Inhibition of the growth of human melanoma xenografts in nude mice by human tumor-specific cytotoxic T-cells

1990 ◽  
Vol 43 (2) ◽  
pp. 67-72 ◽  
Author(s):  
Nancy J. Crowley ◽  
Craig L. Slingluff ◽  
Carol E. Vervaert ◽  
Timothy L. Darrow ◽  
Hilliard F. Seigler
Keyword(s):  
T Cells ◽  
Nude Mice ◽  
Cytotoxic T Cells ◽  
Human Tumor ◽  
Human Melanoma

scholarly journals IL-6 trans-signaling licenses mouse and human tumor microvascular gateways for trafficking of cytotoxic T cells

2011 ◽  
Vol 121 (10) ◽  
pp. 3846-3859 ◽  
Author(s):  
Daniel T. Fisher ◽  
Qing Chen ◽  
Joseph J. Skitzki ◽  
Jason B. Muhitch ◽  
Lei Zhou ◽  
...  
Keyword(s):  
T Cells ◽  
Cytotoxic T Cells ◽  
Human Tumor

Lentivirus-Mediated Gene Transfer and Expression in Established Human Tumor Antigen-Specific Cytotoxic T Cells and Primary Unstimulated T Cells

2003 ◽  
Vol 14 (11) ◽  
pp. 1089-1105 ◽  
Author(s):  
Xianzheng Zhou ◽  
Yan Cui ◽  
Xin Huang ◽  
Zhiwei Yu ◽  
Amy M. Thomas ◽  
...  
Keyword(s):  
T Cells ◽  
Gene Transfer ◽  
Tumor Antigen ◽  
Cytotoxic T Cells ◽  
Human Tumor

scholarly journals Virus-triggered immune suppression in mice caused by virus-specific cytotoxic T cells.

1988 ◽  
Vol 167 (5) ◽  
pp. 1749-1754 ◽  
Author(s):  
T P Leist ◽  
E Rüedi ◽  
R M Zinkernagel
Keyword(s):  
T Cells ◽  
T Cell ◽  
Nude Mice ◽  
Immune Suppression ◽  
Cytotoxic T Cells ◽  
Lymphocytic Choriomeningitis ◽  
Ifn Alpha ◽  
Infected Cells ◽  
Vesicular Stomatitis

Normal mice infected with 10(5) infectious doses of lymphocytic choriomeningitis virus (LCMV, WE isolate) generated a reduced or no T cell-independent IgM and/or T cell-dependent IgG response to a subsequent vesicular stomatitis virus Indiana (VSV-IND) injection; this transient immune suppression lasted for weeks to months. Connatally infected LCMV-carrier mice or acutely infected T cell-deficient nude mice had normal anti-VSV IgM and IgG or IgM responses respectively. LCMV-infected nude mice transfused with helper cell-depleted LCMV-specific immune spleen cells were immunosuppressed. Normal mice infected with LCMV but treated with a rat anti-CD8 mAb (that had been shown previously to eliminate cytotoxic T cells in vivo) and then infected with VSV exhibited a normal anti-VSV IgM and IgG response. Since no IFN-alpha or -beta was detected on, or after, day 6 of LCMV infection, neither LCMV alone, nor IFN induced by it caused the observed immune suppression; the presented evidence suggests that LCMV-immune CD8+ T cells were responsible for it. It is conceivable that a similar pathogenesis where virus-specific cytotoxic T cells may destroy virus-infected cells essentially involved in an immune response (APC, T helper cells, etc.) may be involved in other virally triggered immune suppression or in AIDS.

scholarly journals Development of Human Neuroblastomas in Mouse-Human Neural Crest Chimeras

2019 ◽  
Author(s):  
Malkiel A. Cohen ◽  
Shupei Zhang ◽  
Satyaki Sengupta ◽  
Haiting Ma ◽  
Brendan Horton ◽  
...  
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Neural Crest ◽  
Human Cancer ◽  
Cytotoxic T Cells ◽  
Human Tumor ◽  
Model System ◽  
Mouse Embryos ◽  
Immune Checkpoints ◽  
Experimental Platform

