Separation, biochemical characterization and salt‐tolerant mechanisms of alkaline protease from Aspergillus oryzae

2019 ◽  
Vol 99 (7) ◽  
pp. 3359-3366 ◽  
Author(s):  
Xianli Gao ◽  
Yiyun Yin ◽  
Jingkun Yan ◽  
Junke Zhang ◽  
Haile Ma ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Alkaline Protease ◽  
Biochemical Characterization ◽  
Salt Tolerant

Enhancing Activities of Salt-Tolerant Proteases Secreted by Aspergillus oryzae Using Atmospheric and Room-Temperature Plasma Mutagenesis

2020 ◽  
Vol 68 (9) ◽  
pp. 2757-2764 ◽  
Author(s):  
Xianli Gao ◽  
Ermeng Liu ◽  
Yiyun Yin ◽  
Lixin Yang ◽  
Qingrong Huang ◽  
...  
Keyword(s):  
Aspergillus Oryzae ◽  
Room Temperature ◽  
Salt Tolerant ◽  
Temperature Plasma

Salt-tolerant and thermostable alkaline protease fromBacillus subtilis NCIM No. 64

1993 ◽  
Vol 38 (1-2) ◽  
pp. 83-92 ◽  
Author(s):  
Asha A. Kembhavi ◽  
Anuradha Kulkarni ◽  
Aditi Pant
Keyword(s):  
Alkaline Protease ◽  
Salt Tolerant

scholarly journals Protease from Aspergillus oryzae: Biochemical Characterization and Application as a Potential Biocatalyst for Production of Protein Hydrolysates with Antioxidant Activities

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Ruann Janser Soares de Castro ◽  
Helia Harumi Sato
Keyword(s):  
Aspergillus Oryzae ◽  
Biochemical Characterization ◽  
Antioxidant Activities ◽  
Soy Protein Isolate ◽  
Antioxidant Properties ◽  
Protein Isolate ◽  
Protein Hydrolysates ◽  
Optimum Temperature ◽  
Temperature Range ◽  
Ph Range

This study reports the biochemical characterization of a protease from Aspergillus oryzae LBA 01 and the study of the antioxidant properties of protein hydrolysates produced with this protease. The biochemical characterization showed that the enzyme was most active over the pH range 5.0–5.5 and was stable from pH 4.5 to 5.5. The optimum temperature range for activity was 55–60°C, and the enzyme was stable at temperatures below 45°C. The activation energy (Ea) for azocasein hydrolysis and temperature quotient (Q10) were found to be 37.98 kJ mol−1 and 1.64–1.53 at temperature range from 30 to 55°C, respectively. The enzyme exhibited t1/2 of 97.63 min and a D value of 324.31 at the optimum temperature for activity (57.2°C). Protease from A. oryzae LBA 01 was shown as a potentially useful biocatalyst for protein hydrolysis, increasing the antioxidant activities of soy protein isolate, bovine whey protein, and egg white protein from 2.0- to 10.0-fold.

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