Cytoskeletal elements of chick embryo fibroblasts revealed by detergent extraction

1976 ◽  
Vol 5 (2) ◽  
pp. 119-130 ◽  
Author(s):  
Susan Brown ◽  
Warren Levinson ◽  
James A. Spudich
Keyword(s):  
Chick Embryo ◽  
Detergent Extraction ◽  
Embryo Fibroblasts ◽  
Cytoskeletal Elements

scholarly journals Regulation of integrin mobility and cytoskeletal association in normal and RSV-transformed chick embryo fibroblasts

1990 ◽  
Vol 97 (2) ◽  
pp. 307-315
Author(s):  
A.R. Horvath ◽  
S. Kellie
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Cell Number ◽  
Immunofluorescent Staining ◽  
Integrin Receptor ◽  
Rous Sarcoma ◽  
Surface Configuration ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts ◽  
Cytoskeletal Elements

The mobility of the integrin receptor in trypsinised chick embryo fibroblasts (CEF) was investigated using the CSAT monoclonal antibody. The binding of CSAT to trypsinised CEF followed by incubation at 37 degrees C resulted in patching and then capping of the receptor. This capping was dependent on cellular metabolism, since agents such as sodium azide or 2-deoxyglucose inhibited the process. Whereas about 95% of unclustered integrin was soluble in Nonidet P40-containing buffers, after capping more than 25% of surface integrin became detergent-insoluble, indicating a physical association with cytoskeletal elements. Thus the crosslinking of integrin via its beta subunit is sufficient, to induce cytoskeletal association. Unusually, the microfilament-disrupting drugs cytochalasins B and D potentiated CSAT-induced capping in terms of both cell number and the conformation of caps on individual cells. Double immunofluorescent staining demonstrated that in cytochalasin-treated cells both F-actin and talin co-localised with surface CSAT-integrin clusters. The co-distribution of these cytoskeletal components with surface integrin was retained in cytoskeletal preparations, although there was no quantitative increase of either talin or vinculin in the cytoskeletons. The cocapping of talin with integrin clusters on CEF could also be observed in the absence of cytochalasins. No differences were found in capping efficiency, talin and actin co-localisation or cytoskeletal association of surface-modulated integrin in Rous sarcoma virus (RSV)-transformed cells compared with untransformed counterparts, although differences in the response to cytochalasins were observed. These results provide novel evidence for a physiologically relevant association of integrin with cytoskeletal components and its regulation by surface configuration.(ABSTRACT TRUNCATED AT 250 WORDS)

SEM of non-coated hepatocellular cytoskeleton of perfused livers

1984 ◽  
Vol 42 ◽  
pp. 306-307
Author(s):  
S.W. French ◽  
N.C. Benson ◽  
C. Davis-Scibienski
Keyword(s):  
Ambient Temperature ◽  
Critical Point ◽  
Protease Inhibitors ◽  
Metal Deposition ◽  
Heat Damage ◽  
Detergent Extraction ◽  
Cytoskeletal Elements ◽  
Triton X ◽  
Excised Tissue

Previous SEM studies of liver cytoskeletal elements have encountered technical difficulties such as variable metal coating and heat damage which occurs during metal deposition. The majority of studies involving evaluation of the cell cytoskeleton have been limited to cells which could be isolated, maintained in culture as a monolayer and thus easily extracted. Detergent extraction of excised tissue by immersion has often been unsatisfactory beyond the depth of several cells. These disadvantages have been avoided in the present study. Whole C3H mouse livers were perfused in situ with 0.5% Triton X-100 in a modified Jahn's buffer including protease inhibitors. Perfusion was continued for 1 to 2 hours at ambient temperature. The liver was then perfused with a 2% buffered gluteraldehyde solution. Liver samples including spontaneous tumors were then maintained in buffered gluteraldehyde for 2 hours. Samples were processed for SEM and TEM using the modified thicarbohydrazide procedure of Malich and Wilson, cryofractured, and critical point dried (CPD). Some samples were mechanically fractured after CPD.

scholarly journals Decreased levels of collagen mRNA in rous sarcoma virus-transformed chick embryo fibroblasts

1978 ◽  
Vol 253 (16) ◽  
pp. 5869-5874
Author(s):  
B.H. Howard ◽  
S.L. Adams ◽  
M.E. Sobel ◽  
I. Pastan ◽  
B. de Crombrugghe
Keyword(s):  
Chick Embryo ◽  
Rous Sarcoma Virus ◽  
Collagen Mrna ◽  
Rous Sarcoma ◽  
Sarcoma Virus ◽  
Embryo Fibroblasts

scholarly journals Poly(A) Hydrolase of Chick-Embryo Fibroblasts

1976 ◽  
Vol 65 (2) ◽  
pp. 423-430 ◽  
Author(s):  
Lorraine LEBLOND-LAROUCHE ◽  
Claire DUPUIS ◽  
Rejean MORAIS
Keyword(s):  
Chick Embryo ◽  
Embryo Fibroblasts

Regulation of glucose utilization in chick embryo fibroblasts by bicarbonate ion

1981 ◽  
Vol 107 (2) ◽  
pp. 295-302 ◽  
Author(s):  
Mary H. Miller ◽  
Harold Amos ◽  
Donald A. Sens
Keyword(s):  
Chick Embryo ◽  
Glucose Utilization ◽  
Embryo Fibroblasts
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