SummaryNeuroblastoma (NB), derived from the neural crest (NC), is the most common pediatric extracranial solid tumor. Here we establish a platform that allows studying human NBs in mouse-human NC chimeras. Chimeric mice were produced by injecting human NC cells carrying NB relevant oncogenes in-utero into gastrulating mouse embryos. The mice developed tumors composed of a heterogenous cell population that closely resembled that seen in primary NBs of patients but were significantly different from homogenous tumors formed in xenotransplantation models. The human tumors emerged in immunocompetent hosts and were extensively infiltrated by mouse cytotoxic T cells reflecting a vigorous host anti-tumor immune response. However, the tumors blunted the immune response by inducing infiltration of regulatory T cells and expression of immune checkpoints similar to escape mechanisms seen in human cancer patients. Thus, this experimental platform allows studying human tumor initiation, progression, manifestation and tumor – immune-system interactions in an animal model system.

scholarly journals Engineering Human Tumor-specific Cytotoxic T Cells to Function in a Hypoxic Environment

2008 ◽  
Vol 16 (3) ◽  
pp. 599-606 ◽  
Author(s):  
Hongsung Kim ◽  
Guangyong Peng ◽  
John M Hicks ◽  
Heidi L Weiss ◽  
Erwin G Van Meir ◽  
...  
Keyword(s):  
T Cells ◽  
Cytotoxic T Cells ◽  
Human Tumor ◽  
Hypoxic Environment

scholarly journals Restriction specificities, alloreactivity, and allotolerance expressed by T cells from nude mice reconstituted with H-2-compatible or -incompatible thymus grafts.

1980 ◽  
Vol 151 (2) ◽  
pp. 376-399 ◽  
Author(s):  
R M Zinkernagel ◽  
A Althage ◽  
E Waterfield ◽  
B Kindred ◽  
R M Welsh ◽  
...  
Keyword(s):  
T Cells ◽  
Nude Mice ◽  
Competitive Inhibition ◽  
Cytotoxic T Cells ◽  
Fetal Thymus ◽  
Adult Mice ◽  
Donor Type ◽  
Adult Thymus

Congenitally thymusless nude mice that lacked functional T cells were reconstituted with H-2-compatible or -incompatible thymus grafts taken from either fetal, newborn, or adult mice and transplanted under the kidney capsule or subcutaneously. Transplantation with unirradiated fetal (15--17 d) or newborn thymus grafts reconstituted the nude mice as assessed by their subsequent generation of virus-specific cytotoxic T cells in vivo or alloreactive T cells in vitro. The restriction specificity of T cells from homozygous mice was exclusively for the nude host H-2, as shown by direct cytolysis or by cold target competitive inhibition assays. irrespective of whether nude mice were reconstituted with H-2-compatible, semiallogeneic, or H-2-incompatible, unirradiated newborn or fetal thymus grafts (in order of decreasing efficiency of reconstitution). The restriction specificity for the nonhost H-2 of the thymus could not be demonstrated even after primary or secondary sensitization in an infected appropriate F1 environment. These nude mice reconstituted with fetal or newborn grafts were tolerant to the H-2 of the thymus donors. Nude mice transplanted with irradiated adult thymus grafts were reconstituted functionally with syngeneic or semisyngeneic but not with allogeneic thymus grafts. In homozygous nu/nu irradiated heterozygous recipients of F1 thymus grafts, the restriction specificity for the nonhost thymic H-2 could not be elicited upon adoptive sensitization in irradiated and infected F1 heterozygote stimulator mice; in fact, these chimeras' lymphocytes were not tolerant to the nonhost H-2. The discrepancy between the restorative capacity of unirradiated vs. irradiated thymus grafts suggests that precursors of T cells in nude mice can acquire restriction specificity and immunocompetence independently of a conventional, functioning H-2-compatible thymus if exposed to an allogeneic fetal or a newborn thymus that contains functioning thymocytes of donor type but not if reconstituted with an irradiated adult allogeneic thymus.

scholarly journals Human Telomerase Reverse Transcriptase-Transduced Human Cytotoxic T Cells Suppress the Growth of Human Melanoma in Immunodeficient Mice

2004 ◽  
Vol 64 (6) ◽  
pp. 2153-2161 ◽  
Author(s):  
Natascha C. V. Verra ◽  
Annelies Jorritsma ◽  
Kees Weijer ◽  
Janneke J. Ruizendaal ◽  
Arie Voordouw ◽  
...  
Keyword(s):  
T Cells ◽  
Reverse Transcriptase ◽  
Cytotoxic T Cells ◽  
Human Melanoma ◽  
Telomerase Reverse Transcriptase ◽  
Human Telomerase Reverse Transcriptase ◽  
Immunodeficient Mice ◽  
Human Telomerase
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