Modulation of EGF binding and action by succinylated concanavalin A in fibroblast cell cultures

1980 ◽  
Vol 14 (2) ◽  
pp. 209-214 ◽  
Author(s):  
Kurt Ballmer ◽  
Max M. Burger
Keyword(s):  
Cell Cultures ◽  
Concanavalin A ◽  
Fibroblast Cell

scholarly journals Establishment and characterization of fibroblast cultures derived from a female common hippopotamus (Hippopotamus amphibius) skin biopsy

2020 ◽  
Author(s):  
Tao Wang ◽  
Zelong Li ◽  
Jinpu Wei ◽  
Dongmin Zheng ◽  
Chen Wang ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Cultured Cells ◽  
Population Decline ◽  
Fibroblast Cell ◽  
Population Doubling ◽  
Population Doubling Time ◽  
Fibroblast Cultures ◽  
Hippopotamus Amphibius ◽  
The Common

AbstractThe population decline in the common hippopotamus (Hippopotamus amphibius) has necessitated the preservation of their genetic resources for species conservation and research. Of all actions, cryopreservation of fibroblast cell cultures derived from animal biopsy is considered a simple but efficient means. Nevertheless, preserving viable cell cultures of the common hippopotamus has not been achieved to our knowledge. To this end, we detailed a method to establish fibroblast cell cultures from a female common hippopotamus fetus in this study. By combining the classic tissue explant direct culture and enzymatic digestion methods, we isolated a great number of cells with typical fibroblastic morphology and high viability. Characterization of the fibroblast cultures was carried out using different techniques. In short, neither bacteria/fungi nor mycoplasma was detectable in the cell cultures throughout the study. The population doubling time was 23.9 h according to the growth curve. Karyotyping based on Giemsa staining showed that cultured cells were diploid with 36 chromosomes in all, one pair of which was sex chromosomes. Mitochondrial cytochrome C oxidase subunit I gene sequence of the cultured cells was 99.26% identical with the Hippopotamus amphibius complete mitochondrial DNA sequence registered in GenBank, confirming the cells were derived from a common hippopotamus. Flow cytometry and immunofluorescence staining results revealed that the detected cells were positive for fibroblast markers, S100A4 and Vimentin. In conclusion, we isolated and characterized a new fibroblast cell culture from a common hippopotamus skin sample and the cryopreserved cells could be useful genetic materials for the future research.

scholarly journals Characterisation of a human bilirubin UDP-glucuronosyltransferase stably expressed in hamster lung fibroblast cell cultures

FEBS Letters ◽  
1992 ◽  
Vol 308 (2) ◽  
pp. 161-164 ◽  
Author(s):  
Lesley Sutherland ◽  
Sahidan bin Senafi ◽  
Thomas Ebner ◽  
Douglas J. Clarke ◽  
Brian Burchell
Keyword(s):  
Cell Cultures ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Udp Glucuronosyltransferase

scholarly journals Cyclosporine induces different responses in human epithelial, endothelial and fibroblast cell cultures

2000 ◽  
Vol 58 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Ciro Esposito ◽  
Alessia Fornoni ◽  
Flavia Cornacchia ◽  
Nicoletta Bellotti ◽  
Gianluca Fasoli ◽  
...  
Keyword(s):  
Cell Cultures ◽  
Fibroblast Cell
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