Identification of impurities in acarbose by using an integrated liquid chromatography-nuclear magnetic resonance and liquid chromatography-mass spectrometry approach

2005 ◽  
Vol 28 (13) ◽  
pp. 1442-1447 ◽  
Author(s):  
Predrag Novak ◽  
Mario Cindrić ◽  
Predrag Tepeš ◽  
Snježana Dragojević ◽  
Marina Ilijaš ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Nuclear Magnetic

scholarly journals Teknik Isolasi dan Penentuan Struktur Mangiferin: Senyawa Aktif dari Tanaman Mangga (Mangifera indica L.)

2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Luluk Luqyana Zahrah Taqiudina Mahdiyah ◽  
Ahmad Muhtadi ◽  
Aliya Nur Hasanah
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
1H Nmr ◽  
13C Nmr ◽  
Mangifera Indica ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Nuclear Magnetic

Tanaman mangga (Mangifera indica L.) adalah tanaman berbuah musiman yang banyak terdapat di Indonesia. Secara umum, daun tanaman ini mengandung saponin, alkaloid, fenol, tannin, flavonoid, steroid, diterpene dan glikosida. Senyawa metabolit sekunder yang umum ditemukan dalam tanaman ini adalah mangiferin (2-C-β-D-glucopyranosyl-1,3,6,7-tetrahydroxyxanthone), yang termasuk dalam golongan polifenol. Mangiferin memiliki beberapa aktivitas seperti antikanker, antiinflamasi, antidiabetes, dan antihiperlipidemia. Artikel ini membahas teknik isolasi mangiferin dan elusidasi strukturnya yang dilakukan dengan kombinasi spektrofotometri infra merah, Kromatografi Cair-Spektroskopi Massa (Liquid Chromatography-Mass Spectrometry/LC-MS), dan spektroskopi Resonansi Magnetik Inti (Nuclear Magnetic Resonance/NMR) baik berupa 1H-NMR ataupun 13C-NMR. Metode yang digunakan adalah review pustaka dengan pustaka yang digunakan sebanyak 34 artikel yang berasal dari jurnal internasional. Hasil yang didapatkan yaitu isolasi mangiferin dari tanaman mangga dapat dilakukan dengan metode refluks, soxhletasi, partisi tiga fase atau dengan bantuan microwave. Dari hasil tersebut, ditemukan bahwa ekstraksi dengan bantuan microwave memberikan persentase mangiferin lebih tinggi dari metode lain. Tetapi, penggunaan skala besar masih jarang dilakukan karena kemampuan penetrasi microwave yang rendah sehingga ada kemungkinan pemanasan kurang merata.

scholarly journals Identification of 6-methylmercaptopurine derivative formed during acid hydrolysis of thiopurine nucleotides in erythrocytes, using liquid chromatography–mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance assay

1998 ◽  
Vol 44 (12) ◽  
pp. 2511-2515 ◽  
Author(s):  
Thierry Dervieux ◽  
Roselyne Boulieu
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Dihydrogen Phosphate ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Abstract 6-Thioguanine and 6-methylmercaptopurine (Me6-MP) nucleotides are the two major thiopurine metabolites of azathioprine found in erythrocytes. During the acid hydrolysis required for the conversion of thiopurine nucleotides into their free bases, Me6-MP was converted into a compound that could be analyzed on a Purospher RP18-e column with dihydrogen phosphate-methanol buffer as eluent. The pH of the acid extract strongly influenced the conversion of Me6-MP into its derivative. The Me6-MP derivative was identified using liquid chromatography–mass spectrometry and infrared and nuclear magnetic resonance spectrometric methods. During the acid hydrolysis of thiopurine nucleotides in erythrocytes, Me6-MP undergoes degradation, leading to 4-amino-5-(methylthio)carbonyl imidazole.

Sign in / Sign up

Export Citation Format

Share Document