A more sensitive detection of micrometastases of NSCLC in lymph nodes using the one-step nucleic acid amplification (OSNA) method

2017 ◽  
Vol 117 (2) ◽  
pp. 163-170 ◽  
Author(s):  
Josef Vodicka ◽  
Petr Mukensnabl ◽  
Sarka Vejvodova ◽  
Vladimir Spidlen ◽  
Vlastimil Kulda ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lymph Nodes ◽  
Nucleic Acid Amplification ◽  
Sensitive Detection ◽  
One Step ◽  
The One ◽  
Osna Method

scholarly journals Detection of sentinel lymph node metastases in cervical cancer: Assessment of KRT19 mRNA in the one-step nucleic acid amplification (OSNA) method

2013 ◽  
Vol 130 (3) ◽  
pp. 530-536 ◽  
Author(s):  
Satoshi Okamoto ◽  
Hitoshi Niikura ◽  
Kadzuki Nakabayashi ◽  
Kayo Hiyama ◽  
Maki Matoda ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Lymph Node ◽  
Nucleic Acid ◽  
Sentinel Lymph Node ◽  
Lymph Node Metastases ◽  
Nucleic Acid Amplification ◽  
One Step ◽  
Node Metastases ◽  
The One ◽  
Osna Method

Assessment of suitability of the one step nucleic acid amplification (OSNA) assay as an intraoperative procedure for detection of metastasis in sentinel lymph nodes of breast cancer

2014 ◽  
Vol 67 (12) ◽  
pp. 1032-1037 ◽  
Author(s):  
Ana Richelia Jara-Lazaro ◽  
Ilyana Huda Mohamed Hussain ◽  
Aye Aye Thike ◽  
Chow Yin Wong ◽  
Gay Hui Ho ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nucleic Acid ◽  
Lymph Nodes ◽  
False Negative ◽  
Turnaround Time ◽  
Sentinel Lymph Nodes ◽  
Nucleic Acid Amplification ◽  
One Step ◽  
The One ◽  
Osna Assay

AimWe aimed to assess the one step nucleic acid amplification (OSNA) assay as an intraoperative method in comparison with frozen sections (FS) for detection of metastasis in sentinel lymph nodes (SLNs) of breast cancer.Method100 SLNs from patients with breast carcinoma were enrolled within a 3-month period. Alternate 2 mm node slices were subjected to routine FS, and later to permanent histology, and the rest for automated molecular detection of CK19 mRNA using OSNA. FS and OSNA findings were compared with permanent histology results. Difference in turnaround time was also noted.ResultsWith permanent histology as gold standard, OSNA was discrepant in 8 of 98 (3 false negative, 5 false positive) included SLNs whereas FS had 2 false negative cases. FS had higher sensitivity (89%, p=<0.001), specificity (100%, p=0.001) and concordance rate (98%) than OSNA (83%, 94% and 92%, respectively). FS showed almost perfect agreement (κ=0.929) whereas OSNA showed substantial agreement (κ=0.740) when compared with permanent histology. OSNA turnaround time was twice longer (mean of 47.7 min) than FS.ConclusionsAutomation of SLN assessment using OSNA is a potentially useful intraoperative diagnostic tool with acceptable accuracy. Discordant findings in this study may be due to sampling allocation. Since OSNA is more time-consuming, its practical advantage over routine FS requires further study in view of current technical workflow considerations.

Implementation of One Step Nucleic acid Amplification (OSNA) for Intra-operative assessment of sentinel lymph nodes in a DGH

2012 ◽  
Vol 38 (5) ◽  
pp. 439-440 ◽  
Author(s):  
Mohd Iqbal ◽  
Ali Jibran Mecci ◽  
Lisa Whisker ◽  
Michael Parkes ◽  
Adrian Smith ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Lymph Nodes ◽  
Sentinel Lymph Nodes ◽  
Nucleic Acid Amplification ◽  
One Step ◽  
Operative Assessment

scholarly journals Elucidation of inhibitory effects on metastatic sentinel lymph nodes of breast cancer during One-Step Nucleic Acid Amplification

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Yoshiya Horimoto ◽  
Masahiko Tanabe ◽  
Saiko Kazuno ◽  
Yoshiki Miura ◽  
Kaoru Mogushi ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Nucleic Acid ◽  
Lymph Nodes ◽  
Sentinel Lymph Nodes ◽  
Nucleic Acid Amplification ◽  
Inhibitory Effects ◽  
One Step

The Diagnostic Value of One-Step Nucleic acid Amplification (OSNA) for Sentinel Lymph Nodes in Colon Cancer Patients

2014 ◽  
Vol 21 (12) ◽  
pp. 3924-3930 ◽  
Author(s):  
F. J. Vogelaar ◽  
M. S. Reimers ◽  
R. L. A. van der Linden ◽  
J. C. van der Linden ◽  
V. T. H. B. M. Smit ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Nucleic Acid ◽  
Lymph Nodes ◽  
Cancer Patients ◽  
Diagnostic Value ◽  
Sentinel Lymph Nodes ◽  
Nucleic Acid Amplification ◽  
One Step
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