Microflora of fresh white button mushrooms ( Agaricus bisporus ) during cold storage revealed by high‐throughput sequencing and MALDI‐TOF mass spectrometry fingerprinting

2019 ◽  
Vol 99 (9) ◽  
pp. 4498-4503 ◽  
Author(s):  
Wanwei Qiu ◽  
Ying Huang ◽  
Chao Zhao ◽  
Zhenshan Lin ◽  
Wenxing Lin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Cold Storage ◽  
Agaricus Bisporus ◽  
High Throughput Sequencing ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Button Mushrooms ◽  
White Button Mushrooms

scholarly journals High-throughput killer cell immunoglobulin-like receptor genotyping by MALDI-TOF mass spectrometry with discovery of novel alleles

2007 ◽  
Vol 59 (7) ◽  
pp. 525-537 ◽  
Author(s):  
Kathleen A. Houtchens ◽  
Robert J. Nichols ◽  
Martha B. Ladner ◽  
Hannah E. Boal ◽  
Cristina Sollars ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Killer Cell ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Novel Alleles

scholarly journals genotools SNP MANAGER: A New Software for Automated High-Throughput MALDI-TOF Mass Spectrometry SNP Genotyping

BioTechniques ◽  
2001 ◽  
Vol 30 (1) ◽  
pp. 210-215 ◽  
Author(s):  
W. Pusch ◽  
K.-O. Kraeuter ◽  
T. Froehlich ◽  
Y. Stalgies ◽  
M. Kostrzewa
Keyword(s):  
Mass Spectrometry ◽  
High Throughput ◽  
Snp Genotyping ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof

scholarly journals High-Throughput Quantitative Profiling of Serum N-Glycome by MALDI-TOF Mass Spectrometry and N-Glycomic Fingerprint of Liver Fibrosis

2007 ◽  
Vol 53 (7) ◽  
pp. 1254-1263 ◽  
Author(s):  
Richard KT Kam ◽  
Terence CW Poon ◽  
Henry LY Chan ◽  
Nathalie Wong ◽  
Alex Y Hui ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liver Fibrosis ◽  
High Throughput ◽  
Quantitative Aspect ◽  
Diagnostic Model ◽  
Maldi Tof Ms ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Ishak Score ◽  
Tof Ms

Abstract Background: The use of MALDI-TOF mass spectrometry (MS) in quantitative glycan profiling has not been reported. In this study, we attempted to establish a high-throughput quantitative assay for profiling serum N-glycome, and we applied the new assay to identifying serum N-glycans for diagnosis of liver fibrosis and cirrhosis. Methods: N-glycans from whole serum proteins in 2 μL serum were released by enzymatic digestion, cleaned up by hydrophilic chromatography, and subsequently quantitatively profiled with a linear MALDI-TOF MS system, which was originally designed for quantitative proteomic profiling. Serum N-glycome profiles from 46 patients with chronic hepatitis B infection and with different degrees of liver fibrosis were examined. Results: The intra- and interassay CVs of peak intensities of the standard N-glycans were <8% and <17%, respectively. When the assay was applied to the analysis of serum N-glycome profiles, 17 peaks were found to be potential biomarkers for detection of liver fibrosis/cirrhosis. Linear regression analysis revealed that 4 peaks of 1341.5, 1829.7, 1933.3, and 2130.3 m/z (all P <0.005) had complementary value in detecting liver fibrosis and included them, but not any serological markers, in the diagnostic model. Leave-one-out cross-validation showed the diagnostic model could identify significant fibrosis (Ishak score ≥3) and cirrhosis (Ishak score ≥5), both at 85% accuracy. Conclusion: This is the first study to illustrate the quantitative aspect of MALDI-TOF MS in N-glycome profiling and the first study to reveal the potential value of the serum N-glycan profile for identifying liver fibrosis.

scholarly journals Combining high-throughput MALDI-TOF mass spectrometry and isoelectric focusing gel electrophoresis for virtual 2D gel-based proteomics

Methods ◽  
2016 ◽  
Vol 104 ◽  
pp. 163-169 ◽  
Author(s):  
Karen Lohnes ◽  
Neil R. Quebbemann ◽  
Kate Liu ◽  
Fred Kobzeff ◽  
Joseph A. Loo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Isoelectric Focusing ◽  
High Throughput ◽  
Gel Electrophoresis ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
2D Gel

MALDI-TOF Mass Spectrometry-Based High-Throughput Screening for Inhibitors of the Cytosolic DNA Sensor cGAS

2019 ◽  
Vol 25 (4) ◽  
pp. 372-383 ◽  
Author(s):  
Roman P. Simon ◽  
Martin Winter ◽  
Carola Kleiner ◽  
Robert Ries ◽  
Gisela Schnapp ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
High Throughput ◽  
Cyclic Gmp ◽  
High Throughput Screening ◽  
Detection Methods ◽  
Maldi Tof Mass Spectrometry ◽  
Maldi Tof ◽  
Discovery Research ◽  
Drug Discovery Research

Comprehensive and unbiased detection methods are a prerequisite for high-throughput screening (HTS) campaigns within drug discovery research. Label-free matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has been introduced as an HTS-compatible readout for biochemical test systems to support the drug discovery process. So far, reported HTS applications were based on surface-modified systems or proof-of-concept studies. We present the utilization of a MALDI-TOF-based screening platform to identify inhibitors of human cyclic GMP-AMP synthase (cGAS), a mediator of innate immune response whose aberration has been causally correlated to a number of inflammatory disorders. In this context, the development and validation of a MALDI-TOF-based activity assay is reported to demonstrate fast, robust, and accurate detection of chemical cGAS inhibition by direct quantification of the physiological reaction product cyclic GMP-ATP (cGAMP). Results from a screen of a diverse library of more than 1 million small molecules in 1536-well format against the catalytic cGAS activity are presented with excellent assay performance and data quality. Identified hits were qualified in dose–response experiments and confirmed by RapidFire-MS measurements. Conclusively, the presented data provide the first proof of applicability of direct automated MALDI-TOF MS as a readout strategy for large-scale drug discovery HTS campaigns.

Sign in / Sign up

Export Citation Format

Share Document