1195 (1-2) ◽  
pp. 52-59 ◽  
Author(s):  
B. Klejdus ◽  
J. Vacek ◽  
L. Lojková ◽  
L. Benešová ◽  
V. Kubáň
Keyword(s):  
Liquid Chromatography ◽  
Phenolic Acids ◽  
Stationary Phases ◽  
Ultrahigh Pressure

scholarly journals Ultrahigh pressure liquid chromatography-atmospheric pressure photoionization-tandem mass spectrometry for the determination of polyphenolic profiles in the characterization and classification of cranberry-based pharmaceutical preparations and natural extracts

2016 ◽  
Vol 8 (22) ◽  
pp. 4363-4378 ◽  
Author(s):  
Lidia Parets ◽  
Élida Alechaga ◽  
Oscar Núñez ◽  
Javier Saurina ◽  
Santiago Hernández-Cassou ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Atmospheric Pressure ◽  
Pharmaceutical Preparations ◽  
Ultrahigh Pressure ◽  
Tandem Mass ◽  
Atmospheric Pressure Photoionization ◽  
Natural Extracts

UHPLC-APPI-MS/MS was used for authentication of fruit-based products and pharmaceutical preparations.

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