Comparison of silylation and esterification/acylation procedures in GC-MS analysis of amino acids

2003 ◽  
Vol 26 (17) ◽  
pp. 1474-1478 ◽  
Author(s):  
Tim G. Sobolevsky ◽  
Alexander I. Revelsky ◽  
Barbara Miller ◽  
Vincent Oriedo ◽  
Elena S. Chernetsova ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ms Analysis

scholarly journals Quantification of amino acids and peptides in an ionic liquid based aqueous two-phase system by LC–MS analysis

AMB Express ◽  
2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Sebastian Oppermann ◽  
Christina Oppermann ◽  
Miriam Böhm ◽  
Toni Kühl ◽  
Diana Imhof ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ionic Liquid ◽  
Phase System ◽  
Two Phase ◽  
Aqueous Two Phase System ◽  
Ms Analysis

Untersuchungen zur Struktur und Derivatisierung von Kondensationsprodukten der 2,4-Diaminobuttersäure mit anderen Aminosäuren / Investigations Regarding Structure and Derivatization of the Condensation Products of 2,4-Diaminobutyric Acid with Other Amino Acids

1994 ◽  
Vol 49 (1-2) ◽  
pp. 18-25 ◽  
Author(s):  
G. Filsak ◽  
K. Taraz ◽  
H. Budzikiewicz
Keyword(s):  
Amino Acids ◽  
Fluorescent Pseudomonads ◽  
Peptide Chain ◽  
Diaminobutyric Acid ◽  
Condensation Products ◽  
Ms Analysis ◽  
Model Substances

Condensation of 2,4-diaminobutyric acid (Dab) with other amino acids yields tetrahydropyrimidine derivatives. Such condensation products can be constituents of the peptide chain of pyoverdins and ferribactins (the siderophors of fluorescent pseudomonads). Synthesis of several representatives and model substances allows to confirm earlier structural conclusions based mainly on NMR evidence. Apparent anomalies accompanying the derivatization for GC/MS analysis could be clarified.

Direct quantitative LC-MS/MS analysis of amino acids in plasma

2019 ◽  
Vol 493 ◽  
pp. S68-S69
Author(s):  
A. Desmons ◽  
E. Thioulouse ◽  
J. Hautem ◽  
A. Saintier ◽  
B. Baudin ◽  
...  
Keyword(s):  
Amino Acids ◽  
Ms Analysis

scholarly journals Quantitative LC–MS/MS analysis of arachidonoyl amino acids in mouse brain with treatment of FAAH inhibitor

2013 ◽  
Vol 432 (2) ◽  
pp. 74-81 ◽  
Author(s):  
Bingnan Han ◽  
Rachel Wright ◽  
Aaron M. Kirchhoff ◽  
Julia A. Chester ◽  
Bruce R. Cooper ◽  
...  
Keyword(s):  
Amino Acids ◽  
Mouse Brain ◽  
Ms Analysis

scholarly journals Profiling of D-alanine production by the microbial isolates of rat gut microbiota

2021 ◽  
Author(s):  
Cindy J. Lee ◽  
Tian A. Qiu ◽  
Zhilai Hong ◽  
Zhenkun Zhang ◽  
Yuhao Min ◽  
...  
Keyword(s):  
Amino Acids ◽  
Gut Microbiota ◽  
Enzymatic Assay ◽  
Male Rats ◽  
Rrna Gene ◽  
Species Variation ◽  
Acid Oxidase ◽  
Amino Acid Oxidase ◽  
Ms Analysis ◽  
Rat Gut

D-alanine (D-Ala) and several other D-amino acids (D-AAs), unusual amino acids present in mammals, act as hormones and neuromodulators in nervous and endocrine systems. Unlike the endogenously synthesized D-serine in animals, D-Ala may be from exogenous sources, e.g., diet and intestinal microorganisms. However, it is unclear if the capability to produce D-Ala and other D-AAs varies among different microbial strains in the gut. We isolated individual microorganisms of rat gut microbiota and profiled their D-AA secretion in vitro, focusing on D-Ala. Serial dilutions of intestinal content from adult male rats were plated on agar to obtain clonal cultures. Using MALDI-TOF MS for rapid strain typing, we identified 38 unique isolates, grouped into 11 species based on 16S rRNA gene sequences. We then used two-tier screening to profile bacterial D-AA secretion, combining a D-amino acid oxidase-based enzymatic assay for rapid assessment of overall D-AA amount, followed by chiral LC-MS/MS to quantify individual D-AAs, revealing 19 out of the 38 isolated strains as D-AA producers. LC-MS/MS analysis of the eight top D-AA producers showed high levels of D-Ala in all strains tested, with substantial inter- and intra-species variations. Though results from enzymatic assay and LC-MS/MS analysis aligned well, LC-MS/MS further revealed the existence of D-glutamate and D-aspartate, which are poor substrates for enzymatic assay. We observed large inter- and intra-species variation of D-AA secretion profiles from rat gut microbiome species, demonstrating the importance of chemical profiling of gut microbiota in addition to sequencing, furthering the idea that microbial metabolites modulate host physiology.

Sign in / Sign up

Export Citation Format

Share Document