Separation and determination of active components inRadix Salviae miltiorrhizae and its medicinal preparations by nonaqueous capillary electrophoresis

2004 ◽  
Vol 27 (7-8) ◽  
pp. 569-575 ◽  
Author(s):  
An Jia Chen ◽  
Ji You Zhang ◽  
Cun Hong Li ◽  
Xiao Feng Chen ◽  
Zhi De Hu ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Active Components ◽  
Nonaqueous Capillary Electrophoresis ◽  
Salviae Miltiorrhizae ◽  
Medicinal Preparations

Study on Capillary Electrophoresis–Amperometric Detection Profiles from Propolis and its Medicinal Preparations

2013 ◽  
Vol 750-752 ◽  
pp. 1617-1620
Author(s):  
You Yuan Peng
Keyword(s):  
Capillary Electrophoresis ◽  
Phenolic Acids ◽  
Amperometric Detection ◽  
Optimum Conditions ◽  
Active Components ◽  
Resinous Substance ◽  
Medicinal Preparations ◽  
Assay Result ◽  
Pharmacologically Active

Propolis is a resinous substance collected by honeybees from leaf buds and cracks in the bark of various plants, the pharmacologically active components in the propolis are flavonoids and phenolic acids and their esters. A simultaneous determination of flavonoids and phenolic acids from propolis and its medicinal preparations by capillary electrophoresis with electrochemical detection (CE-ED) was reported. Under the optimum conditions, the analytes could be separated in a 100 mmol/l borate buffer (pH=8.7) within 25 min. The response was linear over three orders of magnitude with detection limit (S/N=3) ranging from 1×10-7 g/ml to 5×10-7 g/ml for the analytes. This method has been used for the determination of flavonoids and phenolic acids from propolis and its medicinal preparations, and the assay result was satisfactory.

scholarly journals Determination of active components in Chinese medicinal preparations by capillary electrophoresis

2007 ◽  
Vol 5 (1) ◽  
pp. 221-229 ◽  
Author(s):  
Huitao Liu ◽  
Yuan Gao
Keyword(s):  
Capillary Electrophoresis ◽  
Sodium Dodecylsulfate ◽  
Correlation Coefficients ◽  
Borate Buffer ◽  
Regression Equations ◽  
Active Components ◽  
Medicinal Preparations ◽  
Linear Relationships ◽  
Electrophoretic Behavior

AbstractThe determination of paeoniforin, paeonol, and censenoside Rg1 in traditional Chinese medicinal preparations, Tze Po San Pien pills and Liuwen Dihuang pills has been investigated by micellar electrokinetic capillary electrophoresis with borate buffer (20 mM), sodium dodecylsulfate (30 mM) and acetonitrile (20%) as background electrolytes (pH 9.30), 20 kV applied voltage and 203 nm UV detection. The effects of SDS concentration, borate, buffer pH, and organic modifier on electrophoretic behavior and separation are discussed. Regression equations revealed linear relationships between the peak-area of each component and the content with the correlation coefficients from 0.9982 to 0.9999. In addition, the levels of the active compounds in two kinds of traditional Chinese medicinal preparations were easily determined with the recoveries from 93.1% to 108.2%.

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