Proteins of the hepatoma tissue culture cell plasma membrane

1976 ◽  
Vol 4 (2) ◽  
pp. 141-159 ◽  
Author(s):  
John Tweto ◽  
Else Friedman ◽  
Darrell Doyle
Keyword(s):  
Tissue Culture ◽  
Plasma Membrane ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Cell Plasma Membrane ◽  
Cell Plasma ◽  
Hepatoma Tissue

scholarly journals Tissue-culture Cell fractionation. Zonal centrifugation of Lettrée cells homogenized by glycerol-induced lysis: subfractionation of membranes in sucrose gradients

1979 ◽  
Vol 182 (1) ◽  
pp. 165-171
Author(s):  
J M Graham ◽  
K H M Coffey
Keyword(s):  
Tissue Culture ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Cell Fractionation ◽  
Early Passage ◽  
Late Passage ◽  
Passage Cells ◽  
Zonal Rotor

1. Lettrée cells were grown intraperitoneally in MF-1 mice. 2. Cells that were loaded with glycerol were swollen in 0.1 M-sucrose and disrupted by Dounce homogenization. 3. Early-passage Lettrée cells were more easily disrupted than late-passage cells by this method, and the former produced larger fragments of plasma membrane. 4. The membranes were fractionated initially in sucrose gradients (on the basis of sedimentation rate) in a BXIV zonal rotor. 5. Fractions from this gradient were further resolved in isopycnic sucrose gradients. 6. Plasma-membrane and endoplasmic-reticulum fractions were recovered in good yield and high purity.

A Method for Electron Microscopic Study of Tissue Culture Cell Monolayers Grown in Tubes

1967 ◽  
Vol 25 ◽  
pp. 132-133
Author(s):  
R. Stephens ◽  
G. Schidlovsky ◽  
S. Kuzmic ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Light Microscopy ◽  
Cell Sheet ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Electron Microscopic ◽  
Cell Sheets ◽  
Morphological Alterations ◽  
Culture Cells

The usual method of scraping or trypsinization to detach tissue culture cell sheets from their glass substrate for further pelletization and processing for electron microscopy introduces objectionable morphological alterations. It is also impossible under these conditions to study a particular area or individual cell which have been preselected by light microscopy in the living state.Several schemes which obviate centrifugation and allow the embedding of nondetached tissue culture cells have been proposed. However, they all preserve only a small part of the cell sheet and make use of inverted gelatin capsules which are in this case difficult to handle.We have evolved and used over a period of several years a technique which allows the embedding of a complete cell sheet growing at the inner surface of a tissue culture roller tube. Observation of the same cell by light microscopy in the living and embedded states followed by electron microscopy is performed conveniently.

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