Novel focal adhesion protein kindlin-2 promotes the invasion of gastric cancer cells through phosphorylation of integrin β1 and β3

2013 ◽  
Vol 108 (2) ◽  
pp. 106-112 ◽  
Author(s):  
Zhanlong Shen ◽  
Yingjiang Ye ◽  
Tuuli Kauttu ◽  
Hanna Seppänen ◽  
Sanna Vainionpää ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Focal Adhesion ◽  
Integrin Β1 ◽  
Gastric Cancer Cells ◽  
Adhesion Protein ◽  
Focal Adhesion Protein

scholarly journals M2 Macrophages Mediate the Resistance of Gastric Adenocarcinoma Cells to 5-Fluorouracil through the Expression of Integrin β3, Focal Adhesion Kinase, and Cofilin

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Daniel Ngabire ◽  
Irvine Niyonizigiye ◽  
Maheshkumar Prakash Patil ◽  
Yeong-Ae Seong ◽  
Yong Bae Seo ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Focal Adhesion ◽  
Gastric Cancer Cells ◽  
Ags Cells ◽  
Alternatively Activated Macrophages ◽  
Adhesion Protein ◽  
Integrin Β3 ◽  
Cancer Invasiveness ◽  
Gastric Cancer Treatment ◽  
Focal Adhesion Protein

Tumor microenvironment components dictate the growth and progression of various cancers. Tumor-associated macrophages are the most predominant cells in TME and play a major role in cancer invasiveness. Gastric cancer is one of the most common cancers in Asia, and recently, various cases of resistance to fluorouracil treatment have been reported. In this study, we investigated the role of alternatively activated macrophages in the resistance of AGS gastric cancer cells to fluorouracil. THP-1 cells were polarized using recombinant human IL-4, then were cocultured with AGS cells treated with fluorouracil. Cell viability, Western blot, immunofluorescence, and cell invasion were performed for this investigation. Our results demonstrated that polarized macrophages initiated the survival of treated AGS cells and induced the resistance in AGS by upregulating the expression of integrin β3, focal adhesion protein (FAK), and cofilin proteins. These results reveal that integrin β3, focal adhesion protein (FAK), and cofilin proteins are potential targets for the improvement of fluorouracil efficacy in gastric cancer treatment.

scholarly journals The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages

2012 ◽  
Vol 29 (2) ◽  
pp. 791-797 ◽  
Author(s):  
ZHANLONG SHEN ◽  
YINGJIANG YE ◽  
TUULI KAUTTU ◽  
HANNA SEPPÄNEN ◽  
SANNA VAINIONPÄÄ ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Focal Adhesion ◽  
The Novel ◽  
Tumor Associated Macrophages ◽  
Gastric Cancer Cells

scholarly journals Dual phenotype of MDA-MB-468 cancer cells reveals mutual regulation of tensin3 and adhesion plasticity

2017 ◽  
Author(s):  
Astrid Veβ ◽  
Ulrich Blache ◽  
Laura Leitner ◽  
Angela R.M. Kurz ◽  
Anja Ehrenpfordt ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Focal Adhesion ◽  
Three Dimensional ◽  
Suspension Cells ◽  
Adhesion Protein ◽  
Cell Matrix ◽  
Mutual Regulation ◽  
Summary Statement ◽  
And Migration ◽  
Focal Adhesion Protein

AbstractPlasticity between adhesive and less-adhesive states is important for mammalian cell behaviour. To investigate adhesion plasticity, we have selected a stable isogenic subpopulation of MDA-MB-468 breast carcinoma cells which grows in suspension. These suspension cells are unable to re-adhere to various matrices or to contract three-dimensional collagen lattices. By transcriptome analysis, we identified the focal adhesion protein tensin3 (Tns3) as a determinant of adhesion plasticity. Tns3 is strongly reduced on mRNA and protein level in suspension cells. Furthermore, challenging breast cancer cells transiently with non-adherent conditions markedly reduces Tns3 expression, which is regained upon re-adhesion. Stable knockdown of Tns3 in parental cells results in defective adhesion, spreading and migration. Tns3 knockdown cells display impaired structure and dynamics of focal adhesion complexes as determined by immunostaining. Restoration of Tns3 expression in suspension cells partially rescues adhesion and focal contact composition. Our work identifies Tns3 as a critical focal adhesion component regulated by, and functionally contributing to, the switch between adhesive and non-adhesive states in MDA-MB-468 cancer cells.Summary statementWe identify the cell-matrix adapter protein tensin3 as a determinant of adhesion plasticity, using cancer cells selected for non-adherent growth. Tensin3 expression constitutes a feedback loop controlling adhesion and motility.

Alpha-fetoprotein producing gastric cancer cells lack transcription factor AT-motif binding factor 1 (ATBF1)

2001 ◽  
Vol 120 (5) ◽  
pp. A31-A31
Author(s):  
H KATAOKA ◽  
T JOH ◽  
T OHSHIMA ◽  
Y ITOH ◽  
K SENOO ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Transcription Factor ◽  
Cancer Cells ◽  
Alpha Fetoprotein ◽  
Gastric Cancer Cells ◽  
Binding Factor

Distinct mechanism of helicobacter pylori-mediated NF-κB activation between gastric cancer cells, MKN45 and monocystic cells, THP-1

2001 ◽  
Vol 120 (5) ◽  
pp. A82-A82 ◽  
Author(s):  
S MAEDA ◽  
Y MITSUNO ◽  
Y HIRATA ◽  
M AKANUMA ◽  
H YOSHIDA ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Helicobacter Pylori ◽  
Cancer Cells ◽  
Gastric Cancer Cells ◽  
Distinct Mechanism

Hepatocyte Growth Factor Promotes Cell Survival by Phosphorylation of BAD in Gastric Cancer Cells

2008 ◽  
Vol 17 (1) ◽  
pp. 23-32 ◽  
Author(s):  
Kyung Hee Lee ◽  
Eun Young Choi ◽  
Min Kyoung Kim ◽  
Myung Soo Hyun ◽  
Jong Ryul Eun ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Survival ◽  
Cancer Cells ◽  
Gastric Cancer Cells ◽  
Hepatocyte Growth

Silencing of circRACGAP1 Sensitizes Gastric Cancer Cells to Apatinib via Modulating Autophagy by Targeting miR-3657 and ATG7

2019 ◽  
Author(s):  
Ling Ma ◽  
Zhangding Wang ◽  
Mengyan Xie ◽  
Weiyou Zhu ◽  
Fengming Yang ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cancer Cells ◽  
Gastric Cancer Cells
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